Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (10): 1949-1957.DOI: 10.1016/S2095-3119(15)61130-3

• 论文 • 上一篇    下一篇

A maize bundle sheath defective mutation mapped on chromosome 1 between SSR markers umc1395 and umc1603

 PAN  Yu, CHEN  Xu-qing, XIE  Hua, DENG  Lei, LI  Xiang-long, ZHANG  Xiao-dong, HAN  Li-xin, YANG  Feng-ping, XUE  Jing, ZHANG  Li-quan   

  1. 1、Beijing Agro-Biotechnology Research Center, Beijing Academy of Agricultural and Forestry Science, Beijing 100097, P.R.China
    2、Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education/College of Horticulture and
    Landscape Architecture, Southwest University, Chongqing 400715, P.R.China
    3、Bioengineering College, Chongqing University, Chongqing 400030, P.R.China
  • 收稿日期:2014-09-23 出版日期:2015-10-09 发布日期:2015-10-12
  • 通讯作者: CHEN Xu-qing, Tel: +86-10-51503868,Fax: +86-10-51503980, E-mail: chenxuqing@baafs.net.cn
  • 作者简介:PAN Yu, Tel: +86-23-68250974, Mobile: +86-18723494126,Fax: +86-23-68251274, E-mail: yu.pan82@yahoo.com;* These authors contributed equally to this study.
  • 基金资助:

    This research was supported by the National Natural Science Foundation of China (30700476 and 31071057) and the Beijing Natural Science Foundation, China (5083021).

A maize bundle sheath defective mutation mapped on chromosome 1 between SSR markers umc1395 and umc1603

 PAN  Yu, CHEN  Xu-qing, XIE  Hua, DENG  Lei, LI  Xiang-long, ZHANG  Xiao-dong, HAN  Li-xin, YANG  Feng-ping, XUE  Jing, ZHANG  Li-quan   

  1. 1、Beijing Agro-Biotechnology Research Center, Beijing Academy of Agricultural and Forestry Science, Beijing 100097, P.R.China
    2、Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education/College of Horticulture and
    Landscape Architecture, Southwest University, Chongqing 400715, P.R.China
    3、Bioengineering College, Chongqing University, Chongqing 400030, P.R.China
  • Received:2014-09-23 Online:2015-10-09 Published:2015-10-12
  • Contact: CHEN Xu-qing, Tel: +86-10-51503868,Fax: +86-10-51503980, E-mail: chenxuqing@baafs.net.cn
  • About author:PAN Yu, Tel: +86-23-68250974, Mobile: +86-18723494126,Fax: +86-23-68251274, E-mail: yu.pan82@yahoo.com;* These authors contributed equally to this study.
  • Supported by:

    This research was supported by the National Natural Science Foundation of China (30700476 and 31071057) and the Beijing Natural Science Foundation, China (5083021).

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摘要： The bsd-pg (bundle sheath defective pale green) mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing (SLAF-Seq) technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2:1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats (SSRs) markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.

关键词: maize , bsd-pg , SLAF , SSR assossiation analysis , fine mapping

Abstract: The bsd-pg (bundle sheath defective pale green) mutant is a novel maize mutation, controlled by a single recessive gene, which was isolated from offspring of maize plantlets regenerated from tissue callus of the maize inbred line 501. The characterization was that the biogenesis and development of the chloroplasts was mainly interfered in bundle sheath cells rather than in mesophyll cells. For mapping the bsd-pg, an F2 population was derived from a cross between the mutant bsd-pg and an inbred line Xianzao 17. Using specific locus amplified fragment sequencing (SLAF-Seq) technology, a total of 5 783 polymorphic SLAFs were analysed with 1 771 homozygous alleles between maternal and paternal parents. There were 49 SLAFs, which had a ratio of paternal to maternal alleles of 2:1 in bulked normal lines, and three trait-related candidate regions were obtained on chromosome 1 with a size of 3.945 Mb. For the fine mapping, new simple sequence repeats (SSRs) markers were designed by utilizing information of the B73 genome and the candidate regions were localized a size of 850 934 bp on chromosome 1 between umc1603 and umc1395, including 35 candidate genes. These results provide a foundation for the cloning of bsd-pg by map-based strategy, which is essential for revealing the functional differentiation and coordination of the two cell types, and helps to elucidate a comprehensive understanding of the C4 photosynthesis pathway and related processes in maize leaves.

Key words: maize , bsd-pg , SLAF , SSR assossiation analysis , fine mapping