高效液相色谱-电喷雾质谱联用法分析罗汉果皂甙

中国农业科学 ›› 2005, Vol. 38 ›› Issue (10): 2096-2101 .

高效液相色谱-电喷雾质谱联用法分析罗汉果皂甙

戚向阳,张俐勤,单夏锋,陈维军,宋云飞

华中农业大学食品科技学院

收稿日期:2004-10-19 修回日期:1900-01-01 出版日期:2005-10-10 发布日期:2005-10-10
通讯作者: 戚向阳

Study on Analysis of Mogrosides by HPLC/ESI/MS2

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华中农业大学食品科技学院

Received:2004-10-19 Revised:1900-01-01 Online:2005-10-10 Published:2005-10-10

摘要/Abstract

摘要： 【目的】建立罗汉果皂甙的HPLC/ESI/MS2定性分析方法。【方法】采用高效液相色谱-电喷雾质谱联用法对罗汉果皂甙提取物进行分离分析。【结果】根据主要色谱峰的质谱特征，初步鉴定出罗汉果皂甙提取物中主要的皂甙成分为11-氧-罗汉果皂甙V（11-oxo-mogroside-V）、罗汉果皂甙V（mogroside-V）、罗汉果皂甙IV（mogroside-IV）、罗汉果皂甙VI（mogroside-VI）和赛门甙I（siamenoside-I）。【结论】对于罗汉果皂甙的分离及定性分析，高效液相色谱-电喷雾质谱(HPLC／ESI/MS2)联用分析法是一种方便快捷有效的方法。

关键词: 罗汉果(Siratia grosvenori), 罗汉果皂甙, 高效液相色谱-电喷雾质谱联用法

Abstract: 【aim】A HPLC/ESI/MS2 method was developed for the qualitative analysis of mogrosides from fruits of Siratia grosvenori.【method】HPLC/ESI/MS2 was used to separate and analyze mogroside extracts from fruits of Siratia grosvenori. 【result】The main components of mogroside in mogroside extracts were 11-oxo-mogroside-V, mogroside-V, mogroside-IV, mogroside-VI and siamenoside-I according to mass spectrometry analysis of main chromatogram peaks. 【conclusion】HPLC／ESI/MS2 is a convenient, rapid and effective method for separation and qualitatively analysis of mogrosides from fruits of Siratia grosvenori.

Key words: Siratia grosvenori, Mogrosides, HPLC/ESI/MS2

戚向阳,张俐勤,单夏锋,陈维军,宋云飞. 高效液相色谱-电喷雾质谱联用法分析罗汉果皂甙[J]. 中国农业科学, 2005, 38(10): 2096-2101 .

,,,,. Study on Analysis of Mogrosides by HPLC/ESI/MS2[J]. Scientia Agricultura Sinica, 2005, 38(10): 2096-2101 .