Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.)

doi:10.1016/S2095-3119(13)60575-4

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (8): 1609-1620.DOI: 10.1016/S2095-3119(13)60575-4

• 论文 • 下一篇

Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.)

CHAILu1, ZHANGJian1, PANXiao-biao2, ZHANGFan1, ZHENGTian-qing1, ZHAOXiu-qing1, WANGWen-sheng1, AliJauhar3, XUJian-long1, LIZhi-kang1

1、National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2、Crop Research Institute, Taizhou Academy of Agricultural Sciences of Zhejiang Province, Linhai 317000, P.R.China
3、International Rice Research Institute, DAPO 777, Metro Malina, The Philippines

收稿日期:2013-05-17 出版日期:2014-08-01 发布日期:2014-08-02
通讯作者: LI Zhi-kang, Tel: +86-10-82105857, E-mail: zhkli1953@126.com; XU Jian-long, Tel: +86-10-82105854, Fax: +86-10-82108559, E-mail: xujlcaas@126.com
基金资助:
Acknowledgements This research study was funded by the National High-Tech R&D Program of China (2012AA101101), the 948 Project from the Ministry of Agriculture, China (2010-G2B), the International Cooperative Project from the Ministry of Science and Technology, China (S2012ZR0160), and the Bill & Melinda Gates Foundation Project (OPP51587).

Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.)

CHAILu1 , ZHANGJian1 , PANXiao-biao2 , ZHANGFan1 , ZHENGTian-qing1 , ZHAOXiu-qing1 , WANGWen-sheng1 , AliJauhar3 , XUJian-long1 , LIZhi-kang1

1、National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2、Crop Research Institute, Taizhou Academy of Agricultural Sciences of Zhejiang Province, Linhai 317000, P.R.China
3、International Rice Research Institute, DAPO 777, Metro Malina, The Philippines

Received:2013-05-17 Online:2014-08-01 Published:2014-08-02
Contact: LI Zhi-kang, Tel: +86-10-82105857, E-mail: zhkli1953@126.com; XU Jian-long, Tel: +86-10-82105854, Fax: +86-10-82108559, E-mail: xujlcaas@126.com
Supported by:
Acknowledgements This research study was funded by the National High-Tech R&D Program of China (2012AA101101), the 948 Project from the Ministry of Agriculture, China (2010-G2B), the International Cooperative Project from the Ministry of Science and Technology, China (S2012ZR0160), and the Bill & Melinda Gates Foundation Project (OPP51587).

摘要/Abstract

摘要： Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the whole- plant growth duration salt tolerance (ST) was genetically dissected by phenotyping two sets of BC2F5 introgression lines (ILs) for four yield traits under severe natural salt stress and non-stress filed conditions using SSR markers and the methods of advanced backcross QTL (AB-QTL) analysis and selective introgression. Many QTLs affecting four yield traits under salt stress and non- stress conditions were identified, most (>90%) of which were clustered in 13 genomic regions of the rice genome and involved in complex epistasis. Most QTLs affecting yield traits were differentially expressed under salt stress and non-stress conditions. Our results suggested that genetics complementarily provides an adequate explanation for the hidden genetic diversity for ST observed in both IL populations. Some promising Huanghuazhan (HHZ) ILs with favorable donor alleles at multiple QTLs and significantly improved yield traits under salt stress and non-stress conditions were identified, providing excellent materials and relevant genetic information for improving rice ST by marker-assisted selection (MAS) or genome selection.

关键词: rice , whole plant growth duration salt tolerance , quantitative trait loci (QTLs) , epistasis

Abstract: Salinity is a major factor limiting rice yield in coastal areas of Asia. To facilitate breeding salt tolerant rice varieties, the whole- plant growth duration salt tolerance (ST) was genetically dissected by phenotyping two sets of BC2F5 introgression lines (ILs) for four yield traits under severe natural salt stress and non-stress filed conditions using SSR markers and the methods of advanced backcross QTL (AB-QTL) analysis and selective introgression. Many QTLs affecting four yield traits under salt stress and non- stress conditions were identified, most (>90%) of which were clustered in 13 genomic regions of the rice genome and involved in complex epistasis. Most QTLs affecting yield traits were differentially expressed under salt stress and non-stress conditions. Our results suggested that genetics complementarily provides an adequate explanation for the hidden genetic diversity for ST observed in both IL populations. Some promising Huanghuazhan (HHZ) ILs with favorable donor alleles at multiple QTLs and significantly improved yield traits under salt stress and non-stress conditions were identified, providing excellent materials and relevant genetic information for improving rice ST by marker-assisted selection (MAS) or genome selection.

Key words: rice , whole plant growth duration salt tolerance , quantitative trait loci (QTLs) , epistasis

CHAILu1, ZHANGJian1, PANXiao-biao2, ZHANGFan1, ZHENGTian-qing1, ZHAOXiu-qing1, WANGWen-sheng1, AliJauhar3, XUJian-long1, LIZhi-kang1. Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2014, 13(8): 1609-1620.

CHAILu1 , ZHANGJian1 , PANXiao-biao2 , ZHANGFan1 , ZHENGTian-qing1 , ZHAOXiu-qing1 , WANGWen-sheng1 , AliJauhar3 , XUJian-long1 , LIZhi-kang1 . Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2014, 13(8): 1609-1620.

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Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.)

Advanced Backcross QTL Analysis for the Whole Plant Growth Duration Salt Tolerance in Rice (Oryza sativa L.)

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