中国农业科学 ›› 2009, Vol. 42 ›› Issue (5): 1639-1645 .doi: 10.3864/j.issn.0578-1752.2009.05.017

• 植物保护 • 上一篇    下一篇

中红侧沟茧蜂非典型气味受体的克隆及组织特异性表达

  

  1. 中国农业科学研究院植物保护研究所/植物病虫害生物学国家重点实验室

  • 收稿日期:2008-05-29 修回日期:2008-07-07 出版日期:2009-05-10 发布日期:2009-05-10
  • 通讯作者: 郭予元

Gene Cloning and Tissue-Specific Expression of an Olfactory Receptor in Microplitis Mediator (Hymenoptera: Braconidae)

  

  1. 中国农业科学研究院植物保护研究所/植物病虫害生物学国家重点实验室

  • Received:2008-05-29 Revised:2008-07-07 Online:2009-05-10 Published:2009-05-10
  • Contact: GUO Yu-yuan

摘要:

【目的】研究中红侧沟茧蜂Microplitis mediator非典型气味受体的表达谱。【方法】使用RT-PCR法克隆得到中红侧沟茧蜂非典型气味受体基因序列。使用实时荧光定量PCR法研究其表达谱。【结果】克隆得到中红侧沟茧蜂非典型气味受体全长基因。该基因在触角中特异性表达。在雌虫触角中羽化当天达到最大表达量,雄虫触角中在羽化前d阶段达到最大表达量。【结论】该非典型气味受体在中红侧沟茧蜂触角中特异存在。

关键词: 中红侧沟茧蜂, 嗅觉, 非典型气味受体, 组织表达

Abstract:

【Objective】 The expression of an olfactory receptor of Microplitis mediator was studied. 【Method】 The olfactory receptor gene of Microplitis mediator was cloned using RT-PCR. And its tissue-specific expression and quantitative changes at different developmental stages were detected using real-time PCR. 【Result】 A gene encoding a novel olfactory receptor, which is antenna- specific, was isolated from Microplitis mediator (Hymenoptera: Braconidae). Its expression levels reached the maximum amount on the emergence day of female and reached the maximum amount at the d stage before emergence of male. 【Conclusion】This receptor is antenna- specific in Microplitis mediator.

Key words: Microplitis mediator, olfaction, olfactory receptor, expression pattern