一种分析土壤中酚酸类物质含量的新方法—以连作苹果园土壤为试材

doi:10.3864/j.issn.0578-1752.2013.21.025

中国农业科学 ›› 2013, Vol. 46 ›› Issue (21): 4612-4619.doi: 10.3864/j.issn.0578-1752.2013.21.025

一种分析土壤中酚酸类物质含量的新方法—以连作苹果园土壤为试材

尹承苗1, 王功帅1, 李园园2, 车金水3, 沈向1, 陈学森1, 毛志泉1, 吴树敬1

1.山东农业大学园艺科学与工程学院/作物生物学国家重点实验室，山东泰安271018
2.青岛明月蓝海生物科技有限公司，山东青岛 266400
3.赛默飞世尔科技（中国）有限公司，上海201206

收稿日期:2013-05-31 出版日期:2013-11-01 发布日期:2013-08-13
通讯作者: 毛志泉，Tel：0538-8241984；E-mail：mzhiquan@sdau.edu.cn
作者简介:尹承苗，E-mail：yinchengmiao@163.com
基金资助:
现代农业产业技术体系建设专项资金（CARS-28）、山东省农业重大应用技术创新课题、教育部长江学者和创新团队发展计划（IRT1155）

A New Method for Analysis of Phenolic Acids in the Soil —Soil from Replanted Apple Orchards was Investigated

YIN Cheng-Miao-1, WANG Gong-Shuai-1, LI Yuan-Yuan-2, CHE Jin-Shui-3, SHEN Xiang-1, CHEN Xue-Sen-1, MAO Zhi-Quan-1, WU Shu-Jing-1

1.College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology，Taian 271018, Shandong
2.Qingdao Bright Moon Bluesea Bio-Tech Co, LTD, Qingdao 266400, Shandong
3.Thermo Fisher Scientific, Shanghai 201206

Received:2013-05-31 Online:2013-11-01 Published:2013-08-13

摘要/Abstract

摘要： 【目的】建立一种以ASE-HPLC法为基础的快速、高效测定果园土壤酚酸类物质含量的新方法。【方法】以苹果特征酚酸类物质根皮苷为例，采用加速溶剂提取法（accelerated solvent extraction, ASE）和高效液相色谱法(high performance liquid chromatography, HPLC)相结合的测定方法，对萃取溶剂、萃取温度、压力和循环数等参数进行优化，寻找ASE法提取酚酸类物质的最佳工艺条件。【结果】ASE法提取苹果园土壤酚酸类物质的最佳工艺条件为：先以无水乙醇为萃取溶剂，再以甲醇为萃取溶剂，提取温度为120℃，压强为10.3 MPa，循环2次，每次静态提取时间为5 min，吹扫体积为60%，吹扫时间为90 s。【结论】该方法样品处理简单，具有良好的重现性和线性，相关性系数均达到0.99，回收率在83%—98%之间，检测限为1.3×10-4—2.5×10-2 μg•mL-1。ASE-HPLC法是一种简便、快速和高效测定土壤酚酸含量的新方法，具有推广应用价值。

关键词: 连作苹果园 , 酚酸类物质 , 加速溶剂萃取法 , 高效液相色谱法

Abstract: 【Objective】To establish a new protocol based on the ASE-HPLC method, which could determine the content of phenolic acids in orchard soil rapidly and efficiently.【Method】Phlorizin was set as an sample for analysis, which is one of the characteristic phenolic acids in apple orchard soil. Accelerated solvent extraction (ASE) combined with high performance liquid chromatography (HPLC) was used to assess it. Specifically, the parameters of extraction solvent, extraction temperature, pressure and number of cycles were optimized, aiming at exploring the optimal conditions of ASE extraction for phenolic acids.【Result】The optimal conditions of ASE extraction for phenolic acids were as follows: Ethanol as extraction solvent initially, and methanol was the subsequent extraction solvent，extraction temperature at 120℃, a pressure of 10.3 MPa, 2 cycles, every 5 min static time, 60% purge volume, and purge time for 90 s. 【Conclusion】The process was more simple, and with good reproducibility and linearity, correlation coefficient up to 0.99, recovery efficiency between 83% and 98%, detection limit to the amount of 1.3 × 10-4 -2.5 × 10-2 μg•mL-1. ASE-HPLC is an accurate and rapid new protocol to mensurate phenolic acids, which is an improved and practical method.

Key words: replanted apple orchards , phenolic acids , accelerated solvent extraction , performance liquid chromatography

尹承苗1, 王功帅1, 李园园2, 车金水3, 沈向1, 陈学森1, 毛志泉1, 吴树敬1. 一种分析土壤中酚酸类物质含量的新方法—以连作苹果园土壤为试材[J]. 中国农业科学, 2013, 46(21): 4612-4619.

YIN Cheng-Miao-1, WANG Gong-Shuai-1, LI Yuan-Yuan-2, CHE Jin-Shui-3, SHEN Xiang-1, CHEN Xue-Sen-1, MAO Zhi-Quan-1, WU Shu-Jing-1. A New Method for Analysis of Phenolic Acids in the Soil —Soil from Replanted Apple Orchards was Investigated[J]. Scientia Agricultura Sinica, 2013, 46(21): 4612-4619.

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一种分析土壤中酚酸类物质含量的新方法—以连作苹果园土壤为试材

A New Method for Analysis of Phenolic Acids in the Soil —Soil from Replanted Apple Orchards was Investigated

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