蛾类性信息素生物合成研究进展

doi:10.3864/j.issn.0578-1752.2024.18.008

摘要/Abstract

摘要：

昆虫性信息素特异性强、使用方便且绿色安全，利用性信息素防治农林害虫符合我国农林绿色发展的需求，应用前景广阔。解析蛾类性信息素的生物合成路径，明确其关键催化酶，可为利用合成生物学手段生产蛾类性信息素提供重要的理论依据。按照化学结构和生物合成途径的不同，蛾类性信息素可分为Type-I、Type-II、Type-III和Type-0四大类，且以前两类为主，分别占已鉴定种类的75%和15%左右，而Type-II类环氧化烯烃性信息素中，约54%为单环氧化烯烃。上述两类性信息素合成部位不尽相同，其中Type-I类性信息素在雌蛾的性腺中合成，Type-II类性信息素中的多不饱和烯烃是在绛色细胞中合成的，而环氧化反应是在性腺中进行的。在合成路径和关键催化酶方面，Type-I类性信息素是由脂肪酸代谢途径从头合成的，目前报道较多的合成相关的催化酶为去饱和酶、脂肪酰基辅酶A还原酶、醇氧化酶和乙酰基转移酶；Type-II类性信息素以食物源的亚油酸或亚麻酸为起点合成，已鉴定的关键催化酶有末端脱氢酶、载脂转运蛋白和P450环氧化酶。此外，文中还阐述了利用植物和酵母细胞生成Type-I类蛾类性信息素研究现状，探讨了目前利用合成生物学技术合成蛾类性信息素方面尚待解决的问题，提出了未来研究仍需关注的重点，为深入开发蛾类性信息素生物合成工厂提供参考。

关键词: 蛾类性信息素, 生物合成路径, 合成位点, 催化酶, 合成生物学

Abstract:

Sex pheromones are species-specific, easy-to-use, and environmental-friendly, using sex pheromones to monitor and control agricultural and forestry pests has promising prospects to meet the demands for sustainable agriculture in China. Elucidations of the moth sex pheromone biosynthetic pathways and key catalytic enzymes are of vital importance because they can serve as a theoretical basis for producing moth sex pheromones with synthetic biology strategies. According to the chemical structures and the biosynthetic pathways, moth sex pheromones are divided into four categories, namely Type-I, Type-II, Type-III, and Type-0, and the majority belongs to the former two categories, accounting for approximately 75% and 15%, respectively, of the known moth sex pheromones. Among the Type-II epoxidized sex pheromones, about 54% is monoepoxide. In terms of the biosynthetic site, Type-I sex pheromones are synthesized in the female sex pheromone glands, while Type-II hydrocarbon sex pheromones are produced in the oenocytes and only the final epoxidation occurs in the sex pheromone glands. In regards to the biosynthetic pathways and catalytic enzymes, Type-I sex pheromones are de novo synthesized from the fatty acid metabolic pathway and the involved enzymes that have been reported are fatty acyl desaturase, fatty acyl-CoA reductase, alcohol oxidase and acetyltransferase, and Type-II polyunsaturated hydrocarbons are derived from dietary linoleic and linolenic fatty acids, the identified involved enzymes include terminal desaturase, lipid transport protein lipophorin and P450 epoxidase. In addition, the current research status of the biological production of moth sex pheromones in plant and yeast cell factories is summarized. To further develop bio-factories for moth sex pheromone production with synthetic biology strategies, the remaining problems and research prospects faced in moth sex pheromone biosynthesis and bio-production are proposed as well.

Key words: moth sex pheromone, biosynthetic pathway, biosynthetic site, catalytic enzyme, synthetic biology

付楠霞, 刘栩菲, 罗宗秀, 蔡晓明, 李兆群, 边磊, 修春丽, 周利, 陈宗懋. 蛾类性信息素生物合成研究进展[J]. 中国农业科学, 2024, 57(18): 3612-3625.

FU NanXia, LIU XuFei, LUO ZongXiu, CAI XiaoMing, LI ZhaoQun, BIAN Lei, XIU ChunLi, ZHOU Li, CHEN ZongMao. Research Progress in Moth Sex Pheromone Biosynthesis[J]. Scientia Agricultura Sinica, 2024, 57(18): 3612-3625.

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链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2024.18.008

https://www.chinaagrisci.com/CN/Y2024/V57/I18/3612

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总科 <BOLD>S</BOLD>uperfamily	科 <BOLD>F</BOLD>amily	环氧化位点<BOLD>P</BOLD>osition of epoxy rings
		单环氧化<BOLD>M</BOLD>ono-epo						双环氧化Di-epo		位点未知 Unknown
				3位 3epo	6位 6epo	9位 9epo	4位^† 4epo	7位^† 7epo	11位^† 11epo	位点未知 Unknown	3和6位 3, 6-diepo	6和9 6, 9-diepo
尺蛾总科Geometroidea	尺蛾科Geometridae	31	32	24	1					6
夜蛾总科Noctuoidea	夜蛾科Noctuidae			2
夜蛾总科Noctuoidea	目夜蛾科Erebidae	3	17	52		17	1	2	1	4
螟蛾总科Pyraloidea	草螟科Crambidae					1
总数Total		34	49	78	1	18	1	2	1	10