Relationship Between Polyamines Metabolism and Cell Death in Roots of Malus hupehensis Rehd. Under Cadmium Stress

doi:10.1016/S1671-2927(00)8638

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (7): 1129-1136.DOI: 10.1016/S1671-2927(00)8638

Relationship Between Polyamines Metabolism and Cell Death in Roots of Malus hupehensis Rehd. Under Cadmium Stress

JIANG Qian-qian, YANG Hong-qiang, SUN Xiao-li, LI Qiang, RAN Kun, ZHANG Xin-rong

1.College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China
2.State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China

收稿日期:2011-01-03 出版日期:2012-07-01 发布日期:2012-07-27
通讯作者: YANG Hong-qiang, Tel: +86-538-8249304, E-mail: hqyang@sdau.edu.cn
作者简介:JIANG Qian-qian, Tel: +86-538-8249304, E-mail: jiangqq5238@163.com
基金资助:
This study was supported by the National Natural Science Foundation of China (30671452 and 31171923).

Relationship Between Polyamines Metabolism and Cell Death in Roots of Malus hupehensis Rehd. Under Cadmium Stress

JIANG Qian-qian, YANG Hong-qiang, SUN Xiao-li, LI Qiang, RAN Kun, ZHANG Xin-rong

1.College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, P.R.China
2.State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China

Received:2011-01-03 Online:2012-07-01 Published:2012-07-27
Contact: YANG Hong-qiang, Tel: +86-538-8249304, E-mail: hqyang@sdau.edu.cn
About author:JIANG Qian-qian, Tel: +86-538-8249304, E-mail: jiangqq5238@163.com
Supported by:
This study was supported by the National Natural Science Foundation of China (30671452 and 31171923).

摘要/Abstract

摘要： The free putrescine (Put) content, the hydrogen peroxide (H2O2) content and the polyamine oxidase (PAO) activity in roots of Malus hupehensis Rehd. var. pinyiensis Jiang (PYTC) were significantly increased, and reached its peak at 1, 2 and 6 h, respectively, under cadmium treatment. The free spermine (Spm) and spermidine (Spd) contents were dramatically decreased, and reached the minimum value at 4-6 h, then remained relatively stable. The change in total free polyamines (PAs) content was consistent with that of free Put. The number of root dead cells was gradually increased after treatment for 24 h, and the typical characteristics of programmed cell death (PCD) were displayed at 48 h. Throughout the Cd treatment process, changes in PAs metabolism appeared to be prior to cell death increase, and the H2O2 content was always maintained at a high level. These results indicated that polyamines could initiate cell death by generating H2O2 in roots of Malus hupehensis Rehd. under CdSO4 stress.

关键词: Malus hupehensis Rehd., cadmium, cell death, polyamines metabolism

Abstract: The free putrescine (Put) content, the hydrogen peroxide (H2O2) content and the polyamine oxidase (PAO) activity in roots of Malus hupehensis Rehd. var. pinyiensis Jiang (PYTC) were significantly increased, and reached its peak at 1, 2 and 6 h, respectively, under cadmium treatment. The free spermine (Spm) and spermidine (Spd) contents were dramatically decreased, and reached the minimum value at 4-6 h, then remained relatively stable. The change in total free polyamines (PAs) content was consistent with that of free Put. The number of root dead cells was gradually increased after treatment for 24 h, and the typical characteristics of programmed cell death (PCD) were displayed at 48 h. Throughout the Cd treatment process, changes in PAs metabolism appeared to be prior to cell death increase, and the H2O2 content was always maintained at a high level. These results indicated that polyamines could initiate cell death by generating H2O2 in roots of Malus hupehensis Rehd. under CdSO4 stress.

Key words: Malus hupehensis Rehd., cadmium, cell death, polyamines metabolism

JIANG Qian-qian, YANG Hong-qiang, SUN Xiao-li, LI Qiang, RAN Kun, ZHANG Xin-rong. Relationship Between Polyamines Metabolism and Cell Death in Roots of Malus hupehensis Rehd. Under Cadmium Stress[J]. Journal of Integrative Agriculture, 2012, 12(7): 1129-1136.

参考文献

[1]Apel K, Hirt H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55, 373-399.

[2]Bouchereau A, Aziz A, Larher F, Martin-Tanguy J. 1999. Polyamines and environmental challenges: recent development. Plant Science, 140, 103-125.

[3]Cheng W D, Yao H G, Zhang G P, Tang M L, Dominy P. 2005. Effect of cadmium on growth and nutrition metabolism in rice. Scientia Agricultura Sinica, 38, 528-537. (in Chinese)

[4]Collazo C, Chacón O, Borrás O. 2006. Programmed cell death in plants resembles apoptosis of animals. Biotecnologia Aplicada, 23, 1-10.

[5]Gechev T S, Hille J. 2005. Hydrogen peroxide as a signal controlling plant programmed cell death. The Journal of Cell Biology, 168, 17-20.

[6]Groppa M D, Benavides M P. 2008. Polyamines and abiotic stress: recent advances. Amino Acids, 34, 35-45.

[7]Kratsch H A, Wise R R. 2000. The ultrastructure of chilling stress. Plant, Cell and Environment, 23, 337-350.

[8]Kuehn G D. 2005. Role of polyamines in apoptosis and other recent advances in plant polyamines. Critical Reviews in Plant Sciences, 24, 123-130.

[9]Kuthanová A, Gemperlová L, Zelenková S, Eder S, Machác ková I, Opatrný Z, Cvikrová M. 2004. Cytological changes and alterations in polyamine contents induced by cadmium in tobacco BY-2 cells. Plant Physiology and Biochemistry, 42, 149-156.

[10]Lesniewska J, Simeonova E, Charzynska M. 2004. Subcellular heterogeneity of mitochondrial membrane potential in anther tapetum. Sexual Plant Reproduction, 16, 283-288.

[11]Lin A J, Zhang X H, Chen M M, Cao Q. 2007. Oxidative stress and DNA damages induced by cadmium accumulation. Journal of Environment Science, 19, 596-602.

[12]Liu L Z, Ding S Y, Tang L N. 2004. Study on Hubei wingnut (Malus hepehensis) leaf drink and it’s stability. Journal of Hubei Agricultural College, 24, 326-327. (in Chinese)

[13]Ma H Y, Yang H Q. 2006. The effect of exogenous H2O2 on mitochondrial membrane permeability and cell nuclear DNA in roots of Malus hupehensis. Journal of Physiology and Molecular Biology, 32, 551-556. (in Chinese)

[14]Maryin-Tanguy L N. 2001. Metablism and function of polyamines in plants: recent development (new approaches). Plant Growth Regulation, 34, 135-148.

[15]Peng Y Y, Peng Z S, Yu K F. 2003. The ultracultural changes of apoptotic cell. Journal of Cell Biology, 5, 280-283. (in Chinese)

[16]Politycka B, Kubi J. 2000. Changes in free polyamine level and di-and polyamine oxidase activity in cucumber roots under allelochemical stress conditions. Acta Physiologiae Plantarum, 22, 11-16.

[17]Potikha T S, Collins C C, Johnson D I, Delmer D P, Levine A. 1999. The involvement of hydrogen peroxide in the differentiation of secondary walls in cotton fibers. Plant Physiology, 119, 849-858.

[18]Ranieri A, Castagna A, Scebba F, Careri M, Zagnoni L, Predieri G, Pagliari M, di Toppi L S. 2005. Oxidative stress and phytochelatin characterization in bread wheat exposed to cadmium excess. Plant Physiology and Biochemistry, 43, 45-54.

[19]Reape T J, Molony E M, McCabe P F. 2008. Programmed cell death in plants: distinguishing between different modes. Journal of Experiment Botany, 5, 1-10.

[20]Steffens B, Sauter M. 2005. Epidermal cell death in rice is regulated by ethylene, gibberellin, and abscisic acid. Plant Physiology, 139, 713-721.

[21]Sun G W, Zhu Z J, Fang X Z. 2004. Effects of different cadmium levels on active oxygen metabolism and H2O2-scavenging system in Brassica campestris L. ssp. Chinensis. Scientia Agricultura Sinica, 37, 2012-2015. (in Chinese)

[22]Veal E A, Day A M, Morganl B A. 2007. Hydrogen peroxide sensing and signaling. Molecular Cell, 26, 1-14.

[23]Vianello A, Zancani M, Peresson C, Petrussa E, Casolo V, KrajòákováJ, Patui S, Braidot E, Macrì F. 2007. Plant mitochondrial pathway leading to programmed cell death. Physiologia Plantrum, 129, 242-252.

[24]Yoda H, Hiroi Y, Sano H. 2006. Polyamine oxidase is one of the key elements for oxidative burst to induce programmed cell death in tobacco cultured cells. Plant Physiology, 142, 193-206.

[25]Yang H Q, Shu H R. 2007. Studies on Apple Roots. Beijing Science Press, China. (in Chinese)

[26]Zhao H Z, Yang H Q. 2008. Exogenous polyamines alleviate the lipid peroxidation induced by cadmium chloride stress in Malus hupehensis Rehd. Scientia Horticulturae, 116, 442-447.

[27]Zhang J, Dong Q H, Yang K, Cao Q Q, Feng Y Q, Qin L. 2007. Studies on the ultrastructure of abnorma cell death during mutant short catkin development in chestnut. Acta Horticulturae Sinica, 34, 605-608. (in Chinese)

Relationship Between Polyamines Metabolism and Cell Death in Roots of Malus hupehensis Rehd. Under Cadmium Stress

Relationship Between Polyamines Metabolism and Cell Death in Roots of Malus hupehensis Rehd. Under Cadmium Stress

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