中国农业科学 ›› 2006, Vol. 39 ›› Issue (7): 1457-1463 .

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

猪体外胚胎生产的研究进展

周洪彬,肖 雄,包 磊,李跃民   

  • 收稿日期:2005-09-19 修回日期:2005-12-30 出版日期:2006-07-10 发布日期:2006-07-10

In vitro Production of Porcine Embryo

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  • Received:2005-09-19 Revised:2005-12-30 Online:2006-07-10 Published:2006-07-10

摘要: 与牛羊等动物的体外胚胎生产(in vitro production,IVP)技术相比,猪IVP技术尚不成熟。猪IVP主要存在卵母细胞的细胞质成熟差、多精子受精率高和胚胎质量差的问题,这严重制约了该繁殖生物技术在转基因猪生物反应器领域的应用。笔者在查阅近10年的国内、外相关研究后,从如何优化猪卵母细胞的体外成熟(in vitro maturation,IVM)、体外受精(in vitro fertilization,IVF)和胚胎的体外培养(in vitro culture,IVC)3个方面进行了探讨。

关键词: 猪, 体外成熟, 体外受精, 体外培养, 体外胚胎生产

Abstract: In recent years, progress has been achieved in the production of pig embryos through IVM and IVF techniques, However, there are still some problems, and factors restricting the application of the reproductive biotechnology, for example, poor cytoplasmic maturation、highly polyspermy and poor embryo development. This review will focus on porcine oocytes maturation, fertileization and embryo culture.

Key words: Porcine, In vitro maturation, In vitro fertilization, In vitro culture, In vitro production of embryo