中国农业科学 ›› 2006, Vol. 39 ›› Issue (11): 2237-2242 .

• 植物保护 • 上一篇    下一篇

基因表达分析稻瘟菌7个Rho GTP酶的关系

郑 武,陈继圣,郑士琴,刘文德,陈 晓,周 洁,鲁国东,王宗华   

  • 收稿日期:2006-03-05 修回日期:1900-01-01 出版日期:2006-11-10 发布日期:2006-11-10

Relationship of Rho GTPase in Magnaporthe griesea Revealed by Gene Expression Analysis

Wu Zheng,JiSheng Chen,ShiQin Zheng,WenDe Liu,Xiao Chen,Jie Zhou,GuoDong Lu,   

  • Received:2006-03-05 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10

摘要: 【目的】Rho GTP酶是Ras超家族小G蛋白最主要成员。不同生物均含多种Rho GTP酶,相互之间存在密切关系,并共同参与调控多个信号转导途径。稻瘟菌基因组中存在7个Rho GTP酶,分析它们相互关系意义甚大。【方法】通过RT-PCR分析了MgCdc42不同状态下以及MgRho3插入失活状态下其它Rho GTP酶基因的表达变化。【结果】结果表明MgCdc42可能处于MgRho1、MgRho2和MgRhoX上游,对这些蛋白起正调控作用,对MgRac1和MgRho4起着负调控作用;MgRho3对MgRho1和MgCdc42的表达起正调控,对MgRac1和MgRho4的表达则起负调控作用;MgRho3与MgCdc42间关系复杂。【结论】稻瘟菌Rho GTP酶信号途径复杂,不同Rho GTP酶间存在相互调控关系,这将有助于进一步了解稻瘟菌等丝状真菌Rho GTP酶之间的相互关系。

关键词: 稻瘟菌, RhoGTP酶, 调控机制

Abstract: The Rho GTPases are major members of the Ras superfamily proteins. It is well known that there are several Rho GTPase interacting each other and regulating many signal transduction pathways in eukaryotes. To study the relationship of 7 putative Rho-GPTases in the Magnaporthe grisea genonme, RT-PCR was used to analyze the Rho GTPase gene expression patterns in different MgCdc42 and MgRho3 mutants. It was found that MgCdc42 positively controlled the expressions of MgRho1, MgRho2 and MgRhoX; negatively regulated MgRac1 and MgRho4; however MgRho3 positively regulated the expressions of MgRho1 and MgRho2, but negatively controlled MgRho4 and MgRac1. And the relationship between MgRho3 and MgCdc42 was complicated. The result will facilitate to understand the functional relationship of different GTPases in M.grisea and other filamentous fungi.