声频干扰对美洲斑潜蝇求偶行为的影响

doi:10.3864/j.issn.0578-1752.2025.21.007

中国农业科学 ›› 2025, Vol. 58 ›› Issue (21): 4372-4381.doi: 10.3864/j.issn.0578-1752.2025.21.007

• 豇豆“两虫一病”绿色防控研究与实践创新 • 上一篇下一篇

声频干扰对美洲斑潜蝇求偶行为的影响

邢光涛¹^,²(), 吕宝乾³^,⁴, 吴圣勇¹^,², 吴建涛⁵, 周瀛⁶, 葛瑨⁷, 张起恺³^,⁴()

¹ 中国农业科学院植物保护研究所/植物病虫害综合治理全国重点实验室，北京 100193
² 三亚中国农业科学院国家南繁研究院，海南三亚 572024
³ 中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室，海口 571101
⁴ 中国热带农业科学院三亚研究院/海南省南繁生物安全与分子育种重点实验室，海南三亚 572025
⁵ 广州甘蔗糖业研究所海南甘蔗育种场/农业农村部广东甘蔗种质资源与利用科学观测实验站，海南三亚 572025
⁶ 浙江大学海南研究院，海南三亚 572025
⁷ 中国科学院动物研究所/农业虫害鼠害综合治理研究国家重点实验室，北京 100101

收稿日期:2025-04-24 接受日期:2025-06-13 出版日期:2025-11-01 发布日期:2025-11-06
通信作者:
张起恺，E-mail：18810521205@163.com
联系方式: 邢光涛，E-mail：guangtao_xing@163.com。
基金资助:
国家重点研发计划(2024YFD1400100); 海南省重点研发项目(ZDYF2024KJTPY018); 海南省自然科学基金(325QN410)

Effects of Acoustic Interference on the Courtship Behavior of Liriomyza sativae

XING GuangTao¹^,²(), LÜ BaoQian³^,⁴, WU ShengYong¹^,², WU JianTao⁵, ZHOU Ying⁶, GE Jin⁷, ZHANG QiKai³^,⁴()

¹ Institute of Plant Protection, Chinese Academy of Agricultural Sciences/State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193
² National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, Hainan
³ Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management of Tropical Crops, Ministry of Agriculture and Rural Affairs, Haikou 571101
⁴ Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in the Nanfan Area, Sanya 572025, Hainan
⁵ Hainan Sugarcane Breeding Station of Guangzhou Sugarcane Industry Research Institute/ Guangdong Scientific Observation Test Station of Sugarcane Germplasm Resources and Utilization, Ministry of Agriculture and Rural Affairs, Sanya 572025, Hainan
⁶ Hainan Institute, Zhejiang University, Sanya 572025, Hainan
⁷ Institute of Zoology, Chinese Academy of Sciences/State Key Laboratory of Integrated Management of Pest Insects and Rodents, Beijing 100101

Received:2025-04-24 Accepted:2025-06-13 Published:2025-11-01 Online:2025-11-06

摘要/Abstract

摘要：

【目的】探究声频刺激对美洲斑潜蝇（Liriomyza sativae）求偶行为的干扰效应及其在害虫绿色防控中的应用潜力。【方法】通过定向扬声器发射不同频率声频信号，结合激光测振技术与行为录像手段，测定豇豆叶片振动响应及美洲斑潜蝇求偶交配行为参数（潜伏期、频次、持续期、交配成功率）；利用200 Hz声频开展室内美洲斑潜蝇幼虫种群控制试验，并通过田间设置声源距离梯度与时空动态监测，分析美洲斑潜蝇种群密度变化与对豇豆品质（可溶性糖、可溶性蛋白、维生素C含量）的影响。【结果】外界声频刺激下，叶片受到不同程度的振动响应进而干扰美洲斑潜蝇求偶行为。其中不同豇豆叶片对200—300 Hz声频响应最强，振速峰值达±4 mm·s^-1；200 Hz声频显著延长雄虫求偶潜伏期（（15.92±3.99）min，对照组（5.24±1.63）min，P<0.05）和持续期（（44.50±2.68）s，对照组（20.22±1.97）s，P<0.05），增加雄虫求偶尝试频次（（13.20±2.58）次，对照组（5.40±1.21）次，P<0.05），交配成功率降至20%（对照组60%）；马尔科夫链模型分析表明，中频段（200—300 Hz）对求偶行为的干扰最为显著，该频段显著阻断“求偶→雌虫回应”关键行为路径；室内试验中，200 Hz声频使幼虫数量减少42.14%（（57.67±3.18）头，对照组（99.67±9.61）头，P<0.05）；田间试验显示，随着声频播放时间的延长，近源区（0—25 m）潜道密度显著降低，但远源区（25—70 m）控制效果较弱；豇豆品质（可溶性糖、蛋白、维生素C含量）未受声频干扰造成显著不良影响。【结论】200 Hz声频通过引发豇豆叶片最大振动响应，干扰美洲斑潜蝇求偶信号传递，从而有效抑制其交配行为与种群增长，且对豇豆品质无明显负面影响。该技术为开发环境友好型害虫防控策略提供了新视角。

关键词: 美洲斑潜蝇, 声频干扰, 振速, 基质振动, 求偶行为, 潜道密度, 绿色防控

Abstract:

【Objective】The objective of this study is to investigate the disruptive effect of acoustic stimuli on the courtship behavior of Liriomyza sativae and its application potential in green pest control. 【Method】Acoustic signals of varying frequencies were emitted by directional loudspeakers. Laser vibrometry coupled with behavioral video recording was employed to measure vibrational responses of cowpea leaves and courtship and mating parameters of L. sativae (latency, frequency, duration, mating success rate). The laboratory population control trial of L. sativae larvae was carried out by using 200 Hz acoustic frequency, and the population density change of L. sativae and effect on cowpea quality (soluble sugar, soluble protein and vitamin C content) were analyzed by setting sound source distance gradient with spatiotemporal dynamic monitoring in the field. 【Result】External acoustic stimulation induced differential leaf vibrational responses, disrupting L. sativae courtship. Cowpea leaves exhibited the strongest vibrational response within 200-300 Hz with peak vibration velocity of ±4 mm·s^-1. The 200 Hz frequency significantly prolonged male courtship latency ((15.92±3.99) min vs. control (5.24±1.63) min, P<0.05) and duration ((44.50±2.68) s vs. control (20.22±1.97) s, P<0.05), increased male courtship attempts ((13.20±2.58) times vs. control (5.40±1.21) times, P<0.05), and reduced mating success rate to 20% (control: 60%). Markov chain analysis confirmed greatest behavioral disruption in the mid-frequency band (200-300 Hz), specifically blocking the critical “male courtship→female response” transition pathway. Laboratory trials demonstrated 42.14% larval number reduction under 200 Hz acoustic treatment ((57.67±3.18) individuals vs. control (99.67±9.61) individuals, P<0.05). Field data indicated that with the increase of audio playback time, the mining density in near-source zones (0-25 m) decreased significantly, though efficacy diminished in far-source areas (25-70 m). No significant adverse effects occurred on cowpea quality parameters (soluble sugar, soluble protein and vitamin C content). 【Conclusion】The 200 Hz acoustic frequency disrupts L. sativae courtship signaling by maximizing cowpea leaf vibrations, effectively suppressing mating behavior and population growth without compromising crop quality. This technique provides an innovative perspective for developing environmentally-friendly pest control strategies.

Key words: Liriomyza sativae, acoustic interference, vibration velocity, substrate-borne vibration, courtship behavior, mining density, green pest control

邢光涛, 吕宝乾, 吴圣勇, 吴建涛, 周瀛, 葛瑨, 张起恺. 声频干扰对美洲斑潜蝇求偶行为的影响[J]. 中国农业科学, 2025, 58(21): 4372-4381.

XING GuangTao, LÜ BaoQian, WU ShengYong, WU JianTao, ZHOU Ying, GE Jin, ZHANG QiKai. Effects of Acoustic Interference on the Courtship Behavior of Liriomyza sativae[J]. Scientia Agricultura Sinica, 2025, 58(21): 4372-4381.

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声频干扰对美洲斑潜蝇求偶行为的影响

Effects of Acoustic Interference on the Courtship Behavior of Liriomyza sativae

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