The rice white tip nematode (RWTN) Aphelenchoides besseyi secretes effectors that manipulate host plant cells to facilitate successful parasitism and sustain infection.  Although the number of identified RWTN effectors remains limited, their mechanisms of interaction with host plants are largely unknown.  Profilins (PFNs) function as molecular hubs that regulate complex interaction networks.  To advance understanding of PFN3 in plant-parasitic nematodes, we identified an effector from A. besseyi, designated AbPFN3.  AbPFN3 is transcriptionally upregulated during the juvenile stage of the nematode, and in situ hybridization localized its expression to the esophageal glands.  Three rice (Oryza sativa) proteins, ADP/ATP carrier protein 1 (OsAAC1), B-cell receptor-associated protein 31 (OsBAP31) and Small Auxin Up RNA 50 (OsSAUR50), were identified as interactors of AbPFN3, with interactions occurring in distinct cellular compartments, including the endoplasmic reticulum, cytoplasm, and plasma membrane.  Transgenic analyses revealed that AbPFN3 expression significantly increased plant height and upregulated AAC1 and BAP31, while downregulating RGA2 and SAUR50.  This study characterizes AbPFN3 as a novel effector secreted by A. besseyi that interacts with multiple host proteins, highlighting its potential role in modulating host defense responses and cell development processes.