中国农业科学 ›› 2006, Vol. 39 ›› Issue (12): 2582-2589 .

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

中缅树鼩孟德尔性状纯化方法的研究

常洪,于永梅,钱耕荪,冀德君,吴一迁   

  1. 扬州大学
  • 收稿日期:2005-08-31 修回日期:2006-06-05 出版日期:2006-12-10 发布日期:2006-12-10
  • 通讯作者: 常洪

Studies on way of purifying Mendelian characters in Tupaia belangeri

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  1. 扬州大学
  • Received:2005-08-31 Revised:2006-06-05 Online:2006-12-10 Published:2006-12-10

摘要: 【目的】寻找中缅树鼩孟德尔性状的适宜纯化方法。【方法】以Rife-Buranamanas母子性状组合频率定律鉴别了中缅树鼩8种孟德尔性状的遗传机制;以群体遗传学和概率矩阵方法探讨了动物克隆、超数排卵与胚胎移植、“理想型横交”和多元测交四种选育体制对中缅树鼩驯化群孟德尔性状纯化和保持一般的遗传多样性的效率。【结果】在中缅树鼩基因组背景下,野生型毛色、暗色眶缘、白色腹毛、有季节性红斑、肉色掌底皮肤、非竖耳、圆尾和对称乳房是显性孟德尔特征;对于规模既定的群体而言,不同体制下一代间淘汰率之比也就是近交增量、基因多样度损失量之比;就性状纯化的效应而言,多元测交体制优于“理想型横交”。以普通果蝇(Drosophila melanogaster)野生型和3个突变种为试验材料,模拟树鼩的纯化选育,取得了吻合于理论探讨的结果。【结论】本文认为多元测交体制是较好的纯化方法。

关键词: 中缅树鼩, 孟德尔特性, 纯化方法

Abstract: This treatise, on the basis of Rife-Buranamanas's law about frequencies of trait combinations between females and their children, discriminated genetic machinism of 8 kinds of Mendelian characters in Tupaia belanger, by means of population genetics and probability matrix probed into the efficiency of 4 different breeding systems (animal coloing, superovulation & embryo transfer, “breeding of habrid animals with desirable phenotype in themselves” and multiple testcross) in maintaining general genetic diversity while purifying the Mendelian characters in domesticating colony of Tupaia belangeri. It was confirmed that, in setting of genome of Tupaia belangeri, the coating color of wild type, black orbit, white fur of abdomen, seasonal spot, flesh color of palm, flat ear, round tail and symmetry breast are dominant Mendelain characteristics; as far as the breed with definite scale is concerned, the ratio between percentages of the cull of next generations under different systems is also the ratio between inbreeding increments under these systems and losses of gene heterozygosity under these systems. If n stands for the number of breeding characteristics based on dominant gene and t the number of generations selected, then probabilities of homozygosized individuals at all (as many as n) the loci in the tree shrew populations under the two sestems of “breeding of hybrid animals with desirable phenotype in themselves” and multiple testcross are in such a sequence: , In regard to purification, the latter is higher in efficiency than the former. Taking the wild type and 3 mutation types of Drosophila melanogaster as subject, simulated the selection and breeding meant to purify population of the tree shrew and the results conformed to theoretic investigation.

Key words: Tupaia belangeri, Mendelian characterestic, purifacation