转番茄铁载体基因（LeIRT2）八棱海棠对缺铁胁迫的响应

中国农业科学 ›› 2005, Vol. 38 ›› Issue (05): 1024-1028 .

转番茄铁载体基因（LeIRT2）八棱海棠对缺铁胁迫的响应

渠慎春,张君毅,陶建敏,乔玉山,章镇

南京农业大学园艺学院

收稿日期:2004-06-24 修回日期:2004-10-08 出版日期:2005-05-10 发布日期:2005-05-10
通讯作者: 章镇

Physiological Reaction on Resistance to Iron Deficiency in Transgenic Lines of Malus robusta by Introducing LeIRT2 Gene

,,,,

南京农业大学园艺学院

Received:2004-06-24 Revised:2004-10-08 Online:2005-05-10 Published:2005-05-10

摘要/Abstract

摘要： 在低铁(铁浓度为10-6mol·L-1)水培条件下，2个转番茄铁载体蛋白基因八棱海棠（Malus robusta Rehd.）株系表现出较强的抗缺铁胁迫能力。叶片叶绿素含量和净光合速率显著高于对照；叶片细胞间隙CO2浓度显著低于对照；根系的质子分泌和三价铁螯合物还原酶（FCR）活性均升高，明显强于同样条件下的非转基因植株。根系扫描电镜结果显示，转基因株系根系的吸收区和根毛的表面结构皱折增多，表面积增大。

关键词: 八棱海棠, LeIRT2, 缺铁胁迫

Abstract: Two transgenic plants of Malus robusta were hydroponically planted to test their efficiency to iron-deficiency(iron concentration 10-6mol·L-1), and the tolerance was found in these transgenic plants. Their leaf chlorophyll content and net photosynthetic rate were much higher than those of control, which showed severe chlorosis under the same condition. The rate of root H+ excretion and ferric-chelate reductase activity increased in transgenic lines and substantially higher than those of control. Scanning photos of root absorption region and root hair by electronic microscope in transgenic lines and non-transgenic plants were different under same iron treatment.

Key words: Malus robusta, LeIRT2, Tolerance to iron-deficiency

渠慎春,张君毅,陶建敏,乔玉山,章镇. 转番茄铁载体基因（LeIRT2）八棱海棠对缺铁胁迫的响应[J]. 中国农业科学, 2005, 38(05): 1024-1028 .

,,,,. Physiological Reaction on Resistance to Iron Deficiency in Transgenic Lines of Malus robusta by Introducing LeIRT2 Gene[J]. Scientia Agricultura Sinica, 2005, 38(05): 1024-1028 .

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/

https://www.chinaagrisci.com/CN/Y2005/V38/I05/1024

[1]	渠慎春,王三红,陈卫平,徐长宝,章镇. 转番茄铁载体基因（LeIRT2）八棱海棠株系的鉴定[J]. 中国农业科学, 2007, 40(9): 2015-2020 .
[2]	丛郁,孙爱君,姚泉洪,章镇. 转rolC基因八棱海棠组培苗生物学特性的研究[J]. 中国农业科学, 2006, 39(12): 2563-2569 .
[3]	安华明,樊卫国. 缺铁胁迫对川梨的生理影响[J]. 中国农业科学, 2003, 36(8): 935-940 .
[4]	张凌云,翟衡,张宪法,李佳. 苹果砧木铁高效基因型筛选[J]. 中国农业科学, 2002, 35(1): 68-71 .