关键词: 白芍, 中性粒细胞, 磷酸二酯酶, 呼吸爆发, 弹性蛋白酶

Abstract:

【Objective】 The objective of this experiment is to study the influence of Baishao (Paeonia lactiflora) preparation on respiratory burst and elastase release of neutrophils with porcine neutrophils as the target cells. 【Method】 after the separation and purification of neutrophils, respiratory burst of neutrophils was detected with cytochrome C reduction method, elastase release was indicated with the substrate reduction of elastase, and phosphodiesterase (PDE) activity was calculated by cAMP change examined with HPLC before and after the reaction. 【Result】 Baishao preparation could significantly inhibit neutrophils respiratory burst dose dependently (P＜0.001), the inhibition ratios of three different concentrations were 33.15%, 45.96% and 62.22%, respectively; the adjustment of Baishao preparation on elastase release was duplicate, having inhibition in low concentration, inhibition ratio was 23.22%, while having promotion in high concentration. Baishao preparation could obviously inhibit PDE activity in neutrophils, and the inhibition ratios of low and high concentration were 13.24% and 25.87% (P＜0.01), respectively. Analysis of HPLC finger print showed that there were five ingredients in Baishao preparation. 【Conclusion】 Baishao preparation has significant inhibition on respiratory burst of neutrophils, and has obvious inhibition on elastase release at low concentration, the mechanism of which possibly has a close relation with the inhibition on PDE activity.

Key words: baishao, neutrophils, phosphodiesterase, respiratory burst, elastase