中国农业科学 ›› 2010, Vol. 43 ›› Issue (18): 3809-3816 .doi: 10.3864/j.issn.0578-1752.2010.18.015

• 园艺 • 上一篇    下一篇

香蕉枯萎病致病菌筛选及致病菌浓度对香蕉枯萎病的影响

何欣,黄启为,杨兴明,冉炜,徐阳春,沈标,沈其荣   

  1. (南京农业大学资源与环境科学学院/江苏省固体有机废弃物资源化利用高技术研究重点实验室)
  • 收稿日期:2010-03-17 修回日期:2010-05-21 出版日期:2010-09-15 发布日期:2010-09-15
  • 通讯作者: 杨兴明

Screening and Identification of Pathogen Causing Banana Fusarium Wilt and the Relationship Between Spore Suspension Concentration and the Incidence Rate

HE Xin, HUANG Qi-wei, YANG Xing-ming, RAN Wei, XU Yang-chun, SHEN Biao, SHEN Qi-rong
  

  1. (南京农业大学资源与环境科学学院/江苏省固体有机废弃物资源化利用高技术研究重点实验室)
  • Received:2010-03-17 Revised:2010-05-21 Online:2010-09-15 Published:2010-09-15
  • Contact: YANG Xing-ming

摘要:

【Objective】 In order to provide a scientific basis for the field diagnoses of Fusarium wilt of banana, it is very important to screen and identify the pathogen which causes banana Fusarium wilt and to investigate the relationship between the spore suspension concentration and the disease incidence rate. 【Method】 A lab-screening and pot experiment was used to identify the pathogen. 【Result】 The pathogen isolated from the pseudo-crown of diseased banana showed a high pathogenicity in a pot experiment in which the disease incidence was 90% and mortality rate was as high as 70% 35 days after inoculation. A close relationship between the amount of inocula in soil and the disease incidence rate of banana wilt was found and the minimal concentration that induced the banana Fusarium wilt was 103 CFU /g soil. Disease incidence increased with the increase of inocula density in soil. Until the inocula density attained a certain degree (105 CFU/g), the disease incidence would no longer increase. The numbers of pathogen in rhizosphere soil were 1.15 to 2.06 times higher than those in bulk soil in different treatments. A pot experiment was carried out to investigate the effects of a new bioorganic fertilizer (BIO) on banana plant growth and the suppression on the disease. It was found that the BIO bio-organic fertilizer contributed to the growth-promoting of banana plants and to the low occurences of fusarium-wilt disease. 【Conclusion】 It was related to background number of the pathogen in diseased soil that the banana Fusarium wilt occurred or not, while the disease incidence was mainly determined by the number of the pathogen in plant rhizosphere soil. These results would be served for the diagnosis of banana wilt.

关键词: 香蕉枯萎病, 病原菌, 孢子浓度, 发病程度

Abstract: Abstract:【OBJECTIVE】Banana Fusarium wilt was one of the most important soilborne diseases which affected the normal development of the banana industry. In order to prevent the spread of the wilt disease and protect the safety and health of the banana production, it is very important to screen and identify the pathogen which caused banana Fusarium wilt and investigate the relationship between the spore suspension concentration and the disease incidence rate;【METHOD】Through lab-screening and pot experiment to identificate the pathogen;【RESULTS】The pathogen isolated from the department of pseudo-crown of diseased banana showed a high pathogenicity from a pot experiment in which the disease incidence was 90% and mortality rate was as high as 70% on 35 days after inoculation. A close relationship between the amount of inocula in soil and the disease incidence rate of banana wilt was found and the minimal concentration that induced the banana Fusarium wilt was 103cfu /g soil. Disease incidence was increased with the increase of inocula density in soil. Until the inocula density attained to a certain degree (105cfu/g), the disease incidence would no longer increase. The numbers of pathogen in rhizosphere soil were 1.15 to 2.06 times higher than those in bulk soil in different treatments. 【CONCLUSION】It was related to background number of the pathogen in diseased soil that the banana fusarium wilt occurred or not, while the disease incidence was mainly determined by the number of the pathogen in plant rhizosphere soil. These results would be served for the diagnosis of banana wilt.

Key words: banana Fusarium wilt, pathogen, spore suspension concentration, disease severity