杨柳田头菇YAAS711菌株F1子代胞外酶活性分析

doi:10.3864/j.issn.0578-1752.2019.20.017

摘要/Abstract

摘要：

【目的】 探究杨柳田头菇YAAS711菌株的单孢菌株及其自交个体的胞外酶活性变化规律,为其遗传育种提供理论依据。【方法】 以杨柳田头菇YAAS711的18个单核体与22个自交双核体为试验材料,分别采用3, 5-二硝基水杨酸（DNS）法、福林法、愈创木酚法等测定羧甲基纤维素酶、滤纸纤维素酶、β-葡萄糖苷酶、半纤维素酶、淀粉酶、蛋白酶、漆酶、多酚氧化酶、过氧化物酶9种胞外酶活性,并对这9种胞外酶与子代生长速度、极性和交配因子进行相关性分析。【结果】 单核体和双核体绝大多数都具有较高的淀粉酶、滤纸纤维素酶、半纤维素酶、β-葡萄糖苷酶、羧甲基纤维素酶及蛋白酶活性,过氧化物酶、多酚氧化酶及漆酶活性却较低。9种胞外酶平均活力与单核体和双核体的菌丝生长速度均存在一定程度的相关性,除部分类型Ⅱ单核体外,菌丝生长速度较快的单核体具有较高的胞外酶活性;反之,胞外酶活性较低。相比单核体菌丝生长速度“快-快”的组合,单核体菌丝生长速度“快-慢”的组合除半纤维素酶、蛋白酶和多酚氧化酶外,其他胞外酶活性都较高。具有类型Ⅱ单核体参与形成的双合体胞外酶活性却各不相同且无规律。多酚氧化酶对单核体菌丝生长速度的间接影响大于漆酶,但漆酶对单核体菌丝生长速度的综合作用最大;胞外酶之间的协同作用对双核体菌丝生长速度影响较小,其中漆酶对其菌丝生长速度直接作用最大。多重比较结果显示,在显著水平为0.05上,极性对胞外酶平均活力的影响大于交配因子。【结论】 单核体菌丝生长速度与本极性异常和杨柳田头菇YAAS711子代个体胞外酶活性差异存在显著相关性,除部分类型Ⅱ单核体和双核体外,类型Ⅰ菌株胞外酶活性具有相同的规律。单核体菌丝生长速度、极性、胞外酶三者之间具有一定的相关性,但交配因子与胞外酶没有直接相关性。

关键词: 杨柳田头菇, 自交后代, 胞外酶活性, 单核体, 双核体

Abstract:

【Objective】 The objective of this study was to explore the changes in activities of extracellular enzymes (EEA) of single-spore isolations and its self-crossing individuals from Agrocybe salicacola YAAS711 and to provide evidence for its genetic breeding. 【Method】 Eighteen single-spore monokaryons and 22 dikaryons from self-crossing progeny of A. salicacola YAAS711 were used as the test materials. The activities of CMCase, filter paper cellulase, β-glucanase, hemicellulase, amylase, protease, laccase, polyphenolase and peroxidase of all test strains were determined by 3,5-dinitrosalicylic acid (DNS), Folin methods and guaiacol method, and correlation analysis were carried out between the 9 EEs with mycelial growth rate, polarities and mating factors.【Results】 The results of 9 EEs showed that most of the both monokaryons and dikaryons had higher activities of amylase, filter paper cellulase, hemicellulose, β-glucanase, CMCase and protease, but their activities of peroxidase, polyphenolase and laccase were low. The mycelial growth rate of monokaryons and dikaryons had a certain degree of correlation with the average activity of 9 EEAs. Compared with the dikaryons derived from the combination of “fast-fast” growth rate of monokaryons, the dikaryons derived from the combination of “fast-slow” growth rates of monokaryons had higher EEAs, except hemicellulase, protease and polyphenol oxidase activities. However, there were no rules to follow, when it came to the combination of dikaryons from the cross of type II monokaryons. The indirect effect of polyphenolase on the mycelial growth rate of monokaryons was greater than that of laccase, but the combined effect of laccase on the mycelial growth rate of monokaryons was the largest. The synergistic effect among EEs had little effect on the mycelial growth rate of dikaryons, among them, laccase had the greatest direct effect on its mycelial growth rate. Multiple comparisons showed that the effect of polarity on EEs was greater than that of mating factors at a significant level of 0.05.【Conclusion】 In short, there was a significant correlation between the anomalies of the mycelial growth rate of the monokaryons from same polarity single spore isolations and the EEAs of F1 generations deriverd from these monokaryons in A. salicacola strain YAAS711. The EEAs of type I strains had same rule, but the differences were existed in some type II monokaryons and the dikaryons. There were certain correlation between the growth rate of monokaryons, EEAs and polarity, there were no direct correlation between mating factors and EEAs.

Key words: Agrocybe salicacola, self-crossing F₁ generations, extracellular enzyme activity, monokaryon, dikaryon

周会明,赵永昌,柴红梅,张焱珍. 杨柳田头菇YAAS711菌株F₁子代胞外酶活性分析[J]. 中国农业科学, 2019, 52(20): 3672-3684.

HuiMing ZHOU,YongChang ZHAO,HongMei CHAI,YanZhen ZHANG. The Extracellular Enzymes Activity of F₁ Generations and Single-spore Isolations in Agrocybe salicacola Strain YAAS711[J]. Scientia Agricultura Sinica, 2019, 52(20): 3672-3684.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2019.20.017

https://www.chinaagrisci.com/CN/Y2019/V52/I20/3672

图/表 9

表1

图1

表2

杨柳田头菇不同单核体的菌丝生长速度与胞外酶平均活力的差异"

单核体 Monokaryon	菌丝生长速度 Mycelial growth rate (cm?d^-1)	胞外酶平均活力 Average activity of extracellular enzymes (U)
单核体 Monokaryon	菌丝生长速度 Mycelial growth rate (cm?d^-1)	CMC	FP	βG	HC	AM	PR	LA	PPO	POD
001	0.30±0.01c	3.20±0.68a	3.89±0.60bcdef	1.55±0.35cde	2.77±0.88bcdef	7.59±0.86a	1.48±0.33abcd	0.77±0.04b	0.27±0.06fg	0.39±0.06a
020	0.39±0.01a	0.10±0.03f	3.43±0.41cdefgh	1.42±0.21cde	3.41±0.37ab	6.28±0.13b	1.21±0.03abcde	0.70±0.02c	0.58±0.03b	0.19±0.03d
026	0.15±0.00e	2.68±0.58ab	3.20±0.46defgh	2.11±0.58bcd	2.57±0.53cdefg	7.80±0.08a	1.50±0.55abc	0.13±0.01fgh	0.34±0.08ef	0.31±0.05ab
029	0.14±0.02e	1.28±0.19de	5.23±1.28ab	1.73±0.23cde	3.04±0.28abcde	2.02±0.26e	0.89±0.11cde	0.07±0.01h	0.10±0.01jk	0.35±0.07a
032	0.34±0.03b	1.92±0.90bcd	4.67±0.12bcd	3.17±1.77ab	3.00±0.38abcde	5.47±0.19c	1.22±0.11abcde	0.69±0.02c	0.61±0.07ab	0.21±0.05cd
034	0.23±0.01d	2.34±0.57abc	2.74±0.67fgh	1.75±0.15cd	2.59±0.47cdefg	4.21±0.43d	1.22±0.18abcde	0.18±0.07ef	0.18±0.05hi	0.36±0.04a
036	0.33±0.01b	2.30±0.56abc	4.42±0.85bcde	3.09±0.73ab	2.40±0.05defg	4.27±0.30d	1.27±0.18abcde	0.06±0.01h	0.42±0.08d	0.31±0.02ab
048	0.15±0.01e	0.15±0.04f	3.54±1.41cdefg	1.48±0.22cde	3.13±0.14abcd	0.95±0.40f	0.81±0.01e	0.15±0.02efg	0.21±0.04ghi	0.35±0.03a
071	0.14±0.01e	2.29±0.12abc	4.75±0.82bc	0.42±0.19e	1.89±0.20g	3.66±0.73d	1.34±0.32abcde	0.11±0.01fgh	0.15±0.02ij	0.35±0.08a
076	0.11±0.01f	0.66±0.56ef	6.18±0.85a	1.35±0.84cde	2.24±0.16efg	1.22±0.50f	0.81±0.04e	0.08±0.01gh	0.23±0.02gh	0.32±0.03ab
096	0.33±0.01b	2.56±0.41ab	1.99±0.83h	3.46±0.86a	2.59±0.69cdefg	5.36±0.12c	1.73±0.85ab	0.87±0.02a	0.31±0.04f	0.36±0.02a
130	0.15±0.01e	1.15±0.56de	3.85±0.44bcdef	1.28±0.52de	3.04±0.20abcde	2.11±0.40e	0.84±0.05de	0.39±0.08d	0.04±0.01k	0.31±0.06ab
163	0.15±0.00e	2.27±0.33abc	2.36±0.97gh	1.65±0.77cde	3.21±0.74abc	0.83±0.35f	0.90±0.07cde	0.11±0.09fgh	0.14±0.03ij	0.34±0.06a
173	0.15±0.00e	2.42±0.20abc	3.16±0.50efgh	1.57±0.95cde	3.62±0.32a	7.66±0.54a	1.16±0.43abcde	0.15±0.01efg	0.50±0.03c	0.30±0.08abc
182	0.13±0.01e	2.64±0.25abc	4.78±0.74bc	2.61±0.37abc	3.66±0.16a	5.12±0.58c	1.13±0.24bcde	0.15±0.01efg	0.40±0.02de	0.34±0.01a
185	0.15±0.00e	0.64±0.07ef	3.46±0.17cdefg	0.91±0.55de	2.02±0.35fg	0.90±0.43f	0.94±0.01cde	0.22±0.07e	0.27±0.03fg	0.31±0.06ab
186	0.35±0.03b	1.53±0.58cde	4.59±0.16bcde	1.52±0.21cde	2.41±0.13cdefg	4.37±0.11d	1.79±0.61a	0.65±0.02c	0.66±0.03a	0.34±0.08a
187	0.15±0.00e	1.23±0.61de	3.54±0.94cdefg	1.45±0.36cde	3.04±0.07abcde	1.96±0.51e	0.88±0.11cde	0.10±0.02gh	0.23±0.01gh	0.32±0.03bcd

表2

表3

杨柳田头菇不同双核体的菌丝生长速度与胞外酶平均活力的差异"

双核体 Dikaryon	菌丝生长速度 Mycelial growth rate (cm?d^-1)	胞外酶平均活力 Average activity of extracellular enzymes (U)
双核体 Dikaryon	菌丝生长速度 Mycelial growth rate (cm?d^-1)	CMC	FP	βG	HC	AM	PR	LA	PPO	POD
001*020	0.29±0.01g	2.96±0.22bcde	5.13±0.68bcde	3.07±0.22ab	1.61±0.51de	6.00±0.72cdef	1.33±0.26abcd	0.87±0.02b	0.47±0.03cd	0.28±0.02def
001*029	0.32±0.02def	3.53±0.10abcd	4.30±1.21cdefg	3.22±0.93ab	3.31±0.72a	10.09±0.97a	1.18±0.21abcd	1.26±0.09a	0.26±0.02gh	0.48±0.04a
001*032	0.30±0.01fg	0.88±0.64h	4.87±0.20cdef	3.13±0.25ab	3.09±0.40a	6.83±0.31bcd	1.50±0.05abcd	0.74±0.22b	0.42±0.07cde	0.27±0.10def
001*071	0.30±0.03fg	3.85±0.26abc	5.02±0.20bcdef	3.18±0.88ab	3.29±0.55a	6.77±0.40bcd	1.03±0.67bcd	1.36±0.43a	0.23±0.05hi	0.45±0.10ab
001*187	0.35±0.00abc	3.52±0.44abcd	4.25±0.52cdefg	3.23±0.72ab	3.28±0.50a	6.90±0.84bc	1.29±0.30abcd	1.30±0.03a	0.20±0.04hi	0.34±0.03cde
020*130	0.34±0.00bcd	4.54±0.79a	3.44±0.75efgh	1.58±0.42def	1.35±0.36ef	7.85±0.27b	1.62±0.28abcd	1.13±0.03a	0.35±0.11efg	0.27±0.10def
020*173	0.36±0.02ab	2.96±0.51bcde	4.52±0.59cdefg	2.70±0.51bcd	2.95±0.34abc	9.38±0.20a	1.48±0.40abcd	1.22±0.18a	0.27±0.04gh	0.25±0.02defg
020*185	0.33±0.01bcde	4.56±0.66a	6.60±1.24b	3.19±0.60ab	0.45±0.09f	4.98±0.49fgh	1.28±0.31abcd	1.13±0.01a	0.49±0.11c	0.18±0.03fgh
032*130	0.37±0.02a	3.50±0.70abcd	5.98±0.51bc	3.02±0.61abc	1.87±0.59bcde	7.00±0.16bc	1.64±0.93abc	1.23±0.04a	0.28±0.05fgh	0.23±0.04efgh
032*173	0.35±0.01abc	3.00±0.92bcde	4.75±0.44cdefg	3.40±0.87ab	0.88±0.24ef	7.00±0.92bc	1.18±0.36abcd	1.27±0.19a	0.35±0.02efg	0.14±0.01h
032*185	0.35±0.01abc	4.06±0.12ab	2.44±0.93h	1.78±0.16cdef	1.87±0.50cde	5.56±0.55defg	1.52±0.10abcd	1.23±0.02a	0.19±0.02hi	0.24±0.03defgh
071*130	0.22±0.00h	2.63±0.52cde	4.43±0.33cdefg	0.78±0.77f	1.56±0.28de	3.42±0.10i	0.95±0.03cd	0.10±0.01c	0.67±0.04ab	0.32±0.06cde
071*173	0.21±0.01hi	1.49±0.46gh	3.42±0.65efgh	1.43±0.42ef	2.68±0.91abcd	1.23±0.34j	0.86±0.05d	0.06±0.02c	0.59±0.03b	0.35±0.03bcd
071*185	0.21±0.01hi	2.09±0.81ef	4.64±0.84cdefg	0.74±0.09f	1.94±0.25bcde	7.35±0.48bc	0.99±0.17cd	0.09±0.02c	0.74±0.07a	0.35±0.07bcd
036*096	0.23±0.01h	2.61±0.73de	3.96±0.73defgh	2.90±0.39abc	1.99±0.91bcde	4.18±0.53hi	1.89±0.73a	0.70±0.01b	0.38±0.05def	0.16±0.03gh
036*186	0.19±0.01i	2.71±0.30cde	3.33±0.61fgh	2.89±0.87abc	1.52±0.47e	4.63±0.63ghi	1.79±0.10ab	0.71±0.04b	0.38±0.11def	0.28±0.01def
096*026	0.31±0.01efg	3.21±0.71bcde	4.31±0.51cdefg	1.13±0.72ef	1.91±0.96bcde	6.19±0.65cdef	1.16±0.37abcd	1.20±0.06a	0.35±0.04efg	0.14±0.10h
096*034	0.29±0.02g	3.14±1.05bcde	3.04±0.66gh	2.82±0.90abc	3.57±0.08a	4.68±0.56gh	1.70±0.09abc	1.34±0.27a	0.38±0.02def	0.25±0.01defg
096*048	0.30±0.01fg	2.91±0.68bcde	4.64±1.01cdefg	2.30±0.96bcde	2.98±0.67ab	7.15±1.34bc	1.29±0.30abcd	1.21±0.19a	0.22±0.03hi	0.41±0.01abc
096*076	0.31±0.01fg	3.48±0.85abcd	4.19±2.25defg	4.05±0.52a	1.93±0.22bcde	7.26±1.53bc	1.47±0.11abcd	1.22±0.01a	0.14±0.02i	0.31±0.10cde
096*163	0.33±0.03cde	2.84±0.22bcde	8.30±1.48a	3.17±0.44ab	1.99±0.82bcde	6.42±0.79cde	1.35±0.37abcd	1.22±0.08a	0.25±0.09gh	0.41±0.05abc
096*182	0.29±0.00g	3.44±0.70abcd	5.36±0.70bcd	2.86±0.88abc	3.14±0.94a	5.37±0.62efgh	1.39±0.57abcd	1.21±0.06a	0.29±0.02fgh	0.24±0.01defgh

表3

Table 4

表5

表6

表7

表8

参考文献 51

[1]	杨祝良, 臧穆, 刘学系 . 杨柳田头菇—无孔组的一个滇产新种. 云南植物研究, 1993,15(1):18-20.
	YANG Z L, ZANG M, LIU X X . Agrocybe salicacola, a new species of sect. aporus from Yunnan, Acta Botanica Yunnanica, 1993,15(1):18-20. (in Chinese)
[2]	FAYOD V . Prodrome d'une histoire naturelle des Agaricines. Annales des Sciences Naturelles, Botanique VII, 1889,7(9):181-411.
[3]	臧穆, 杨祝良, 刘学系 . 一种滇产的美味新食菌杨柳田头菇. 中国食用菌, 1991,13(6):8.
	ZANG M, YANG Z L, LIU X X . Agrocybe salicacola, a delicious edible mushroom newly discove red from Yunnan. Edible Fungi of China, 1991,13(6):8. (in Chinese)
[4]	金鑫 . 中国广义球盖菇科几个属的分类学研究[D]. 长春: 吉林农业大学, 2012.
	JIN X . Studies on taxonomy of several genera of strophariaceae (s. l.) from China[D]. Changchun: Jilin Agricultural University, 2012. ( in Chinese)
[5]	周会明 . 杨柳田头菇生活史及分类地位研究[D]. 昆明: 昆明理工大学, 2011.
	ZHOU H M . Studies on the life cycle and classification of Agrocybe salicacola[D]. Kunming: Kunming University of Science and Technology, 2011. ( in Chinese)
[6]	汪欣, 刘平 . 杨柳田头菇引种驯化试验研究. 中国食用菌, 2004,23(2):16-17.
	WANG X, LIU P . Studies on domestication of Agrocybe salicacola. Edible Fungi of China, 2004,23(2):16-17. (in Chinese)
[7]	LIU L Y, LI Z H, DONG Z J, LI X Y, SU J, LI Y, LIU J K . Two novel fomannosane-type sesquiterpenoids from the culture of the basidiomycete Agrocybe salicacola. Natural Products and Bioprospecting, 2012,2(3):130-132.
[8]	LIU L Y, ZHANG L, FENG T, LI Z H, DONG Z J, LI X Y, LIU J K . Unusual illudin-type sesquiterpenoids from cultures of Agrocybe salicacola. Natural Products and Bioprospecting, 2011,1(2):87-92.
[9]	ZHU Y C, WANG G, YANG X L, LUO D Q, ZHU Q C, PENG T, LIU J K . Agrocybone, a novel bis-sesquiterpene with a spirodienone structure from basidiomycete Agrocybe salicacola. Tetrahedron Letters, 2010,51(26):3443-3445.
[10]	CHEN W M, ZHANG X L, CHAI H M, CHEN L J, LIU W L, ZHAO Y C . Comparative analysis of aporulating and spore-deficient strains of Agrocybe salicacola based on the transcriptome sequences. Current Microbiology, 2015,71(2):204-213.
[11]	CHEN W M, CHAI H M, YANG W X, ZHANG X L, CHEN Y H, ZHAO Y C . Characterization of non-coding regions in B mating loci of Agrocybe salicacola groups: target sites for B mating type identification. Current Microbiology, 2017,74(6):772-778.
[12]	周会明, 赵永昌, 陈卫民, 柴红梅, 李树红, 赵静 . 杨柳田头菇交配型因子与菌丝生长速度关系. 云南植物研究, 2010,32(4):315-322.
	ZHOU H M, ZHAO Y C, CHEN W M, CHAI H M, LI S H, ZHAO J . The relationship between mating factors with mycelia growth rate of Agrocybe salicacola (Bolbitiaceae). Acta Botanica Yunnanica, 2010,32(4):315-322. (in Chinese)
[13]	周会明, 张小雷, 马美芳, 杨丽芬, 赵永昌 . 田头菇属不同分离菌株杂交研究. 生物技术, 2011,21(1):69-73.
	ZHOU H M, ZHANG X L, MA M F, YANG L F, ZHAO Y C . Study on the hybridization for different isolates of genus Agrocybe. Biotechnology, 2011,21(1):69-73. (in Chinese)
[14]	曹春蕾, 崔宝凯, 秦问敏 . 桑木层孔菌液体培养过程中几种胞外酶活性的变化. 菌物学报, 2011,30(2):275-280.
	CAO C L, CUI B K, QIN W M . Activity changes of several extracellular enzymes in liquid culture of Phellinus mori. Mycosystema, 2011,30(2):275-280. (in Chinese)
[15]	方宏阳 . 不同颜色的毛木耳菌株生长发育过程及胞外酶活性研究[D]. 长春: 吉林农业大学, 2017.
	FANG H Y . Study on the growth and development and extracellular enzyme activity of different color strain of Auricularia cornea[D]. Changchun: Jilin Agricultural University, 2017. ( in Chinese)
[16]	刘兵 . γ-氨基丁酸和海藻糖对高温胁迫香菇菌丝胞外酶活性的影响[D]. 晋中: 山西农业大学, 2017.
	LIU B . Effect of GABA and trehalose on the activity of exoenzyme induced by heat stress in mycelia of Lentinus edodes[D]. Jinzhong: Shanxi Agricultural University, 2017. ( in Chinese)
[17]	ZERVA A, ZERVAKIS G I, CHRISTAKOPOULOS P, TOPAKAS E . Degradation of olive mill wastewater by the induced extracellular ligninolytic enzymes of two wood-rot fungi. Journal of Environmental Management, 2017,203(2):791-798.
[18]	HU C J, HUANG D L, ZENG G M, CHENG M, GONG X M, WANG R Z, XUE W J, HU Z X, LIU Y N . The combination of Fenton process and Phanerochaete chrysosporium for the removal of bisphenol A in river sediments: Mechanism related to extracellular enzyme, organic acid and iron. Chemical Engineering Journal, 2018,338:432-439.
[19]	PANDEY R K, TEWARI S, TEWARI L . Lignolytic mushroom Lenzites elegans WDP2: Laccase production, characterization, and bioremediation of synthetic dyes. Ecotoxicology and Environmental Safety, 2018,158:50-58.
[20]	ZHAI F H, HAN J R . Decomposition of asparagus old stalks by Pleurotus spp. under mushroom-growing conditions. Scientia Horticulturae, 2018,231:11-14.
[21]	郝敬喆 . 新疆野生巴尔喀什蘑菇驯化栽培与漆酶活性研究[D]. 北京: 中国农业大学, 2019.
	HAO J Z . Domesticated cultivation of Xinjiang wild mushroom Agaricus balchaschensis and laccase activity[D]. Beijing: China Agricultural University, 2019. ( in Chinese)
[22]	曾春函, 王传华 . 我国主要蜜环菌生物种胞外酶活性对温度的种特异性响应. 菌物学报, 2019,38(1):1-15.
	ZENG C H, WANG C H . Specificity response of extracellular enzyme activities to temperature of eight Armillaria species in China. Mycosystema, 2019,38(1):1-15. (in Chinese)
[23]	方宏阳, 任梓铭, 孟秀秀, 代俊杰, 李寿建, 黎志文, 李晓 . 玉木耳胞外酶变化与农艺性状关系. 分子植物育种, 2018,16(13):4431-4435.
	FANG H Y, REN Z M, MENG X X, DAI J J, LI S J, LI Z W, LI X . The relationship between extracellular enzymes changes and agronomic traits in Auricularia fuscosuccinea. Molecular Plant Breeding, 2018,16(13):4431-4435. (in Chinese)
[24]	李利梅, 朱国胜, 黄万兵, 卢颖颖, 龚光禄, 张丽娜, 杨通静, 桂阳 . 红托竹荪母种菌丝生长特性及胞外酶活性. 食用菌学报, 2018,25(3):30-35.
	LI L M, ZHU G S, HUANG W B, LU Y Y, GONG G L, ZHANG L N, YANG T J, GUI Y . Mycelial growth characteristics and extracellular enzyme activity of Dictyophora rubrovalvata. Acta Edulis Fungi, 2018,25(3):30-35. (in Chinese)
[25]	吴亚召, 张文隽, 雷萍, 杜芳 . 茶树菇生长发育过程中几种胞外酶活性的变化. 西北农林科技大学学报(自然科学版), 2018,46(11):115-120.
	WU Y Z, ZHANG W J, LEI P, DU F . Changes of extracellular enzymes activities during development of Agrocybe aegerita. Journal of Northwest A & F University (Natural Science Edition), 2018,46(11):115-120. (in Chinese)
[26]	靖云阁 . 白灵侧耳菌丝生理成熟期环境控制及生理指标测定[D]. 北京: 中国农业科学院, 2018.
	JING Y G . Studies of environment control and physiological test during Pleurotus tuoliensis in mycelium physiological maturity period[D]. Beijing: Chinese Academy of Agricultural Sciences, 2018. ( in Chinese)
[27]	柴红梅, 周会明, 赵静, 陈卫民, 赵永昌 . 利用自交寻找食用菌发育缺陷型基因的研究. 农业科学与技术: 英文版, 2012,13(10):2037-2043.
	CHAI H M, ZHOU H M, ZHAO J, CHEN W M, ZHAO Y C . Search development-deficient genes in edible mushroom by self-crossing. Agricultural Science＆ Technology, 2012,13(10):2037-2043. (in Chinese)
[28]	JI C, NGUYEN L N, HOU J W, HAI F I, CHEN V . Direct immobilization of laccase on titania nanoparticles from crude enzyme extracts of P. ostreatus culture for micro-pollutant degradation. Separation and Purification Technology, 2017,178:215-223.
[29]	MILLER G L . Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical chemistry, 1959,31(3):426-428.
[30]	SEBNEM K, SAADET B . Yield performances and changes in enzyme activities of Pleurotus spp. (P. ostreatus and P. sajor-caju) cultivated on different agricultural wastes. Bioresource Technology, 2010,101(9):3164-3169 .
[31]	DECKER S R, ADNEY W S, JENNINGS E, VINZANT T B, HIMMEL M E . Automated filter paper assay for determination of cellulose activity. Applied Biochemistry and Biotechnology, 2003,107(1):689-704.
[32]	LU Y H, DENG X, CHENG Z, LI Q, LIU G . Enhanced production of hybrid extracellular β-glucanase by recombinant Escherichiu coli using experimental design method. Chinese Journal of Chemical Engineering, 2007,15(2):172-177.
[33]	SHAMALA T R, SREEKANYIAH K R . Production of cellulases and D-xylanase by some selected fungal isolates. Enzyme and Microbial Technology, 1986,8(3):178-182.
[34]	SIMONIS J L, RAJA H A, SHEARER C A . Extracellular enzymes and soft rot decay: Are ascomycetes important degraders in fresh water. Fungal Diversity, 2008,31(1):135-146.
[35]	LOWRY O H, ROSENBROUGH M J, FARR A L, RANDALL R J . Protein measurement with folin-phenol reagent. The Journal of Biological Chemistry, 1951,193(1):265-275.
[36]	EGGER K N . Substrate hydrolysis patterns of post-fire ascomycetes (Pezizales). Mycologia, 1986,78(5):771-780.
[37]	POINTING S B . Qualitative methods for the determination of lignocellulolytic enzyme production by tropical fungi. Fungal Diversity, 1999,2:17-33.
[38]	SIK S, UNYAYAR A . Phanerochaete chrysosporium and Funulia trogii fort he degradation of cotton stalk and their laccase, peroxidase, ligninase and cellulose enzyme activities under semisolid state conditions. Turkish Journal of Biology, 1998,22(3):287-298.
[39]	VAN DER KOOIJ M A,, HOLLIS F, LOZANO L, ZALACHORAS I, ABAD S, ZANOLETTI O, SANDI C . Diazepam actions in the VTA enhance social dominance and mitochondrial function in the nucleus accumbens by activation of dopamine D1 receptors. Molecular psychiatry, 2018,23(3):569.
[40]	陈岗, 詹永, 杨勇, 吴振, 谢会川, 石文娟, 柴佳炎, 龚胡荣, 罗杨 . 温度对银耳胞外酶活力及营养品质特性的影响. 食品科学, 2017,38(23):113-120.
	CHEN G, ZHAN Y, YANG Y, WU Z, XIE H C, SHI W J, CHAI J Y, GONG H R, LUO Y . Effect of cultivation temperature on extracellular enzyme activities and nutritional quality characteristics of Tremella fuciformis. Food Science, 2017,38(23):113-120. (in Chinese)
[41]	LARRAYA L M, IDARETA E, ARANA D, RITTER E, PISABARRO A G, RAMIREZ L . Quantitative trait loci controlling vegetative growth rate in the edible basidiomycete Pleurotus ostreatus. Applied and Enviromental Microbiology, 2002,68(3):1109-1114.
[42]	LARRAYA L M, ALFONSO M, PISABRRO A G, RAMIREZ L . Mapping of genomic regions (Quantitative Trait Loci) controlling production and quality in industrial cultures of the edible basidiomycete Pleurotus ostreatus. Applied and Environmental Microbiology, 2003,69(6):3617-3625.
[43]	OLSON A . Genetic linkage between growth rate and the intersterility genes S and P in the basidiomycete Heterobasidion annosum s. lat. Mycological Research, 2006,110(8):979-984.
[44]	SANTOYO F GONZÁLEZ A E, TERRÓN M C, RAMÍREZ L, PISABARRO A G, . Quantitative linkage mapping of lignin-degrading enzymatic activities in Pleurotus ostreatus. Enzyme and Microbial Technology, 2008,43(2):137-143.
[45]	LARRAYA L M, PEREZ G, IRIBARREN I . Relationships between monokaryotic growth rate and mating type in the edible basidiomycete Pleurotus ostreatus. Applied and Environmental Microbiology, 2001,67(8):3385-3390.
[46]	VAN DER NEST M A, SLIPPERS B, STEENKAMP E T, DE VOS L, VAN ZYL K, STENLID J, WINGFIELD B J, WINGFIELD B D . Genetic linkage map for Amylostereum areolatum reveals an association between vegetative growth and sexual and self-recognition. Fungal Genetics and Biology, 2009,46(9):632-641.
[47]	TOKIMOTO K, KOMATSU M, TAKEMARU T . Incompatibility factors in the natural population of Lentinula edodes in Japan. Reports of the Tottori Mycological Institute, 1973,10:371-376.
[48]	MURAKAMI S, TAKEMARU T . “Puff” mutation induced by UV irradiation in Lentinus edodes (Berk.) Sing. Reports of the Tottori Mycological Institute, 1975,12:47-51.
[49]	MURAGUCHI H, ITO Y, KAMADA T, YANAGI S O . A linkage map of the basidiomycete Coprinus cinereus based on random amplified polymorphic DNAs and restriction fragment length polymorphisms. Fungal Genetics and Biology, 2003,40(2):93-102.
[50]	TERASHIMA K, MATSUMOTO T, HAYASHI E, FUKUMASA- NAKAI Y . A genetic linkage map of Lentinula edodes (shiitake) based on AFLP markers. Mycological Research, 2002,106(8):91l-917.
[51]	周会明, 柴红梅, 赵静, 魏云林, 赵永昌 . 基于SPSS的杨柳田头菇菌丝生长速度与交配型相关性分析. 西南农业学报, 2010,23(6):1992-1998.
	ZHOU H M, CHAI H M, ZHAO J, WEI Y L, ZHAO Y C . Correlation analysis between mycelial growth rate and mating type of Agrocybe salicacola based on SPSS statistics. Southwest China Journal of Agricultural Sciences, 2010,23(6):1992-1998. (in Chinese)

菌株Strain	A_xB_x (F)	A_yB_y(S)		A_xB_y (F)	A_yB_x(F)
单核体 Monokaryotic strain	001、130、173、 185	020、029、032、 071、187		026、034、036、048、 076、163、182	096、186
双核体 Dikaryotic strain	F×F: 001020、001032 F×S: 001029、001071、001187 S×S: 071130、071173、071185 F×S: 020130、020173、020185、 032130、032173、032185		F×F: 036096、036186、096034 F×S: 096026、096048、096076、 096163、096182

项目 Item	CMC	FP	βG	HC	AM	PR	LA	PPO	POD
CMC	1.000
FP	-0.226	1.000
βG	0.408	-0.149	1.000
HC	-0.007	-0.205	0.224	1.000
AM	0.590^**	-0.183	0.358	0.220	1.000
PR	0.558^*	-0.214	0.360	-0.268	0.700^**	1.000
LA	0.090	-0.225	0.316	0.043	0.453	0.609^**	1.000
PPO	0.038	0.072	0.383	0.179	0.575^*	0.531^*	0.467	1.000
POD	0.408	-0.080	-0.144	-0.272	-0.197	0.112	-0.225	-0.570^*	1.000
菌丝生长速度 Mycelial growth rate (cm?d^-1)	0.089	-0.161	0.469^*	-0.033	0.473^*	0.644^**	0.785^**	0.664^**	-0.370

自变量 Independent variable	相关系数 Related coefficient	直接通径系数 Direct path coefficient	间接通径系数 Indirect path coefficient			决策系数（R²） Decision coefficient
自变量 Independent variable	相关系数 Related coefficient	直接通径系数 Direct path coefficient	→LA（X₇）	→PO（X₈）	间接通径系数总和 Total of Indirect path coefficient	决策系数（R²） Decision coefficient
LA（X₇）	0.785	0.607		0.178	0.178	0.585
PPO（X₈）	0.664	0.380	0.284		0.284	0.360

项目 Item	CMC	FP	βG	HC	AM	PR	LA	PPO	POD
CMC	1.000
FP	0.038	1.000
βG	0.189	0.327	1.000
HC	-0.256	-0.188	0.135	1.000
AM	0.296	0.172	0.307	0.186	1.000
PR	0.173	-0.176	0.399	-0.041	0.090	1.000
LA	0.649^**	0.169	0.612^**	0.192	0.549^**	0.358	1.000
PPO	-0.470^*	-0.027	-0.617^**	-0.316	-0.475^*	-0.406	-0.864^**	1.000
POD	-0.092	0.129	0.030	0.508^*	0.241	-0.407	-0.069	-0.106	1.000
菌丝生长速度 Mycelial growth rate (cm?d^-1)	0.554^**	0.276	0.416	0.023	0.612^**	0.158	0.814^**	-0.666^**	-0.134

极性 Polarities	胞外酶平均活力 The average activity of extracellular enzymes (U)
极性 Polarities	CMC	FP	βG	HC	AM	PR	LA	PPO	POD
A_xB_x	1.85±0.38a	3.59±0.68a	1.32±0.59b	2.86±0.44a	4.56±0.56ab	1.10±0.21a	0.38±0.15b	0.27±0.03b	0.33±0.07a
A_yB_y	1.36±0.39a	4.32±0.71a	1.64±0.55ab	2.87±0.26a	3.88±0.36bc	1.11±0.14a	0.33±0.02b	0.33±0.03b	0.28±0.05a
A_xB_y	1.86±0.50a	3.89±0.85a	2.00±0.52ab	2.83±0.32a	3.49±0.38c	1.09±0.18a	0.12±0.05c	0.27±0.05b	0.28±0.03a
A_yB_x	2.04±0.50a	3.29±0.50a	2.49±0.54a	2.50±0.41a	4.87±0.12a	1.76±0.73a	0.76±0.02a	0.49±0.04a	0.35±0.05a

杨柳田头菇YAAS711菌株F₁子代胞外酶活性分析

The Extracellular Enzymes Activity of F₁ Generations and Single-spore Isolations in Agrocybe salicacola Strain YAAS711

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 51

相关文章 1

Metrics

本文评价

推荐阅读 0

交配因子 Mating factor	胞外酶平均活力 The average activity of extracellular enzyme (U)
交配因子 Mating factor	CM	FP	βG	HC	AM	PR	LA	PO	PE
A_x	1.86±0.45a	3.78±0.79a	1.76±0.55a	2.84±0.36a	3.88±0.44a	1.09±0.19a	0.22±0.08b	0.27±0.04c	0.67±0.15a
A_y	1.56±0.42a	4.03±0.65a	1.88±0.55a	2.77±0.30a	4.16±0.29a	1.30±0.31a	0.45±0.02a	0.38±0.03a	0.30±0.05b
B_x	1.92±0.42a	3.49±0.62a	1.71±0.57a	2.74±0.43a	4.66±0.41a	1.32±0.38a	0.51±0.11a	0.34±0.03ab	0.34±0.06b
B_y	1.65±0.45a	4.07±0.79a	1.85±0.54a	2.85±0.30a	3.65±0.37a	1.10±0.16a	0.21±0.03b	0.30±0.04bc	0.31±0.04b