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Linkage and association mapping of wild soybean (Glycine soja) seeds germinating under salt stress
SHI Mei-qi, LIAO Xi-liang, YE Qian, ZHANG Wei, LI Ya-kai, Javaid Akhter BHAT, KAN Gui-zhen, YU De-yue
2022, 21 (10): 2833-2847.   DOI: 10.1016/j.jia.2022.07.031
Abstract203)      PDF in ScienceDirect      

Salinity threatens soybean germination, growth and production.  The germination stage is a key period in the life of soybean.  Wild soybean contains many genes related to stress resistance that are valuable resources for the genetic improvement of soybean.  To identify the genetic loci of wild soybean that are active during seed germination under salt stress, two populations, a soybean interspecific hybrid population comprising 142 lines and a natural population comprising 121 wild soybean accessions, were screened for three germination-related traits in this study.  By using single-nucleotide polymorphism (SNP) markers with three salt tolerance indices, 25 quantitative trait loci (QTLs), 21 significant SNPs (–log10(P)≥4.0) and 24 potential SNPs (3.5<–log10(P)<4.0) were detected by linkage mapping and a genome-wide association study (GWAS) in two environments.  The key genetic region was identified based on these SNPs and QTLs.  According to the gene functional annotations of the W05 genome and salt-induced gene expression qRT-PCR analysis, GsAKR1 was selected as a candidate gene that responded to salt stress at the germination stage in the wild soybean.  These results could contribute to determining the genetic networks of salt tolerance in wild soybean and will be helpful for molecular marker-assisted selection in the breeding of salt-tolerant soybean.

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Mapping the fallowed area of paddy fields on Sanjiang Plain of Northeast China to assist water security assessments
LUO Chong, LIU Huan-jun, FU Qiang, GUAN Hai-xiang, YE Qiang, ZHANG Xin-le, KONG Fan-chang
2020, 19 (7): 1885-1896.   DOI: 10.1016/S2095-3119(19)62871-6
Abstract147)      PDF in ScienceDirect      
Rice growth requires a large amount of water, and planting rice will increase the contradiction between supply and demand of water resources.  Paddy field fallowing is important for the sustainable development of an agricultural region, but it remains a great challenge to accurately and quickly monitor the extent and area of fallowed paddy fields.  Paddy fields have unique physical features associated with paddy rice during the flooding and transplanting phases.  By comparing the differences in phenology before and after paddy field fallowing, we proposed a phenology-based fallowed paddy field mapping algorithm.  We used the Google Earth Engine (GEE) cloud computing platform and Landsat 8 images to extract the fallowed paddy field area on Sanjiang Plain of China in 2018.  The results indicated that the Landsat8, GEE, and phenology-based fallowed paddy field mapping algorithm can effectively support the mapping of fallowed paddy fields on Sanjiang Plain of China.  Based on remote sensing monitoring, the total fallowed paddy field area of Sanjiang Plain is 91 543 ha.  The resultant fallowed paddy field map is of high accuracy, with a producer (user) accuracy of 83% (81%), based on validation using ground-truth samples.  The Landsat-based map also exhibits high consistency with the agricultural statistical data.  We estimated that paddy field fallowing reduced irrigation water by 384–521 million cubic meters on Sanjiang Plain in 2018.  The research results can support subsidization grants for fallowed paddy fields, the evaluation of fallowed paddy field effects and improvement in subsequent fallowed paddy field policy in the future. 
 
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