关键词: 玫瑰')">玫瑰, 自交亲和性, 荧光显微观察

Abstract:

【Objective】 The aim of this experiment is to reveal the self-compatibility of Rosa rugosa Thunb. It is meaningful to improve the theory and techniques of breeding ornamental plant in Rosa especially when Rosa rugosa Thunb. used as maternal parent. 【Method】Analysis of the rate of fertile fruits and average seed number combined with fluorescent microscope observation of pollen tubes was employed in this study. 【Result】 No fruit was produced and the pollen tubes were arrested at the upper one third of the styles when all the plant materials were self-pollinated on anthesis and 4 days before anthesis. The self-compatibility of Rosa rugosa Thunb. could be improved when they were pollinated before anthesis, and the effect of self-pollination on 2 days before anthesis was the best. For the rate of fertile fruit and average seed number of Rosa rugosa Thunb., significant and positive relationship was found between self-pollination in bud period and open pollination. 【Conclusion】 Rosa rugosa Thunb. is completely gametophytic self-incompatible. The self-compatibility of Rosa rugosa Thunb. can be improved by pollination in bud period, but the effect varies with the change of pollination periods and cultivars.

Key words: Rosa rugosa Thunb.')">Rosa rugosa Thunb., self-compatibility, fluorescent microscope observation