中国农业科学 ›› 2011, Vol. 44 ›› Issue (14): 2900-2908 .doi: 10.3864/j.issn.0578-1752.2011.14.006

• 植物保护 • 上一篇    下一篇

10个小麦新品种(系)抗小麦叶锈性评价

师丽红;张 娜;胡亚亚;杨文香;刘大群   

  1. 河北农业大学植物保护学院植物病理系分子植物病理学实验室/河北省农作物病虫害生物防治工程技术研究中心
  • 收稿日期:2010-11-26 修回日期:2010-12-15 出版日期:2011-07-15 发布日期:2011-07-15
  • 通讯作者: 刘大群

Evaluation of Wheat Leaf Rust Resistance of 10 New Wheat Cultivars (Lines)

SHI Li-hong; ZHANG Na; HU Ya-ya; YANG Wen-xiang; LIU Da-qun   

  1. 河北农业大学植物保护学院植物病理系分子植物病理学实验室/河北省农作物病虫害生物防治工程技术研究中心
  • Received:2010-11-26 Revised:2010-12-15 Online:2011-07-15 Published:2011-07-15

摘要: 【目的】明确小麦新品种(系)河农5290、河农58-3、河农825、河农826、河农827、河农6049、河农6251、河农6425、河农7106和河农9206的抗叶锈性,确定其应用潜力,为合理推广使用这些品种(系)提供依据。【方法】采用16个具有鉴别能力的小麦叶锈菌株对测试的10个材料进行苗期抗性鉴定和基因推导;选用其中6个菌株对其进行田间成株期抗叶锈性鉴定和基因推导,同时,结合使用已报道的15个抗叶锈病基因稳定的分子标记对测试材料进行抗叶锈基因的分子检测。【结果】河农6425和河农9206含有抗病基因Lr1和Lr26,河农825、河农826、河农827、河农6049和河农6251含有Lr26。河农58-3、河农5290、河农6251和河农825对大部分供试菌株表现抗性,可能含有测试基因以外的或未知抗叶锈基因。【结论】河农58-3、河农5290和河农6251具有抗叶锈病应用潜力。

关键词: 小麦叶锈病, 基因推导, 抗病性鉴定, 分子辅助选择

Abstract: 【Objective】 The objective of this study was to investigate the leaf rust resistance of 10 new wheat cultivars (lines) including Henong 5290, Henong 58-3, Henong 825, Henong 826, Henong 827, Henong 6049, Henong 6251, Henong 6425, Henong 7106 and Henong 9206. 【Method】Sixteen Puccinia triticina strains with different virulence patterns were used for seedling genes postulation and 6 strains of them for the test at adult stage. A series of STS and SCAR markers linked to 15 wheat leaf rust resistance genes were used for marker assisted selection of these tested cultivars (lines).【Result】Lr1 was found in cultivars Henong 6425 and Henong 9206. Lr26 was detected in 7 cultivars including Henong 825, Henong 826, Henong 827, Henong 6049, Henong 6251, Henong 6425 and Henong 9206. Henong 5290, Henong 58-3, Henong 825 and Henong 6251 were postulated containning other or unknown leaf rust resistance genes, and they were resistant to most of the P. triticina strains tested. 【Conclusion】 Henong 58-3, Henong 5290, and Henong 6251 showed potential use in wheat breeding for leaf rust resistance.

Key words: wheat leaf rust, genes postulation, resistance identification, marker-assisted selection