Effect of Cadmium Stress on the Growth, Antioxidative Enzymes and Lipid Peroxidation in Two Kenaf (Hibiscus cannabinus L.) Plant Seedlings

doi:10.1016/S2095-3119(13)60279-8

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (4): 610-620.DOI: 10.1016/S2095-3119(13)60279-8

Effect of Cadmium Stress on the Growth, Antioxidative Enzymes and Lipid Peroxidation in Two Kenaf (Hibiscus cannabinus L.) Plant Seedlings

LI Feng-tao, QI Jian-min, ZHANG Gao-yang, LIN Li-hui, FANG Ping-ping, TAO Ai-fen , XU Jian-tang

Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China

收稿日期:2012-08-20 出版日期:2013-04-01 发布日期:2013-04-07
通讯作者: Correspondence QI Jian-min, Tel/Fax: +86-591-83789420, E-mail: qijm863@163.com
基金资助:
Acknowledgements This study was funded by the Project of Fiber Crops Industrial System Construction in China (CARS-19-E06), the Project for Constructing Observations Station of Scientific Experiment of Jute and Kenaf in the South East of China, Ministry of Agriculture of China (2011.9), and the Construction of Germplasm Resources Platform for Bast Fiber Crops in Fujian, China (2010N2002).

Effect of Cadmium Stress on the Growth, Antioxidative Enzymes and Lipid Peroxidation in Two Kenaf (Hibiscus cannabinus L.) Plant Seedlings

LI Feng-tao, QI Jian-min, ZHANG Gao-yang, LIN Li-hui, FANG Ping-ping, TAO Ai-fen , XU Jian-tang

Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China

Received:2012-08-20 Online:2013-04-01 Published:2013-04-07
Contact: Correspondence QI Jian-min, Tel/Fax: +86-591-83789420, E-mail: qijm863@163.com
Supported by:
Acknowledgements This study was funded by the Project of Fiber Crops Industrial System Construction in China (CARS-19-E06), the Project for Constructing Observations Station of Scientific Experiment of Jute and Kenaf in the South East of China, Ministry of Agriculture of China (2011.9), and the Construction of Germplasm Resources Platform for Bast Fiber Crops in Fujian, China (2010N2002).

摘要/Abstract

摘要： The effects of cadmium stress on the growth, antioxidative enzymes and lipid peroxidation in two kenaf plants, Fuhong 991 and ZM412, were analysed under control (0.5-strength Hoagland’s nutrient solution) or five levels of cadmium stress (0.5- strength Hoagland’s nutrient solution containing different concentrations of Cd2+). The leaves and roots of control and cadmium-stressed plants were harvested after 3 wk. At the same Cd concentration, the Cd tolerance index of Fuhong 991 was higher than that of ZM412, indicating that Fuhong 991 may be more tolerant to Cd than ZM412. The superoxide dismutase (SOD), catalase activity (CAT) and peroxidase (POD) activities fluctuated in the leaves of the Cd-stressed plants compared to the control, whereas the glutathione reductase activity (GR) was much larger than the control for Fuhong 991, ensuring that sufficient quantities of GSH were available to respond to the cadmium stress. In comparison to the control, the dynamic tendency of the SOD, CAT and POD activities in roots of the Cd-stressed plants all increased and then declined, but the POD activity of Fuhong 991 remained nearly unchanged at all of the stress levels. The increase in the enzyme activities demonstrated that Fuhong 991 was more tolerant to cadmium than ZM 412. The lipid peroxidation was enhanced only in the leaves of Cd-stressed ZM 412. These findings indicated that antioxidative activities may play important roles in Cd-stressed Fuhong 991 and ZM 412 and that the leaf and root cell membranes of Fuhong 991 have a greater stability than those of ZM 412. For pollution monitoring purposes, the GR activity in the roots and leaves may serve as a biomarker of Cd for Fuhong 991, whereas lipid peroxidation may serve as biomarker for ZM 412.

关键词: kenaf , cadmium stress , superoxide dismutase (SOD) , catalase (CAT) , peroxidase (POD) , glutathione reductase (GR) , glutathione (GSH)

Abstract: The effects of cadmium stress on the growth, antioxidative enzymes and lipid peroxidation in two kenaf plants, Fuhong 991 and ZM412, were analysed under control (0.5-strength Hoagland’s nutrient solution) or five levels of cadmium stress (0.5- strength Hoagland’s nutrient solution containing different concentrations of Cd2+). The leaves and roots of control and cadmium-stressed plants were harvested after 3 wk. At the same Cd concentration, the Cd tolerance index of Fuhong 991 was higher than that of ZM412, indicating that Fuhong 991 may be more tolerant to Cd than ZM412. The superoxide dismutase (SOD), catalase activity (CAT) and peroxidase (POD) activities fluctuated in the leaves of the Cd-stressed plants compared to the control, whereas the glutathione reductase activity (GR) was much larger than the control for Fuhong 991, ensuring that sufficient quantities of GSH were available to respond to the cadmium stress. In comparison to the control, the dynamic tendency of the SOD, CAT and POD activities in roots of the Cd-stressed plants all increased and then declined, but the POD activity of Fuhong 991 remained nearly unchanged at all of the stress levels. The increase in the enzyme activities demonstrated that Fuhong 991 was more tolerant to cadmium than ZM 412. The lipid peroxidation was enhanced only in the leaves of Cd-stressed ZM 412. These findings indicated that antioxidative activities may play important roles in Cd-stressed Fuhong 991 and ZM 412 and that the leaf and root cell membranes of Fuhong 991 have a greater stability than those of ZM 412. For pollution monitoring purposes, the GR activity in the roots and leaves may serve as a biomarker of Cd for Fuhong 991, whereas lipid peroxidation may serve as biomarker for ZM 412.

Key words: kenaf , cadmium stress , superoxide dismutase (SOD) , catalase (CAT) , peroxidase (POD) , glutathione reductase (GR) , glutathione (GSH)

LI Feng-tao, QI Jian-min, ZHANG Gao-yang, LIN Li-hui, FANG Ping-ping, TAO Ai-fen , XU Jian-tang. Effect of Cadmium Stress on the Growth, Antioxidative Enzymes and Lipid Peroxidation in Two Kenaf (Hibiscus cannabinus L.) Plant Seedlings[J]. Journal of Integrative Agriculture, 2013, 12(4): 610-620.

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Effect of Cadmium Stress on the Growth, Antioxidative Enzymes and Lipid Peroxidation in Two Kenaf (Hibiscus cannabinus L.) Plant Seedlings

Effect of Cadmium Stress on the Growth, Antioxidative Enzymes and Lipid Peroxidation in Two Kenaf (Hibiscus cannabinus L.) Plant Seedlings

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