关键词: 猪')">猪, 标记辅助选择, 背膘厚, 生长性状, 多基因标记

Abstract: 【Objective】The objective of this study is to detect and analyze the polymorphiams of the candidate genes (IGF2, MC4R, JHDM1A and TEF-1) with growth and backfat thickness traits in the Large White pigs population.【Method】The polymorphism of these genes was identified and detected by PCR-RFLP, the SPSS software was used to analyze the association signal gene marker and polygene markers with the growth and backfat thickness traits.【Result】The analysis of single gene marker indicated that the polymorphism of IGF2 was significantly associated with backfat at the loin (P＜0.05), the excellent genotype was AA which displayed thinner backfat thickness than AG (0.541 cm) and GG(0.629 cm) genotypes. The polymorphism of MC4R was not significantly associated with the analyzed traits. The polymorphisms of JHDM1A was associated with the 100 kg backfat at 100 kg (P＜0.05), TEF-1 suggestively significantly associated with backfat at the loin at 100 kg, and CC and GG were excellent genotypes, respectively. And TEF-1 was also associated with the age at 100 kg, and AG was the excellent genotype.【Conclusion】Analysis of the effect of polygene markers showed that the genetic effect consistent with the single gene marker. The combination genotypes AGCCGG and AACCGG of IGF2, TEF-1 and JHDM1A were excellent combination genotypes for 100 kg backfat at the loin and can be used as molecular markers of backfat thinkness.

Key words: pigs')">pigs, MAS, backfat thickness, growth traits, polygene markers