用小RNA深度测序鉴定广西冬种马铃薯病毒

doi:10.3864/j.issn.0578-1752.2013.19.013

中国农业科学 ›› 2013, Vol. 46 ›› Issue (19): 4075-4081.doi: 10.3864/j.issn.0578-1752.2013.19.013

用小RNA深度测序鉴定广西冬种马铃薯病毒

宋静静1, 蒙姣荣12, 邹承武3, 李璞3, 王志强2, 陈保善23

1.广西大学农学院，南宁530004
2.广西大学亚热带农业生物资源保护与利用国家重点实验室，南宁530004
3.广西大学生命科学与技术学院，南宁530004

收稿日期:2013-04-03 出版日期:2013-10-01 发布日期:2013-06-04
通讯作者: 通信作者陈保善，Tel：0771-3225260；Fax：0771-3237873；E-mail：chenyaoj@gxu.edu.cn
作者简介:宋静静，Tel：15878806201；E-mail：songjing2345@163.com
基金资助:
国家重点基础研究“973”计划项目（2006CB101900）

Identification of Viruses from Potato Planted in Winter in Guangxi by Small RNA Deep Sequencing

SONG Jing-Jing-1, MENG Jiao-Rong-12, ZOU Cheng-Wu-3, LI Pu-3, WANG Zhi-Qiang-2, CHEN Bao-Shan-23

1.College of Agriculture, Guangxi University, Nanning 530004
2.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004
3.College of Life Science and Technology, Guangxi University, Nanning 530004

Received:2013-04-03 Online:2013-10-01 Published:2013-06-04

摘要/Abstract

摘要： 【目的】对广西冬种马铃薯病毒进行鉴定，为无病毒种薯选择和大田防治提供依据。【方法】2012年在马铃薯主要产区采集具有明显病毒病症状的样品，在血清学ELISA检测的基础上，进一步用小RNA深度测序对按症状分类的样品进行混合样本的病毒种类鉴定，再用RT-PCR方法对分组混合样本进行验证。【结果】在109个样本中检测到马铃薯卷叶病毒（Potato leafroll virus，PLRV）、马铃薯A病毒（Potato virus A，PVA）、马铃薯H病毒（Potato virus H，PVH）、马铃薯M病毒（Potato virus M，PVM）、马铃薯S病毒（Potato virus S，PVS）、马铃薯Y病毒（Potato virus Y，PVY）和马铃薯纺锤块茎类病毒（Potato spindle tuber viroid，PSTVd），同时发现PVS有丰富的株系分化。【结论】近年广西冬种马铃薯病毒种类增多，病症多样，亟需加强种薯管理，培育无毒健康种薯。

关键词: 小RNA高通量测序 , 广西 , 冬种马铃薯 , 病毒病害

Abstract: 【Objective】The objective of this study is to identify viruses infecting potato planted in winter in Guangxi for establishing a future healthy potato seed program and better disease control in the field. 【Method】One hundred and nine samples with viral disease-like symptoms were collected from potato plants in the fields in 2012 and subject to initial ELISA screening before they were pooled and analyzed by small RNA deep sequencing. Viruses identified by small RNA sequencing were validated by virus-specific RT-PCR from selected groups of samples.【Result】Potato leafroll virus (PLRV), Potato virus A (PVA), Potato virus H (PVH), Potato virus M (PVM), Potato virus S (PVS), Potato virus Y (PVY), and Potato spindle tuber viroid (PSTVd) were detected in the samples and PVS was found to have a rich genetic diversity.【Conclusion】Potato planted in winter in Guangxi were infected with many viruses including two viruses and a viroid that had not been reported in the region before. Thus, a virus-free healthy potato seed program is needed in urgency.

Key words: small RNA deep sequencing , Guangxi , potato planted in winter , viruses disease

宋静静1, 蒙姣荣12, 邹承武3, 李璞3, 王志强2, 陈保善23. 用小RNA深度测序鉴定广西冬种马铃薯病毒[J]. 中国农业科学, 2013, 46(19): 4075-4081.

SONG Jing-Jing-1, MENG Jiao-Rong-12, ZOU Cheng-Wu-3, LI Pu-3, WANG Zhi-Qiang-2, CHEN Bao-Shan-23. Identification of Viruses from Potato Planted in Winter in Guangxi by Small RNA Deep Sequencing[J]. Scientia Agricultura Sinica, 2013, 46(19): 4075-4081.

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用小RNA深度测序鉴定广西冬种马铃薯病毒

Identification of Viruses from Potato Planted in Winter in Guangxi by Small RNA Deep Sequencing

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