禽源奇异变形杆菌超广谱β-内酰胺酶基因的分子特征

doi:10.3864/j.issn.0578-1752.2013.07.017

摘要/Abstract

摘要： 【目的】研究禽源奇异变形杆菌携带超广谱β-内胺酰酶基因的亚型和blaCTX-M基因的上下游环境。【方法】对分离、鉴定的奇异变形杆菌进行药物敏感性试验、ESBLs产酶确证试验、耐药基因检测和接合试验；利用PCR定位技术（PCR mapping）分析blaCTX-M的基因环境。【结果】ESBLs确证试验表明，21株禽源奇异变形杆菌有10株是阳性表型。PCR扩增和测序结果表明，最常见的ESBL基因是blaCTX-M-14 (n=6) , blaOXA-1 (n=6), 其次是blaCTX-M-65 (n=4)，同时也检测到了blaOXA-10 (n=1)，没有检测到blaSHV。序列分析表明，禽源奇异变形杆菌携带blaCTX-M的上游普遍存在ISEcp1B，下游均为IS903D。【结论】首次在禽源奇异变形杆菌中检测到了blaCTX-M-65，CTX-M型超广谱β-内酰胺酶基因在禽源奇异变形杆菌中已不在少见。

关键词: 奇异变形杆菌 , ESBLs , blaCTX-M , 插入序列元件 , 家禽

Abstract: 【Objective】This experiment was conducted to explore the genotpye of extended-spectrum β-lactamases (ESBLs) gene and the genetic environment of blaCTX-M carried by Proteus mirabilis. 【Method】The isolates were characterized by isolation and identification, antimicrobial susceptibility testing, confirmation of ESBLs, resistant genes screening, and conjugation experiments. PCR mapping was performed to investigate the genetic environment of blaCTX-M. 【Result】 Among the 21 P. mirabilis clinical isolates from poultry, 10 (47.6%) isolates exhibited extended-spectrum β-lactamase (ESBL) phenotypes. The most common types of ESBL gene screened by PCR and sequencing experiments were blaCTX-M-14 (n=6) , blaOXA-1 (n=6), followed by blaCTX-M-65 (n=4). The blaOXA-10 (n=1) was also detected, while blaSHV was not detected. Nucleotide sequence analysis revealed that insertion sequence ISEcp1B elements present in the upstream region of blaCTX-M genes in all ESBL-producing P. mirabilis, and IS903D was also detected in downstream of CTX-M-9 group genes.【Conclusion】This is the first report the blaCTX-M-65 gene was found in P. mirabilis in China, and the results showed that CTX-M-tpye ESBL in P. mirabilis is no longer an unusual phenomenon in poultry origins.

Key words: Proteus mirabilis , extended-spectrum β-Lactamases , blaCTX-M gene , insertion sequence element , poultry

潘玉善, 苑丽, 吴华, 刘建华, 刘珍珍, 赵宜双, 胡功政. 禽源奇异变形杆菌超广谱β-内酰胺酶基因的分子特征[J]. 中国农业科学, 2013, 46(7): 1463-1469.

PAN Yu-Shan, YUAN Li, WU Hua, LIU Jian-Hua, LIU Zhen-Zhen, ZHAO Yi-Shuang, HU Gong-Zheng. Molecular Characteristics of Extended-Spectrum β-Lactamases in Clinical Isolates of Proteus mirabilis from Poultry[J]. Scientia Agricultura Sinica, 2013, 46(7): 1463-1469.

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