牦牛Linc24063的克隆鉴定及其与miRNAs 表达水平的相关性分析

doi:10.3864/j.issn.0578-1752.2019.14.012

中国农业科学 ›› 2019, Vol. 52 ›› Issue (14): 2538-2547.doi: 10.3864/j.issn.0578-1752.2019.14.012

• 畜牧·兽医·资源昆虫 • 上一篇下一篇

牦牛Linc24063的克隆鉴定及其与miRNAs 表达水平的相关性分析

王会^1,²,柴志欣^1,²,朱江江^1,²,钟金城^1,²(),张成福³,信金伟³

¹西南民族大学,青藏高原动物遗传资源保护与利用教育部重点实验室,成都 610041
²西南民族大学,青藏高原动物遗传资源保护与利用四川省重点实验室,成都 610041
³西藏自治区农牧科学院, 省部共建青稞和牦牛种质资源与遗传改良国家重点实验室,拉萨 850002

收稿日期:2018-11-05 接受日期:2019-05-09 出版日期:2019-07-16 发布日期:2019-07-26
通讯作者: 钟金城
作者简介:王会,E-mail: wanghui892321@sina.cn。
基金资助:
四川省科技计划(2019YJ0257);省部共建青稞和牦牛种质资源与遗传改良国家重点实验室开放基金课题(XZNKY-2019-C-007K08);国家肉牛牦牛产业技术体系(CARS-37)

Cloning and Identification of Long-Chain Non-Coding RNA Linc24063 and Its Correlation with the Expression Level of miRNAs in Yak

WANG Hui^1,²,CHAI ZhiXin^1,²,ZHU JiangJiang^1,²,ZHONG JinCheng^1,²(),ZHANG ChengFu³,Xin JinWei³

¹Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization (Southwest Minzu University), Ministry of Education, Chengdu, 610041
²Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization Key Laboratory of Sichuan Province, Southwest Minzu University, Chengdu, 610041
³State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement, Tibet Academy of Agricultural and Animal Husbandry Sciences(TAAAS), Lhasa 850002;

Received:2018-11-05 Accepted:2019-05-09 Online:2019-07-16 Published:2019-07-26
Contact: JinCheng ZHONG

摘要/Abstract

摘要：

【目的】通过对牦牛长链非编码RNA Linc24063进行克隆鉴定,分析其在乳腺组织中与miRNAs表达量的相关性,为Linc24063通过调控miRNA发挥功能的调控机制研究提供试验依据。【方法】选取4.5岁处于第一泌乳期的健康母牦牛12头,清晨空腹屠宰后,迅速采集大脑、乳腺、肾脏、心脏、肝脏和卵巢组织,提取组织总RNA,利用5′ RACE和3′ RACE方法克隆牦牛Linc24063序列;利用生物信息学对其序列保守性、染色体定位及编码潜能进行分析,然后利用原核表达试验对其编码能力进行验证;利用RT-qPCR检测其在大脑、乳腺、肾脏、心脏、肝脏和卵巢的表达水平。利用普通牛和绵羊的miRNA数据库,结合miRanda和mireap软件预测与Linc24063具有相互作用的物种间保守miRNAs,与前人研究表明在牛不同泌乳时期乳腺组织中具有差异表达的miRNA取交集,然后对其靶基因进行GO富集和KEGG信号通路分析;最后使用皮尔逊相关系数在乳腺组织中进行Linc24063与miRNAs表达量的相关性分析。【结果】Linc24063 5′RACE和3′RACE片段大小分别为476 bp和356 bp,测序分析表明Linc24063大小为758 bp,位于牦牛21号染色体的Dlk1-Dio3印记域,与普通牛的序列保守性最高。生物信息学预测其编码潜能较低,原核表达试验进一步验证Linc24063不能有效的翻译蛋白,表明Linc24063是一个真正的长链非编码lncRNA。组织表达谱分析表明,Linc24063在牦牛乳腺组织中表达量最高,在肝脏和卵巢中表达量较低。生物信息学分析后共筛选到与Linc24063具有相互作用的物种间保守miRNAs 21个,其中有研究报道在乳腺中有差异表达的13个;13个miRNAs靶基因富集到TGF-β、PI3K-Akt、胰岛素等信号通路中,表明Linc24063可能通过这些信号通路参与牦牛乳脂和乳蛋白的生物合成过程。在乳腺组织中,Linc24063表达水平与miR-200a（P=0.001）、miR-141（P=0.02）显著负相关,与miR-27a（P=0.023）显著正相关,与miR-24（P=0.601）不具备相关性。【结论】Linc24063位于Dlk1-Dio3印记域内,在乳腺组织中具有较高表达量,且可能通过与miR-200a、miR-141和miR-27a相互作用从而参与牦牛乳脂和乳蛋白的生物合成过程。为深入探讨牦牛Linc24063在乳脂和乳蛋白合成中的生物学功能提供了基础数据。

关键词: 牦牛, Linc24063, RACE, 原核表达, 乳脂, 乳蛋白

Abstract:

【Objective】The aim of this study was to clone and to identify the protein coding potential of the long-chain non-coding RNA Linc24063 in yak, and then to analyze its correlation with the expression of miRNAs in mammary gland, which might provide basis for studying the function of Linc24063 in yak. 【Method】Twelve healthy female yaks at 4.5-year old in the first lactation period were selected as experiment animal. After fasting slaughter, the tissue samples of cerebrum, mammary gland, kidney, heart, liver and ovary were collected for total RNA extraction. Firstly, the sequence of Linc24063 was cloned by 5' RACE and 3' RACE, and the sequence conservation, chromosomal location and coding potential were analyzed by bioinformatics, then the prokaryotic expression assays were used to analyze its protein coding ability. Its expression levels in cerebrum, mammary gland, kidney, heart, liver and ovary tissue were determined by using RT-qPCR. Secondly, the conserved miRNAs interacting with Linc24063 were predicted by using the miRNA database of cattle and sheep, combined with miRanda and mireap software. The same miRNAs were obtained from the published differentially expressed miRNAs during different stages of lactation in cattle and the conserved miRNAs, and then GO enrichment and KEGG pathway analysis were performed on their target genes. Finally, Pearson correlation coefficient was used for analyzing the correlation between the expression of Linc24063 and miRNAs in the mammary gland of yak. 【Result】The length of Linc24063 5'RACE and 3'RACE fragments were 476 bp and 356 bp, respectively. Sequencing analysis showed that Linc24063 was 758 bp in length and located in the Dlk1-Dio3 imprinting domain of 21 chromosomes in yak. Bioinformatics predicts results showed that its coding potential was lower, and prokaryotic expression experiments further demonstrated that Linc24063 had no protein coding ability, suggesting that Linc24063 was a real lncRNA. Tissue expression profiling showed that Linc24063 had highest expression in mammary gland and lowest expression in liver and ovary. After bioinformatics analysis, Twenty-one conserved miRNAs interacting with Linc24063 were screened, among which 13 miRNA were differentially expressed in the mammary gland reported previously. The 13 miRNAs targets were enriched in TGF-β, PI3K-Akt, insulin and other signaling pathways, suggesting that Linc24063 might participate in the biosynthesis of milk fat and protein via these signaling pathways. In mammary gland tissue, the expression of Linc24063 was significantly negatively correlated with miR-200a (P=0.001) and miR-141 (P=0.02), and significantly positively correlated with miR-27a (P=0.023), but no correlation with miR-24 (P=0.601). 【Conclusion】The results showed that Linc24063 was located in the Dlk1-Dio3 imprinting domain and expressed higher in mammary gland, and might play an important roles in the biosynthesis of milk fat and protein via miR-200a, miR-141 and miR-27a, which would be beneficial for revealing the regulatory mechanism of Linc24063 in milk fat and protein synthesis.

Key words: Bos grunniens, Linc24063, RACE, prokaryotic expression, milk fat, milk protein

王会,柴志欣,朱江江,钟金城,张成福,信金伟. 牦牛Linc24063的克隆鉴定及其与miRNAs 表达水平的相关性分析[J]. 中国农业科学, 2019, 52(14): 2538-2547.

WANG Hui,CHAI ZhiXin,ZHU JiangJiang,ZHONG JinCheng,ZHANG ChengFu,Xin JinWei. Cloning and Identification of Long-Chain Non-Coding RNA Linc24063 and Its Correlation with the Expression Level of miRNAs in Yak[J]. Scientia Agricultura Sinica, 2019, 52(14): 2538-2547.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2019.14.012

https://www.chinaagrisci.com/CN/Y2019/V52/I14/2538

图/表 9

表1

图1

表2

图2

图3

表3

表4

表5

图4

参考文献 31

[1]	SALMENA L, POLISENO L, TAY Y, KATS L, PANDOLFI P . A ceRNA hypothesis: the Rosetta stone of a hidden RNA language? Cell, 2011,146(3):353-358. doi: 10.1016/j.cell.2011.07.014
[2]	WOOD A J, OAKEY R J . Genomic Imprinting in Mammals: Emerging Themes and Established Theories. PloS Genetics, 2006,2(11):e147.
[3]	SLEUTELS F, DENISE P B . The origins of genomic imprinting in mammals. Advances in genetics, 2002,46:119-164. doi: 10.1016/S0065-2660(02)46006-3
[4]	SAITO T, HARA S, KATO T, TAMANO M, MURAMATSU A, ASAHARA H, TAKADA S . A tandem repeat array in IG-DMR is essential for imprinting of paternal allele at the Dlk1-Dio3 domain during embryonic development. Human Molecular Genetics, 2018,27(18):3283-3292. doi: 10.1093/hmg/ddy235
[5]	ENTERINA J R, KSS E, ANDERSON C, MARSHALL E A, NG K W, LAM W L . DLK1-DIO3 imprinted locus deregulation in development, respiratory disease, and cancer. Expert Review of Respiratory Medicine, 2017,11(9):749-761. doi: 10.1080/17476348.2017.1355241
[6]	BENETATOS L, VARTHOLOMATOS G, HATZIMICHAEL E . DLK1-DIO3 imprinted cluster in induced pluripotency: landscape in the mist. Cellular and Molecular Life Science, 2014,71(22):4421-4430. doi: 10.1007/s00018-014-1698-9
[7]	WÜST S, DRÖSE S, HEIDLER J, WITTIG I, KLOCKNER I, FRANKO A, BONKE E, GÜNTHER S, GÄRTNER U, BOETTGER T, BRAUN T . Metabolic Maturation during Muscle Stem Cell Differentiation Is Achieved by miR-1/133a-Mediated Inhibition of the Dlk1-Dio3 Mega Gene Cluster. Cell Metabolism, 2018,27(5):1026-1039. doi: 10.1016/j.cmet.2018.02.022
[8]	CHARLIER C, SEGERS K, WAGENAAR D, KARIM L, BERGHMANS S, JAILLON O, SHAY T, WEISSENBACH J, COCKETT N, GYAPAY G, GEORGES M . Human-Ovine Comparative Sequencing of a 250-kb Imprinted Domain Encompassing the Callipyge (clpg) Locus and Identification of Six Imprinted Transcripts: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8. Genome Research, 2001,11(5):850-862. doi: 10.1101/gr.172701
[9]	LIU L, LUO G Z, YANG W, ZHAO X Y, ZHENG Q Y, LV Z, LI W, WU H J, WANG L, WANG X J, ZHOU Q . Activation of the Imprinted Dlk1-Dio3 region correlates with pluripotency levels of mouse stem cells. Journal of Biological Chemistry, 2010,285(25):19483-19890. doi: 10.1074/jbc.M110.131995
[10]	STADTFELD M, APOSTOLOU E, FERRARI F, CHOI J, WALSH R M, CHEN T, OOI S, KIM S Y, BESTOR T H, SHIODA T, PARK P J, HOCHEDLINGER K . Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells. Nature Genetics, 2012,44(4):398-405. doi: 10.1038/ng.1110
[11]	ZHONG Z, YE Y, GUO W, HE Y, HU W . Relationship between DLK1 gene promoter region DNA methylation and non-small cell lung cancer biological behavior. Oncology Letters, 2017,13(6):4123-4126. doi: 10.3892/ol.2017.6019
[12]	吴昊, 张运海, 凌英会 . 非编码RNA在胚胎发育过程中的作用. 畜牧兽医学报, 2017,48(1):1-4. doi: 10.11843/j.issn.0366-6964.2017.01.001
	WU H, ZHANG Y H, LING H Y . The progress of recent advances in the ncrna-mediated regulation during embryogenesis. Acta Veterinaria Et Zootechnica Sinica, 2017,48(1):1-4. (in Chinese) doi: 10.11843/j.issn.0366-6964.2017.01.001
[13]	李燕, 陈明明, 张俊星, 张林林, 李新, 郭宏, 丁向彬, 刘新峰 . 牛LncRNA-133a对骨骼肌卫星细胞增殖分化的影响. 中国农业科学, 2019,52(1):143-153.
	LI Y, CHEN M M, ZHANG J X, ZHANG L L, LI X, GUO H, DING X B, LIU X F . Effects of bovine LncRNA-133a on the proliferation and differentiation of skeletal muscle satellite cells. Scientia Agricultura Sinica, 2019,52(1):143-153. (in Chinese)
[14]	陈瑞, 于帅, 陈晓旭, 杜健, 朱振东, 潘传英, 曾文先 . 非编码RNA对哺乳动物精子发生过程的调控. 中国农业科学, 2017,50(2):380-390.
	CHEN R, YU S, CHEN X X, DU J, ZHU Z D, PAN C Y, ZENG W X . Regulatory role of noncoding RNAs during spermatogenesis. Scientia Agricultura Sinica, 2017,50(2):380-390. (in Chinese)
[15]	ZHU X, WU Y B, ZHOU J, KANG D M . Upregulation of lncRNA MEG3 promotes hepatic insulin resistance via increasing FoxO1 expression. Biochemical and Biophysical Research Communications, 2016,469(2):319-325. doi: 10.1016/j.bbrc.2015.11.048
[16]	ZHANG F W, ZENG T B, HAN Z B, HE H J, CHEN Y, GU N, JIANG H J, WU Q . Imprinting and expression analysis of a non-coding RNA gene in the mouse Dlk1-Dio3 domain. Journal of Molecular Histology, 2011,42(4):333-339. doi: 10.1007/s10735-011-9337-3
[17]	HAN Z, LIU Q, HUANG Z, CUI W, TIAN Y, YAN W, WU Q . Expression and imprinting analysis of AK044800, a transcript from the Dlk1-Dio3 imprinted gene cluster during mouse embryogenesis. Molecules and Cells, 2013,35(4):285-290. doi: 10.1007/s10059-013-2275-z
[18]	杨文志, 张明月, 王冠楠, 赵宇鹏, 张巍巍, 李世杰 . 2个印记的基因间lncRNAs位于牛Dlk1-Dio3印记区域. 畜牧兽医学报, 2016,47(9):1848-1852. doi: 10.11843/j.issn.0366-6964.2016.09.013
	YANG W Z, ZHANG M Y, WANG G N, ZHAO Y P, ZHANG W W, LI S J . Two imprinted long non-coding RNAs located in cattle dlk1-dio3 domain. Acta Veterinaria Et Zootechnica Sinica, 2016,47(9):1848-1852. (in Chinese) doi: 10.11843/j.issn.0366-6964.2016.09.013
[19]	ZHANG M, ZHAO Y, WANG G, LI D, CHEN W, ZHANG C, L S. An imprinted long noncoding RNA located between genes Meg8 and Meg9 in the cattle Dlk1-Dio3 domain. Genetica, 2017,145(1):1-7. doi: 10.1007/s10709-016-9939-5
[20]	LI M, SUN X, CAI H, SUN Y, PLATH M, LI C, LAN X, LEI C, LIN F, BAI Y, CHEN H . Long non-coding RNA ADNCR suppresses adipogenic differentiation by targeting miR-204. Biochimcia et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 2016,1859(7):871-882. doi: 10.1016/j.bbagrm.2016.05.003
[21]	WANG H, SHI H, LUO J, YI Y, YAO D, ZHANG X, MA G, LOOR J J . Mir-145 regulates lipogenesis in goat mammary cells via targeting insig1 and epigenetic regulation of lipid-related genes. Journal of Cellular Physiology, 2017,232(5):1030-1040. doi: 10.1002/jcp.v232.5
[22]	CHEN C, RIDZON D A, BROOMER A J, ZHOU Z, LEE D, NGUYEN J, BARBISIN M, XU N, MAHUVAKAR V, ANDERSEN M, LAO K, LIVAK K, GUEGLER K . Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Research, 2005,33(20):e179. doi: 10.1093/nar/gni178
[23]	LI Z, LIU H, JIN X, LO L, LIU J . Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation. BMC Genomics, 2012,13(1):731. doi: 10.1186/1471-2164-13-731
[24]	CAI X, LIU Q, ZHANG X, REN Y, LEI X, LI S, CHEN Q, DENG K, WANG P, ZHANG H, SHI D . Identification and analysis of the expression of microRNA from lactating and nonlactating mammary glands of the Chinese swamp buffalo. Journal of Dairy Science, 2017,100(3):1971-1986. doi: 10.3168/jds.2016-11461
[25]	ROCHA S T D, EDWARDS C A, ITO M, OGATA T, FERGUSON- SMITH A C . Genomic imprinting at the mammalian Dlk1-Dio3 domain. Trends in Genetics, 2008,24(6):306-316. doi: 10.1016/j.tig.2008.03.011
[26]	WAKEFIELD L M, PIEK E, BÖTTINGER E P . TGF-β Signaling in Mammary Gland Development and Tumorigenesis. Journal of Mammary Gland Biology and Neoplasia, 2001,6(1):67-82. doi: 10.1023/A:1009568532177
[27]	RÄDLER P D, WEHDE B L, WAGNER K U . Crosstalk between STAT5 activation and PI3K/AKT functions in normal and transformed mammary epithelial cells. Molecular and Cellular Endocrinology, 2017,451:31-39. doi: 10.1016/j.mce.2017.04.025
[28]	ZHANG T, HUANG J, YI Y, ZHANG X, JUAN L L, CAO Y, SHI H, LUO J . Akt Serine/Threonine Kinase 1 Regulates De Novo Fatty Acid Synthesis through mTOR/SREBP1 Axis in Dairy Goat Mammary Epithelial Cells. Journal of Agricultural and Food Chemistry, 2018,66(5):1197-1205. doi: 10.1021/acs.jafc.7b05305
[29]	NAGAOKA K, ZHANG H, WATANABE G, TAYA K . Epithelial Cell Differentiation Regulated by MicroRNA-200a in Mammary Glands. Plos One, 2013,8(6):e65127. doi: 10.1371/journal.pone.0065127
[30]	林先滋, 罗军, 张犁苹, 朱江江, 石恒波, 苟德明 . miR-200a对奶山羊乳腺上皮细胞乳脂合成相关基因mRNA表达的影响. 畜牧兽医学报, 2012,43(7):1028-1036.
	LIN X Z, LUO J, ZHANG L P, ZHU J J, SHI H B, GOU D M . The Effect of miR-200a on Gene mRNA Expression Related to Milk Fat Synthesis in Dairy Goat Mammary Gland Epithelial Cells. Chinese Journal of Animal & Veterinary Sciences, 2012,43(7):1028-1036.(in Chinese)
[31]	LIN X Z, LUO J, ZHANG L P, WANG W, SHI H B, ZHU J J . MiR-27a suppresses triglyceride accumulation and affects gene mRNA expression associated with fat metabolism in dairy goat mammary gland epithelial cells. Gene, 2013,521(1):15-23. doi: 10.1016/j.gene.2013.03.050

基因名称 Gene name	引物序列（5′-3′） Primer sequence (5′-3′)	产物大小 Product length (bp)
Linc24063	F:ACTGTGAACATAGATGGTGAGG	141
	R:CACGGGGTCACAAAGAGTCA
RPS9	F:GTGGTGAACATCCCGTCCTT	130
	R:GCCACTGCACCTTGTAACAC
UXT	F:CGAGGCTTTCATCTCTGACG	138
	R:TCCGAGTGATTAGCTTCCTGG
18S rRNA	F:GTGGTGTTGAGGAAAGCAGACA	79
	R:CGATCCCTGTCCTCACCTCATC
MiR-200a	RT:GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACaacatcgt
	F:CTGGtaacactgtctggta	70
MiR-24	RT:GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACctgttcct
	F:GGAtggctcagttcagc	69
MiR-27a	RT:GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACcggaactt
	F:GGCacttcacagtggct	69
MiR-141	RT:GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACccatcttt
	F:GGCataacactgtctggt	69
miRNA universal primer	GTGCAGGGTCCGAGGT

基因 Gene	编码/非编码 Coding/Noncoding	编码潜能 Coding probability	Fickett 分 Fickett score	等电点 Isoelectric point
Linc24063	Noncoding	0.01	0.35	9.52
Meg9	Noncoding	0.17	0.36	7.90
CSN2	coding	1.00	0.45	5.89

Name	成熟体序列（5′-3′）Mature sequence(5′-3′)
bta-miR-137	UUAUUGCUUAAGAAUACGCGUAG
bta-miR-139	UCUACAGUGCACGUGUCUCCAGU
bta-miR-141	UAACACUGUCUGGUAAAGAUGG
bta-miR-154a	UAGGUUAUCCGUGUAGCCUUCG
bta-miR-200a	UAACACUGUCUGGUAACGAUGUU
bta-miR-221	AGCUACAUUGUCUGCUGGGUUU
bta-miR-223	UGUCAGUUUGUCAAAUACCCCA
bta-miR-24	UGGCUCAGUUCAGCAGGAACAG
bta-miR-27a	AGGGCUUAGCUGCUUGUGAGCA
bta-miR-30b-5p	UGUAAACAUCCUACACUCAGCU
bta-miR-369-3p	AAUAAUACAUGGUUGAUCUUU
bta-miR-374a	UUAUAAUACAACCUGAUAAGUG
bta-miR-376b	AUCAUAGAGGAAAAUCCAUGUU
bta-miR-378b	ACUGGACUUGGAGUCAGAAGGC
bta-miR-378c	ACUGGACUUGGAGUCAGAAGU
bta-miR-455-3p	GCAGUCCAUGGGCAUAUACACU
bta-miR-487a	AAUCAUACAGGGACAUCCAGU
bta-miR-500	UAAUCCUUGCUACCUGGGUGAGA
bta-miR-543	AAACAUUCGCGGUGCACUUCUU
bta-miR-551a	GCGACCCAAUCUUGGUUUCCA
bta-miR-655	AUAAUACAUGGUUAACCUCUCU

GO条目 Go Term	基因数目 Gene count	富集倍数 Fold enrichment	P值 P value
Nucleus细胞核	201	1.63	7.98E-14
Cytoplasm细胞质	171	1.28	2.06E-04
Regulation of transcription from RNA polymerase II promoter RNA聚合酶II启动子转录的调控	116	1.98	4.76E-06
Nucleoplasm 核质	83	1.48	2.68E-04
Metal ion binding金属离子结合	71	1.42	0.00231
Cytosol胞液	60	1.37	0.011998
RNA polymerase II core promoter proximal region sequence-specific DNA binding DNA特异性结合RNA聚合酶II核心启动子近区序列	52	2.92	3.51E-08
Transcription, DNA-templated DNA为模板的转录	42	1.57	0.004197
Transcriptional activator activity, RNA polymerase II core promoter proximal region RNA聚合酶II核心启动子近区转录激活活性	33	3.05	2.15E-06
Sequence-specific binding序列特异性结合
Chromatin binding染色质结合	30	2.22	8.71E-05

信号通路条目 Pathway term	基因数 Input number	P值 P-value	校正后P值 Corrected P-value
TGF-beta signaling pathway TGF-β信号通路	16	1.46E-06	8.22E-05
PI3K-Akt signaling pathway PI3K-Akt信号通路	28	0.000267	0.0063
Axon guidance 轴突导向	18	0.000392	0.00865
ErbB signaling pathway ErbB 信号通路	10	0.002087	0.03393
Chronic myeloid leukemia慢性粒细胞白血病	9	0.002965	0.04313
FoxO signaling pathway FoXO信号通路	13	0.003366	0.04738
Pathways in cancer 癌症通路	27	0.003372	0.04783
Insulin signaling pathway胰岛素信号	13	0.003495	0.04786
Maturity onset diabetes of the young糖尿病	5	0.003572	0.04789
MAPK signaling pathway MAPK信号通路	19	0.003611	0.04952

牦牛Linc24063的克隆鉴定及其与miRNAs 表达水平的相关性分析

Cloning and Identification of Long-Chain Non-Coding RNA Linc24063 and Its Correlation with the Expression Level of miRNAs in Yak

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 31

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	阿依木古丽·阿不都热依木,阿尔祖古丽·阿依丁,王家敏,石嘉琛,马芳芳,蔡勇,乔自林. 大豆异黄酮对牦牛卵巢颗粒细胞增殖和凋亡的影响[J]. 中国农业科学, 2022, 55(8): 1667-1675.
[2]	冉宏标,赵丽玲,王会,柴志欣,王吉坤,王嘉博,武志娟,钟金城. LncFAM200B对牦牛肌内前体脂肪细胞脂质沉积的影响[J]. 中国农业科学, 2022, 55(13): 2654-2666.
[3]	徐翔,解屹,宋丽云,申莉莉,李莹,王勇,刘明宏,刘东阳,王小彦,赵存孝,王凤龙,杨金广. 高效靶向降解烟草花叶病毒核酸的dsRNA筛选与大量制备[J]. 中国农业科学, 2021, 54(6): 1143-1153.
[4]	刘孝贺,仇贵生,佟兆国,张怀江,闫文涛,岳强,孙丽娜. 桃小食心虫化学感受蛋白CSP16配体结合特性[J]. 中国农业科学, 2021, 54(5): 945-958.
[5]	秦健辉,李金桥,赵旭,李克斌,曹雅忠,尹姣. 铜绿丽金龟气味结合蛋白AcorOBP11的表达纯化及功能分析[J]. 中国农业科学, 2021, 54(14): 3017-3028.
[6]	解昆仑,刘莉铭,刘美,彭斌,吴会杰,古勤生. 小西葫芦黄花叶病毒dsRNA的原核表达及其对ZYMV的防治效果[J]. 中国农业科学, 2020, 53(8): 1583-1593.
[7]	李雪茹,师希雄,王建忠,张攀高,田铸,韩玲. 一氧化氮合成酶抑制剂对宰后成熟过程中牦牛肉品质的影响[J]. 中国农业科学, 2020, 53(8): 1617-1626.
[8]	苗建军,彭忠利,高彦华,柏雪,谢昕廷. 日粮中添加小肽对育肥牦牛生产性能和消化道PepT1 mRNA表达的影响[J]. 中国农业科学, 2020, 53(23): 4950-4960.
[9]	田媛,王力,龙凤,昝林森,成功. 人溶菌酶密码子优化及其在牛乳腺细胞中高效表达[J]. 中国农业科学, 2020, 53(18): 3805-3817.
[10]	潘阳阳,王萌,芮弦,王立斌,何翃闳,王靖雷,马睿,徐庚全,崔燕,樊江峰,余四九. IGF-1调控RBM3表达抑制低温应激诱导牦牛卵丘细胞凋亡[J]. 中国农业科学, 2020, 53(11): 2285-2296.
[11]	黄向月,熊显荣,韩杰,杨显英,王艳,王斌,李键. KDM1A在牦牛卵泡发育过程中的表达[J]. 中国农业科学, 2019, 52(24): 4624-4631.
[12]	毕可然,李银,韩凯凯,赵冬敏,刘青涛,刘宇卓,黄欣梅,杨婧. 鸭寡腺苷酸合成酶样蛋白原核表达及多克隆抗体制备[J]. 中国农业科学, 2019, 52(23): 4429-4436.
[13]	李玲,谭瑶,周晓榕,庞保平. 沙葱萤叶甲气味结合蛋白GdauOBP20的基因克隆、原核表达及其结合特性[J]. 中国农业科学, 2019, 52(20): 3705-3712.
[14]	李都, 牛长缨, 李峰奇, 罗晨. 斑翅果蝇气味结合蛋白OBP56h与小分子化合物的结合特征[J]. 中国农业科学, 2019, 52(15): 2616-2623.
[15]	谢洁,王明,丁红映,李青,王万兴,熊兴耀,秦玉芝. 马铃薯低温响应的ScmiR390-5p及其靶基因表达与结构分析[J]. 中国农业科学, 2019, 52(13): 2295-2308.