Myristic Acid (MA) Promotes Adipogenic Gene Expression and the Differentiation of Porcine Intramuscular Adipocyte Precursor Cells

doi:10.1016/S2095-3119(13)60664-4

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (11): 2488-2499.DOI: 10.1016/S2095-3119(13)60664-4

Myristic Acid (MA) Promotes Adipogenic Gene Expression and the Differentiation of Porcine Intramuscular Adipocyte Precursor Cells

LU Nai-sheng, SHU Gang, XIE Qiu-ping, ZHU Xiao-tong, GAO Ping, ZHOU Gui-xuan, WANG Songbo, WANG Li-na, XI Qian-yun, ZHANG Yong-liang , JIANG Qing-yan

College of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R.China

收稿日期:2013-06-25 出版日期:2014-11-06 发布日期:2014-11-14
通讯作者: JIANG Qing-yan, Tel: +86-20-85284930, Fax: +86-20-85284901, E-mail: qyjiang@scau.edu.cn
作者简介:LU Nai-sheng, E-mail: 236884381@qq.com
基金资助:
the financial support of this work, which was provided by the National Basic Research Program of China (2012CB124701), the National Natural Science Foundation of China (30972157), the Natural Science Foundation of Guangdong Province, China (S2012020011048) and the Talent Project of Guangdong Colleges, China.

Myristic Acid (MA) Promotes Adipogenic Gene Expression and the Differentiation of Porcine Intramuscular Adipocyte Precursor Cells

LU Nai-sheng, SHU Gang, XIE Qiu-ping, ZHU Xiao-tong, GAO Ping, ZHOU Gui-xuan, WANG Songbo, WANG Li-na, XI Qian-yun, ZHANG Yong-liang , JIANG Qing-yan

College of Animal Sciences, South China Agricultural University, Guangzhou 510642, P.R.China

Received:2013-06-25 Online:2014-11-06 Published:2014-11-14
Contact: JIANG Qing-yan, Tel: +86-20-85284930, Fax: +86-20-85284901, E-mail: qyjiang@scau.edu.cn
About author:LU Nai-sheng, E-mail: 236884381@qq.com
Supported by:
the financial support of this work, which was provided by the National Basic Research Program of China (2012CB124701), the National Natural Science Foundation of China (30972157), the Natural Science Foundation of Guangdong Province, China (S2012020011048) and the Talent Project of Guangdong Colleges, China.

摘要/Abstract

摘要： Intramuscular fat (IMF) content is considered to be a key factor that affects the marbling, tenderness, juiciness and flavor of pork. To investigate the effects of myristic acid (MA) on the differentiation of porcine intramuscular adipocytes, cells were isolated from longissimus dorsi muscle (LDM) and treated with 0, 10, 50 or 100 μmol L-1 MA. The results showed that MA significantly promotes the differentiation of intramuscular adipocytes in a dose-dependent manner. MA also led to a parallel increase in the expression of peroxisome proliferator activated receptor-γ (PPARγ) and adipose-related genes, such as glucose transporter 1 (GLUT1), lipoprotein lipase (LPL), adipocyte fatty acid binding protein 4 (FABP4/aP2), fatty acid translocase (FAT), acetyl-CoA carboxylase α (ACCα), adipose triglyceride lipase (ATGL) and fatty acid synthase (FASN). However, no significant effects of MA were observed on the expression of CAAT enhancer binding protein-α (C/EBPα) or hormone sensitive lipase (HSL). The expression of pyruvate dehydrogenase kinase 4 (PDK4) was increased by MA during the early stages of differentiation (day 1-3). In addition, MA also increased the absolute content of C14 (P<0.001) and saturated fatty acids (SFA) (P<0.05) to varying degrees, but no effects were observed on other fatty acids. These results suggest that MA might be able to enhance the IMF content of pork and increase the accumulation of myristic and myristoleic acid in muscle, which might have beneficial implications for human health.

关键词: porcine , intramuscular fat , myristic acid , adipogenesis , fatty acid composition

Abstract: Intramuscular fat (IMF) content is considered to be a key factor that affects the marbling, tenderness, juiciness and flavor of pork. To investigate the effects of myristic acid (MA) on the differentiation of porcine intramuscular adipocytes, cells were isolated from longissimus dorsi muscle (LDM) and treated with 0, 10, 50 or 100 μmol L-1 MA. The results showed that MA significantly promotes the differentiation of intramuscular adipocytes in a dose-dependent manner. MA also led to a parallel increase in the expression of peroxisome proliferator activated receptor-γ (PPARγ) and adipose-related genes, such as glucose transporter 1 (GLUT1), lipoprotein lipase (LPL), adipocyte fatty acid binding protein 4 (FABP4/aP2), fatty acid translocase (FAT), acetyl-CoA carboxylase α (ACCα), adipose triglyceride lipase (ATGL) and fatty acid synthase (FASN). However, no significant effects of MA were observed on the expression of CAAT enhancer binding protein-α (C/EBPα) or hormone sensitive lipase (HSL). The expression of pyruvate dehydrogenase kinase 4 (PDK4) was increased by MA during the early stages of differentiation (day 1-3). In addition, MA also increased the absolute content of C14 (P<0.001) and saturated fatty acids (SFA) (P<0.05) to varying degrees, but no effects were observed on other fatty acids. These results suggest that MA might be able to enhance the IMF content of pork and increase the accumulation of myristic and myristoleic acid in muscle, which might have beneficial implications for human health.

Key words: porcine , intramuscular fat , myristic acid , adipogenesis , fatty acid composition

LU Nai-sheng, SHU Gang, XIE Qiu-ping, ZHU Xiao-tong, GAO Ping, ZHOU Gui-xuan, WANG Songbo, WANG Li-na, XI Qian-yun, ZHANG Yong-liang , JIANG Qing-yan. Myristic Acid (MA) Promotes Adipogenic Gene Expression and the Differentiation of Porcine Intramuscular Adipocyte Precursor Cells[J]. Journal of Integrative Agriculture, 2014, 13(11): 2488-2499.

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Myristic Acid (MA) Promotes Adipogenic Gene Expression and the Differentiation of Porcine Intramuscular Adipocyte Precursor Cells

Myristic Acid (MA) Promotes Adipogenic Gene Expression and the Differentiation of Porcine Intramuscular Adipocyte Precursor Cells

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