关键词: 奶牛')">奶牛, 子宫内膜细胞, CYP450, 复方益母生化散, 免疫功能

Abstract:

【Objective】 The aim of the study is to investigate the acting mechanism of Yimu Shenghuasan on dairy cow endometrial cells. 【Method】 In vitro experiment, the primary cultured endometrial cells in cows were isolated and the inflammatory models were made by lipopolysaccharide (LPS) induction. The inflammatory cells were treated with gradient concentrations of herbal medicine prescription Yimu Shenghuasan for 48h and 72h, then the Western blot was used to detect the expression of CYP450. In vivo experiment, the suppository Yimu Shenghuasan was put into the endometritis dairy cows to detect the content of IgG and IgA in sera. 【Results】 The expression of cytochrome P450 in dairy cow endometrial cells increased gradually by Western blot; compared with the control group, the content of IgG, IgA increased significantly; 【Conclusion】 CYP450 in the inflammatory cells expressed significantly by treatment of 2 000 µg•mL-1 Yimu Shenghuasan for 48h.

Key words: dairy cow')">dairy cow, endometrial cell, CYP450, Yimu Shenghuasan, immune function