Fine Mapping QTLs Affecting Milk Production Traits on BTA6 in Chinese Holstein with SNP Markers

doi:10.1016/S2095-3119(13)60211-7

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (1): 110-117.DOI: 10.1016/S2095-3119(13)60211-7

Fine Mapping QTLs Affecting Milk Production Traits on BTA6 in Chinese Holstein with SNP Markers

LIU Rui, SUN Dong-xiao, WANG Ya-chun, YU Ying, ZHANG Yi, CHEN Hui-yong, ZHANG Qin, ZHANG Sheng-li , ZHANG Yuan

1.College of Animal Science and Technology/Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture/National Engineering Laboratory of Animal Genetics/China Agricultural University, Beijing 100193, P.R.China
2.Sichuan Animal Science Academy, Chengdu 610066, P.R.China

收稿日期:2011-06-08 出版日期:2013-01-01 发布日期:2013-01-24
通讯作者: Correspondence ZHANG Yuan, Tel/Fax: +86-10-62733687, E-mail: changy@cau.edu.cn
作者简介:SUN Dong-xiao, Tel: +86-10-62734653, E-mail: sundx@cau.edu.cn
基金资助:
This work was supported by the National 948 Project of China (2006-G48), the National Key Technologies R&D Program of China (2006BAD04A01), the Key Development of New Transgenic Breeds Program of China (2009ZX08009-156B), and the National Natural Science Foundation of China (31072016).

Fine Mapping QTLs Affecting Milk Production Traits on BTA6 in Chinese Holstein with SNP Markers

LIU Rui, SUN Dong-xiao, WANG Ya-chun, YU Ying, ZHANG Yi, CHEN Hui-yong, ZHANG Qin, ZHANG Sheng-li , ZHANG Yuan

1.College of Animal Science and Technology/Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture/National Engineering Laboratory of Animal Genetics/China Agricultural University, Beijing 100193, P.R.China
2.Sichuan Animal Science Academy, Chengdu 610066, P.R.China

Received:2011-06-08 Online:2013-01-01 Published:2013-01-24
Contact: Correspondence ZHANG Yuan, Tel/Fax: +86-10-62733687, E-mail: changy@cau.edu.cn
About author:SUN Dong-xiao, Tel: +86-10-62734653, E-mail: sundx@cau.edu.cn
Supported by:
This work was supported by the National 948 Project of China (2006-G48), the National Key Technologies R&D Program of China (2006BAD04A01), the Key Development of New Transgenic Breeds Program of China (2009ZX08009-156B), and the National Natural Science Foundation of China (31072016).

摘要/Abstract

摘要： Our previous studies demonstrated that the region around markers BMS470 and BMS1242 on BTA6 showed a linkage to 305-d milk yield and composition traits in the Chinese Holstein population. We herein focused on such narrow region to fine map milk production QTLs with 15 SNPs across 25 Mb with each SNP in 1 Mb within most regions in a Chinese Holstein population with daughter design. 1 449 Holstein cows and 11 sires were genotyped for such SNPs by using TaqMan probe and RFLP assays. Multipoint linkage analysis across family revealed a QTL affecting milk yield between PPARGC1A C4075T and SLC34A2 T1713C. Meanwhile, within family analysis found three milk yield QTLs (two in CR T60984131G-CEP135 C501T and one in PDLIM5 A106C-OPN T3907, a fat yield QTL in UGDH T1670C-CR T60984131G region, and two protein yield QTLs in TBC1D1 G501C-UGDH T1670C and PPARGC1A C4075T-SLC34A2 T1713C, respectively. Associations between aforementioned significant SNP markers and milk production traits were further implemented. We found significant associations of PPARGC1A C4075T, SLC34A2 T1713C with milk yield (P<0.05, P<0.01, P<0.01), UGDH T1670C, and CR T60984131G with fat yield (P<0.01, P<0.01), and PPARGC1A C4075T, SLC34A2 T1713C, UGDH T1670C and OPN T3907 with protein yield (P<0.01, P<0.01, P<0.01, P<0.01). Our findings implied that QTLs affecting milk production traits on BTA6 were pleictropism or multigenic effect and PPARGC1A and OPN may be the causal mutations behind milk production QTLs on BTA6 in the Chinese Holstein population.

关键词: fine mapping , milk production trait , SNP , BTA6 , Chinese Holstein

Abstract: Our previous studies demonstrated that the region around markers BMS470 and BMS1242 on BTA6 showed a linkage to 305-d milk yield and composition traits in the Chinese Holstein population. We herein focused on such narrow region to fine map milk production QTLs with 15 SNPs across 25 Mb with each SNP in 1 Mb within most regions in a Chinese Holstein population with daughter design. 1 449 Holstein cows and 11 sires were genotyped for such SNPs by using TaqMan probe and RFLP assays. Multipoint linkage analysis across family revealed a QTL affecting milk yield between PPARGC1A C4075T and SLC34A2 T1713C. Meanwhile, within family analysis found three milk yield QTLs (two in CR T60984131G-CEP135 C501T and one in PDLIM5 A106C-OPN T3907, a fat yield QTL in UGDH T1670C-CR T60984131G region, and two protein yield QTLs in TBC1D1 G501C-UGDH T1670C and PPARGC1A C4075T-SLC34A2 T1713C, respectively. Associations between aforementioned significant SNP markers and milk production traits were further implemented. We found significant associations of PPARGC1A C4075T, SLC34A2 T1713C with milk yield (P<0.05, P<0.01, P<0.01), UGDH T1670C, and CR T60984131G with fat yield (P<0.01, P<0.01), and PPARGC1A C4075T, SLC34A2 T1713C, UGDH T1670C and OPN T3907 with protein yield (P<0.01, P<0.01, P<0.01, P<0.01). Our findings implied that QTLs affecting milk production traits on BTA6 were pleictropism or multigenic effect and PPARGC1A and OPN may be the causal mutations behind milk production QTLs on BTA6 in the Chinese Holstein population.

Key words: fine mapping , milk production trait , SNP , BTA6 , Chinese Holstein

LIU Rui, SUN Dong-xiao, WANG Ya-chun, YU Ying, ZHANG Yi, CHEN Hui-yong, ZHANG Qin, ZHANG Sheng-li , ZHANG Yuan. Fine Mapping QTLs Affecting Milk Production Traits on BTA6 in Chinese Holstein with SNP Markers[J]. Journal of Integrative Agriculture, 2013, 12(1): 110-117.

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Fine Mapping QTLs Affecting Milk Production Traits on BTA6 in Chinese Holstein with SNP Markers

Fine Mapping QTLs Affecting Milk Production Traits on BTA6 in Chinese Holstein with SNP Markers

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