基于Hsp70基因的马梨形虫分类学定位分析

doi:10.3864/j.issn.0578-1752.2011.17.023

中国农业科学 ›› 2011, Vol. 44 ›› Issue (17): 3694-3700.doi: 10.3864/j.issn.0578-1752.2011.17.023

• 研究简报 • 上一篇

基于Hsp70基因的马梨形虫分类学定位分析

罗金, 刘光远, 田占成, 谢俊仁, 张萍, 沈辉, 党根生

1.中国农业科学院兰州兽医研究所/家畜疫原病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室/农业部草食动物疫病重点开放实验室
2.西安市长安区动物疾病预防控制中心

收稿日期:2011-03-14 修回日期:2011-05-23 出版日期:2011-09-01 发布日期:2011-07-10
通讯作者: 通信作者刘光远，Tel：0931-8311181；E-mail：liuguangyuan2002@sina.com
作者简介:罗金，Tel：13519644946；E-mail：luojsuxl@126.com
基金资助:
甘肃省科技重大专项计划（092NKDA031）

Taxonomic Status Assays of Theileria equi Based on Hsp70 Gene

LUO Jin, LIU Guang-Yuan, TIAN Zhan-Cheng, XIE Jun-Ren, ZHANG Ping, SHEN Hui, DANG Gen-Sheng

1.中国农业科学院兰州兽医研究所/家畜疫原病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室/农业部草食动物疫病重点开放实验室
2.西安市长安区动物疾病预防控制中心

Received:2011-03-14 Revised:2011-05-23 Online:2011-09-01 Published:2011-07-10

摘要/Abstract

摘要： 【目的】为了进一步确定马泰勒虫的分类学定位。【方法】根据GenBank上登录的马巴贝斯虫Hsp70基因序列（AB248743.1），设计引物TeHsp70F, TeHsp70R，以马泰勒虫基因组DNA为模板进行扩增，获得全长为1 920 bp的核酸片段。将该基因序列与GenBank中23种已知虫种的相应序列进行分析比较。【结果】该片段编码639个氨基酸，其疏水性氨基酸达到208个，极性氨基酸167个。同一性分析显示，该基因片段与报道的马巴贝斯虫Hsp70基因（B. equi BAF02625.1）亲缘关系最近，其次是小泰勒虫(T. parva XP764717.1)和环形泰勒虫(T. annulata AAA30130.1)，而与感染马属动物的另一个虫种驽巴贝斯虫(B. caballi BAF02619.1)亲缘关系较远。【结论】马泰勒虫Hsp70基因的克隆及系统发育分析显示，之前被定名为马巴贝斯虫（B. equi）的虫种隶属于泰勒虫虫种。本试验为马巴贝斯虫正确更名为马泰勒虫（T. equi）的分类地位提供了又一佐证。

关键词: 马泰勒虫, 努巴贝斯虫, 热休克蛋白(Hsp70), 分类学定位

Abstract: 【Objective】 The objective of the study is to determine the taxonomic status of Theileria equi (T. equi). 【Method】 According to the Hsp70 gene sequence (AB248743.1) of Babesia equi (B.equi) in GenBank, the primers TeHsp70F and TeHsp70R were designed. And a fragment with the length of 1 920 bp was obtained by PCR. The gene sequence was compared with other 23 amino acids sequences. 【Result】 The fragment encods 639 amino acids, 208 hydrophobic amino acids and 167 polar amino acid. Identity analysis showed that T. equi has a closest relationship with B.equi (BAF02625.1), and has a little relationship with T. parva (XP764717.1) and T. annulata (AAA30130.1), while T. equi has a distant relationship with B. caballi (BAF02619.1), which infects equine. 【Conclusion】 It was a correct taxonomic status for B. equi to Theileria species. And the taxonomic status was error before, for T. equi to Babesia species. In this paper, a proof was provided for taxonomic status of Theileria equi.

Key words: Theileria equi, Babesia caballi, Heat shock protein (Hsp70), taxonomic status

罗金, 刘光远, 田占成, 谢俊仁, 张萍, 沈辉, 党根生. 基于Hsp70基因的马梨形虫分类学定位分析[J]. 中国农业科学, 2011, 44(17): 3694-3700.

LUO Jin, LIU Guang-Yuan, TIAN Zhan-Cheng, XIE Jun-Ren, ZHANG Ping, SHEN Hui, DANG Gen-Sheng. Taxonomic Status Assays of Theileria equi Based on Hsp70 Gene[J]. Scientia Agricultura Sinica, 2011, 44(17): 3694-3700.

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基于Hsp70基因的马梨形虫分类学定位分析

Taxonomic Status Assays of Theileria equi Based on Hsp70 Gene

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