Transcriptomic Analysis of the Highly Heterotic Maize Hybrid Zhengdan 958 and Its Parents During Spikelet and Floscule Differentiation

doi:10.1016/S1671-2927(00)8713

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (11): 1783-1793.DOI: 10.1016/S1671-2927(00)8713

Transcriptomic Analysis of the Highly Heterotic Maize Hybrid Zhengdan 958 and Its Parents During Spikelet and Floscule Differentiation

LI Zhi-yong, ZHANG Ti-fu, WANG Shou-cai

National Maize Improvement Center/Maize Breeding Engineering Center, Ministry of Education/Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P.R.China

收稿日期:2011-07-26 出版日期:2012-11-01 发布日期:2012-11-24
通讯作者: Correspondence WANG Shou-cai, Tel/Fax: +86-10-62732409, E-mail: wangshoucai678@sina.com
基金资助:
This work was supported by the National High-Tech R&D Program of China (2011AA10A103) and the National Basic Research Program of China (2009CB118400).

Transcriptomic Analysis of the Highly Heterotic Maize Hybrid Zhengdan 958 and Its Parents During Spikelet and Floscule Differentiation

LI Zhi-yong, ZHANG Ti-fu, WANG Shou-cai

National Maize Improvement Center/Maize Breeding Engineering Center, Ministry of Education/Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P.R.China

Received:2011-07-26 Online:2012-11-01 Published:2012-11-24
Contact: Correspondence WANG Shou-cai, Tel/Fax: +86-10-62732409, E-mail: wangshoucai678@sina.com
Supported by:
This work was supported by the National High-Tech R&D Program of China (2011AA10A103) and the National Basic Research Program of China (2009CB118400).

摘要/Abstract

摘要： Heterosis plays an important role in crop production and plant evolution. Although heterosis has been widely exploited by plant breeders, the underlying molecular mechanisms are not well understood. We analyzed gene expression of the highly heterotic maize hybrid Zhengdan 958 and its parents, Zheng 58 and Chang 7-2 during spikelet and floscule differentiation using the GeneChip® Maize Genome Array. Pairwise comparison among Zhengdan 958 and its parents at the two stages of immature ear development identfied 1 089 and 1 352 differentially expressed genes. Gene ontology (GO) functional analysis showed that these genes participate in many functional categories, and those encoding response to stress and transcription factor may play important roles in heterosis. Pathway analysis showed that the differentially expressed genes are involved in various metabolic processes, and those participating in lipid metabolism, signal transduction, transport, and catabolism may contribute to heterosis. A non-additive expression pattern was prevalent in genes that were differentially expressed between the hybrid and its parents during both spikelet and floscule differentiation. Because genes that are differentially expressed in a hybrid and its parents could underlie heterosis, nonadditive expression patterns might contribute to the manifestation of heterosis.

关键词: maize, heterosis, microarray, gene expression, Zhengdan 958, spikelet, floscule

Abstract: Heterosis plays an important role in crop production and plant evolution. Although heterosis has been widely exploited by plant breeders, the underlying molecular mechanisms are not well understood. We analyzed gene expression of the highly heterotic maize hybrid Zhengdan 958 and its parents, Zheng 58 and Chang 7-2 during spikelet and floscule differentiation using the GeneChip® Maize Genome Array. Pairwise comparison among Zhengdan 958 and its parents at the two stages of immature ear development identfied 1 089 and 1 352 differentially expressed genes. Gene ontology (GO) functional analysis showed that these genes participate in many functional categories, and those encoding response to stress and transcription factor may play important roles in heterosis. Pathway analysis showed that the differentially expressed genes are involved in various metabolic processes, and those participating in lipid metabolism, signal transduction, transport, and catabolism may contribute to heterosis. A non-additive expression pattern was prevalent in genes that were differentially expressed between the hybrid and its parents during both spikelet and floscule differentiation. Because genes that are differentially expressed in a hybrid and its parents could underlie heterosis, nonadditive expression patterns might contribute to the manifestation of heterosis.

Key words: maize, heterosis, microarray, gene expression, Zhengdan 958, spikelet, floscule

LI Zhi-yong, ZHANG Ti-fu, WANG Shou-cai. Transcriptomic Analysis of the Highly Heterotic Maize Hybrid Zhengdan 958 and Its Parents During Spikelet and Floscule Differentiation[J]. Journal of Integrative Agriculture, 2012, 12(11): 1783-1793.

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Transcriptomic Analysis of the Highly Heterotic Maize Hybrid Zhengdan 958 and Its Parents During Spikelet and Floscule Differentiation

Transcriptomic Analysis of the Highly Heterotic Maize Hybrid Zhengdan 958 and Its Parents During Spikelet and Floscule Differentiation

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