关键词: 砀山梨, 炭疽病, 核糖体DNA转录间隔区, 抑菌药剂

Abstract:

【Objective】 To identify the pathogenic fungus of a serious disease, which had caused a severe damage to pear in Dangshan County, Anhui Province in recent years and screen out effective chemical for inhibition of its mycelium growth and conidiophores germination. 【Method】 Isolates from diseased fruits and leaves which were collected from different pear varieties in the field were cultured on PDA medium and purified by single spore culture, respectively. Identification of the pathogenic fungus was carried out according to the characteristics of fungal morphology, pathogenicity and by rDNA-ITS sequence analysis. 【Result】 Fungal morphologic characteristics of six purified pathogenic strains from different samples were the same, which were similar with the reported anthracnose pathogen Colletotrichum spp.. The symptoms induced by artificial inoculation with the fungal isolates were like the symptoms in the field, too. Further, the six strains belonged to the same pathogen which was analyzed by rDNA-ITS sequence, the rDNA-ITS sequences were the same as that of anthracnose pathogens, Colletotrichum sp. (ID FJ233185), Glomerella cingulata (ID AB219012) and C. gloeosporioides (ID EU200455), respectively. Mycelium growth and conidiophores germination could be controlled effectively by tebuconazole 430 g•L-1 SC and thiophanate-methyl 70% WP, respectively. 【Conclusion】 It is concluded that the disease on Chinese ‘Dangshansuli’ pear is caused by Colletotrichum gloeosporioides, and its teleomorph is Glomerella cingulata. There is a significant difference between treatments in inhibition of mycelium growth and conidiophores germination with different chemicals.

Key words: Dangshan pear, anthracnose, rDNA-ITS, screening fungicides