中国农业科学 ›› 2007, Vol. 40 ›› Issue (10): 2296-2302 .

• 园艺 • 上一篇    下一篇

香菇担孢子交配型比例偏分离的遗传分析

程水明,林范学   

  1. 黄冈师范学院生命科学与工程学院
  • 收稿日期:2006-04-01 修回日期:2006-12-05 出版日期:2007-10-10 发布日期:2007-10-10

Genetic analysis of distorted segregation ratio of mating types in Lentinula edodes

  1. 黄冈师范学院生命科学与工程学院
  • Received:2006-04-01 Revised:2006-12-05 Online:2007-10-10 Published:2007-10-10

摘要: 【目的】验证香菇中担孢子交配型因子分离是否存在普遍意义的偏分离现象及其在栽培菌株与野生菌株中的表现。【方法】以17个野生和栽培香菇菌株为样本,采用交配型分析、OWE-SOJ技术鉴定孢子的交配型,对各类交配型的比例进行统计分析。【结果】占供试总数64.71%的菌株担孢子交配型不呈预期的分离比,其中12个供试栽培菌株中有9个,5个供试野生菌株中有2个;偏分离菌株中均有亲本型孢子数量多于重组型孢子的趋势,偏分离双核菌丝体的F1代担孢子的核型主要取决于双核亲本的组成。【结论】香菇中担孢子交配型因子分离偏离理论预期是一种统计学意义的普遍现象;偏离程度栽培菌株大于野生菌株。

关键词: 交配型因子, 担孢子, 香菇, 偏分离

Abstract: This study made 17 strains of Lentinula edodes,including wild strains and cultivated strains which well-popularized around China as materials, statistically analyzed the ratios of spores from different aspects via mating types analysis and OWE-SOJ technique. Results from this study firstly systematically identified skewed expected distribution of mating-type factors segregation in Lentinula edodes spores has commonly statistical meanings in wild and cultivated strains. Genetic analysis to positive and negative parental-recombined fruiting showed that nuclear type of F1 progeny spores among those strains segregated theoretically distribution mainly depended on combined state of parental dikaryons, predominant spores were those with mating type identical to dikaryotic parent, indicating that the genetic basis of segregation distortion of spores is different from that of protoplast monokaryons in which B factor takes predominant responsibility for that phenomenon, it cooperate A factor with B factor to influence the ratio of spores.

Key words: Mating-type factor, basidiospore, Lentinula edodes, segregation distortion,