A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP,ISSR and RAPD Markers

doi:10.1016/S1671-2927(11)60127-2

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (9): 1346-1353.DOI: 10.1016/S1671-2927(11)60127-2

A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP,ISSR and RAPD Markers

ZHANGGuang-qing, QIJian-min, ZHANGXiao-chen, FNAGPing-ping, SUJian-guang, TAOAi-fen, LANTao, WUWei-ren, LIUAi-min

1. Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops
2. Crop Sciences Institute, Fujian Academy of Agricultural Sciences
3. College of Life Science, Fujian Agriculture and Forestry University
4. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences
5. Department of Chemistry and Center for Metals in Biocatalysis, University of Minnesota

收稿日期:2010-09-02 出版日期:2011-09-01 发布日期:2011-09-09
通讯作者: Correspondence QI Jian-min, Professor, Tel: +86-591-87644898, E-mail: qijm863@163.com
作者简介:ZHANG Guang-qing, MSc, E-mail: zgq314@163.com
基金资助:
This research was supported by the National NaturalScience Fundation of China (30571188 and 31000734),the National Agri-Industry Technology Research Systemfor Crops of Bast and Leaf Fiber, China (nycytx-19-E05), and the Construction of Germplasm ResourcesPlatform for Bast Fiber Crops in Fujian, China(2010N2002).

A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP,ISSR and RAPD Markers

ZHANGGuang-qing, QIJian-min, ZHANGXiao-chen, FNAGPing-ping, SUJian-guang, TAOAi-fen, LANTao, WUWei-ren, LIUAi-min

1. Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops
2. Crop Sciences Institute, Fujian Academy of Agricultural Sciences
3. College of Life Science, Fujian Agriculture and Forestry University
4. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences
5. Department of Chemistry and Center for Metals in Biocatalysis, University of Minnesota

Received:2010-09-02 Online:2011-09-01 Published:2011-09-09
Contact: Correspondence QI Jian-min, Professor, Tel: +86-591-87644898, E-mail: qijm863@163.com
About author:ZHANG Guang-qing, MSc, E-mail: zgq314@163.com
Supported by:
This research was supported by the National NaturalScience Fundation of China (30571188 and 31000734),the National Agri-Industry Technology Research Systemfor Crops of Bast and Leaf Fiber, China (nycytx-19-E05), and the Construction of Germplasm ResourcesPlatform for Bast Fiber Crops in Fujian, China(2010N2002).

摘要/Abstract

摘要： Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. The
objective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semiwild
variety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants.
The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplified
polymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD)
markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM between
the adjacent markers.Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. Theobjective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semiwildvariety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants.The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplifiedpolymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD)markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM betweenthe adjacent markers.

关键词: kenaf, genetic map, SRAP, ISSR, RAPD

Abstract: Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. The
objective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semiwild
variety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants.
The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplified
polymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD)
markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM between
the adjacent markers.Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for non-wood fiber production. Theobjective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semiwildvariety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants.The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplifiedpolymorphism (SRAP), 56 inter-simple sequence repeat (ISSR), and 13 randomly amplified polymorphic DNA (RAPD)markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM betweenthe adjacent markers.

Key words: kenaf, genetic map, SRAP, ISSR, RAPD

ZHANGGuang-qing, QIJian-min, ZHANGXiao-chen, FNAGPing-ping, SUJian-guang, TAOAi-fen, LANTao, WUWei-ren, LIUAi-min. A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP,ISSR and RAPD Markers[J]. Journal of Integrative Agriculture, 2011, 10(9): 1346-1353.

ZHANGGuang-qing , QIJian-min , ZHANGXiao-chen , FNAGPing-ping , SUJian-guang , TAOAi-fen , LANTao , WUWei-ren , LIUAi-min . A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP,ISSR and RAPD Markers[J]. Journal of Integrative Agriculture, 2011, 10(9): 1346-1353.

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A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP,ISSR and RAPD Markers

A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP,ISSR and RAPD Markers

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