关键词: 柞蚕微孢子, 核糖体基因, 转录间隔区, 排列顺序, 系统分析

Abstract: 【Objective】This paper examines the sequence and organization of the ribosomal RNA gene, the ITS region of Nosema antheraeae, and present molecular biological evidence of Nosema antheraeae taxonomy. 【Method】To obtain the phlogenetic tree and sequence distance, specific primers were used for PCR amplification, then clone and nucleotide sequences were aligned using Clustal W of DNAStar software. 【Result】The outcome entailed a full small subunit rRNAs (SSU rRNA) gene, the internal transcribed spacer (ITS), 5S rRNA gene and part of the large subunit rRNAs (LSU rRNA) gene of Nosema antheraeae. 【Conclusion】The organization of the ribosomal RNA gene of Nosema antheraeae follows a special organizational pattern: LSU-ITS1-SSU-ITS2-5S. Elucidating phylogenetic relationships of microsporidia species by combining information from SSU rRNA and ITS sequence is a new experiment.

Key words: Nosema antheraeae, Ribosomal RNA gene, ITS, Organization, Phylogenetic analysis