中国农业科学 ›› 2009, Vol. 42 ›› Issue (1): 283-289 .doi: 10.3864/j.issn.0578-1752.2009.01.036

• 畜牧·资源昆虫 • 上一篇    下一篇

猪POU1F1第一内含子单核苷酸多态性和生长性状相关性研究

  

  1. 中国农业科学院北京畜牧兽医研究所
  • 收稿日期:2008-01-28 修回日期:2008-04-27 出版日期:2009-01-10 发布日期:2009-01-10
  • 通讯作者: 郑友民

Relationship of Single Nucleotide Polymorphism of Intron 1 in Procine POU1F1 and the Growth Trait

  

  1. 中国农业科学院北京畜牧兽医研究所
  • Received:2008-01-28 Revised:2008-04-27 Online:2009-01-10 Published:2009-01-10
  • Contact: ZHENG You-min

摘要:

【目的】寻找猪POU1F1多态位点和基因表达量之间可能存在的相关性。【方法】以大白猪(英系)和中畜黑猪为试验材料,对POU1F1基因第一内含子多态性进行PCR-SSCP检测分析,同时提取垂体总RNA,对POU1F1、GH、PRL、TSH-?的mRNA进行实时定量荧光PCR检测。【结果】发现一个位于第一内含子1515位点的突变和猪屠宰前日增重相关系数为R=-0.6(P<0.05),且该位点的替换和屠宰前GH的表达量存在显著相关(R=0.483,P<0.05);【结论】T→C的替换将导致GH的mRNA表达量升高,在生长性状上表现为屠宰前日增重降低。

关键词: POU1F1, PCR-SSCP, 多态性, SNP位点

Abstract:

【Objective】 The relationship between porcine DNA polymorphisms of POU1F1 and mRNA expression was studied. 【Method】 The intron 1, a partial regional polymorphisms of porcine POU1F1 of two breeds of Yorkshare and Zhongxu black, were investigated by PCR-SSCP. Based on the total RNA contracted from animals pituitary, the mRNA quantities of POU1F1, GH, PRL, and TSH-? were masured by RQ-PCR. 【Result】 It was found that a SNP of T→C in intron 1 (site 1515bp), markedly correlated with the daily weight gain (R=-0.6, P<0.05) and the expression of GH (P<0.05) before slaughtering. 【Conclusion】The SNP of T→C led to the increase of the GH expression; But the daily weight gain decreasd in the growth trait before slaughtering.

Key words: POU1F1, PCR-SSCP, polymorphisms, SNP