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TaFLZ54D enhances salt stress tolerance in wheat by interacting with TaSGT1 and TaPP2C

Yuxiang Qin, Bao Zhang, Shoufu Cui, Xiaochun Qin, Genying Li

2025, 24 (3): 1017-1029. DOI: 10.1016/j.jia.2023.09.018

Abstract （87） PDF in ScienceDirect

FCS-like zinc finger (FLZ) gene family members are C2-C2 zinc finger proteins that take part in seed dormancy, resistance to Myzus persicae 1, sucrose signaling and abiotic stresse tolerance. However, their functions, especially the molecular mechanism through which FLZs function, are not well understood. In this study, we characterized 120 FLZs in wheat and revealed the function and mechanism of TaFLZ54D increasing salt stress tolerance in transgenic wheat. Expression analysis demonstrated that TaFLZ54D can be induced by NaCl treatment and it had the highest expression level under NaCl treatment among the 120 FLZs. Over-expression of TaFLZ54D increased wheat salt stress tolerance and the transgenic plants had higher levels of superoxide dismutase (SOD) and peroxidase (POD) activities and soluble sugar content, but a lower Na⁺/K⁺ ratio and malondialdehyde (MDA) content than the wild type (WT) plants. Potassium ion transmembrane transporters and serine/threonine kinase inhibitor proteins showed differential expression between TaFLZ54D transgenic wheat and the WT. Yeast two hybrid and luciferase complementation assays revealed that TaSGT1 and TaPP2C are the proteins that interact directly with TaFLZ54D. In summary, TaFLZ54D enhances salt stress tolerance through interaction with TaSGT1 and TaPP2C to reduce Na⁺absorption and mitigate oxidative stress. The interaction between TaFLZ54D and TaSGT1, as well as TaPP2C indicated a link between salt stress tolerance of TaFLZ54D and the ubiquitin-mediated degradation of negative regulatory proteins.

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Investigation of Aegilops umbellulata for stripe rust resistance, heading date, and the contents of iron, zinc, and gluten protein

SONG Zhong-ping, ZUO Yuan-yuan, XIANG Qin, LI Wen-jia, LI Jian, LIU Gang, DAI Shou-fen, YAN Ze-hong

2023, 22 (4): 1258-1265. DOI: 10.1016/j.jia.2022.08.014

Abstract （303） PDF in ScienceDirect

Aegilops umbellulata (UU) is a wheat wild relative that has potential use in the genetic improvement of wheat. In this study, 46 Ae. umbellulata accessions were investigated for stripe rust resistance, heading date (HD), and the contents of iron (Fe), zinc (Zn), and seed gluten proteins. Forty-two of the accessions were classified as resistant to stripe rust, while the other four accessions were classified as susceptible to stripe rust in four environments. The average HD of Ae. umbellulata was significantly longer than that of three common wheat cultivars (180.9 d vs. 137.0 d), with the exception of PI226500 (138.9 d). The Ae. umbellulata accessions also showed high variability in Fe (69.74–348.09 mg kg^–1) and Zn (49.83–101.65 mg kg^–1) contents. Three accessions (viz., PI542362, PI542363, and PI554399) showed relatively higher Fe (230.96–348.09 mg kg^–1) and Zn (92.46–101.65 mg kg^–1) contents than the others. The Fe content of Ae. umbellulata was similar to those of Ae. comosa and Ae. markgrafii but higher than those of Ae. tauschii and common wheat. Aegilops umbellulata showed a higher Zn content than Ae. tauschii, Ae. comosa, and common wheat, but a lower content than Ae. markgrafii. Furthermore, Ae. umbellulata had the highest proportion of γ-gliadin among all the species investigated (Ae. umbellulata vs. other species=mean 72.11% vs. 49.37%; range: 55.33–86.99% vs. 29.60–67.91%). These results demonstrated that Ae. umbellulata exhibits great diversity in the investigated traits, so it can provide a potential gene pool for the genetic improvement of these traits in wheat.

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Copyright © Journal of Integrative Agriculture
Sponsored by Chinese Academy of Agricultural Sciences (CAAS)
Co-sponsored by China Association of Agricultural Science Societies (CAASS)
Publishing Service by Elsevier B.V.on behalf of KeAi Communications Co. Ltd.
Full text available online at ScienceDirect

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