对辣椒抗性基因Me3表现毒性的南方根结线虫 群体的SCAR分子标记

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doi:10.3864/j.issn.0578-1752.2013.05.009

摘要： 【目的】研究南方根结线虫（Meloidogyne incognita）Me3毒性变异群体的分子标记，用快速有效的分子方法来鉴定毒性变异的发生。【方法】以南方根结线虫的无毒群体以及抗性基因Me3毒性群体为材料，用根据南方根结线虫基因组设计的100对引物和19对来自文献的引物进行扩增，筛选出在3个种群中扩增的差异条带，设计SCAR引物，并建立多重PCR反应体系。【结果】得到了7对能在3个群体稳定扩增出差异条带的多态性引物，把其中的2个位点开发为SCAR标记，能够区分开3个线虫群体。多重PCR反应体系在无毒群体和Me3毒性群体中分别扩增出1条999 bp和1条629 bp的条带，在混合群体扩增出999和629 bp的2条带。【结论】成功开发了对Me3表现毒性的南方根结线虫变异群体的分子标记，可用多重PCR体系一次性鉴定Me3毒性变异的发生。

关键词: 南方根结线虫 , 分子标记 , SCAR , 毒性变异

Abstract: 【Objective】Molecular markers of virulent populations against Me3 in Meloidogyne incognita was studied in order to detect the virulence mutation rapidly and effectively. 【Method】Root-knot nematode populations including avirulent population, populations overcoming resistant gene Me3 and the mixed group of the two population were used as experiment materials, polymerase chain reaction was done with 100 primer pairs designed according to M. incognita genome and 19 pairs reported in literature to screen specific band among three populations. And subsequently SCAR primers were designed and a multiplex PCR reaction system was built. 【Result】Seven primer pairs amplifying stability bands were screened, two of which were converted into SCAR markers differentiating the three populations. Multiplex PCR from avirulent population and Me3-virulent isolates generated a fragment of 999 and 629 bp, respectively, while from the mixed group generated both of the above fragments. 【Conclusion】Virulent mutation markers were successfully developed in M. incognita, and one-step multiplex PCR can be used for identification of Me3-virulence.

Key words: Meloidogyne incognita , molecular marker , SCAR , virulence mutation

王刚, 李二峰, 茆振川, 谢丙炎, 冯东昕. 对辣椒抗性基因Me3表现毒性的南方根结线虫群体的SCAR分子标记[J]. 中国农业科学, 2013, 46(5): 943-949.

WANG Gang, LI 二Feng, MAO Zhen-Chuan, XIE Bing-Yan, FENG Dong-Xin. SCAR Molecular Markers Correlated with Populations of Meloidogyne incognita Virulent to Resistance Gene Me3[J]. Scientia Agricultura Sinica, 2013, 46(5): 943-949.

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