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Silicon impacts on soil microflora under Ralstonia Solanacearum inoculation
LIN Wei-peng, JIANG Ni-hao, PENG Li, FAN Xue-ying, GAO Yang, WANG Guo-ping, CAI Kun-zheng
2020, 19 (1): 251-264.   DOI: 10.1016/S2095-3119(18)62122-7
Abstract148)      PDF in ScienceDirect      
Silicon (Si) can increase plant resistance against bacterial wilt caused by Ralstonia solanacearum and enhance plant immune response.  However, whether Si alleviates soil-borne disease stress through altering soil microbial community component and diversity is not clear.  In this study, effects of Si application under R. solanacearum inoculation with or without plant on soil bacterial and fungal communities were investigated through high-throughput pyrosequencing technique.  The results showed that Si addition significantly reduced bacterial wilt incidence.  However, Si did not reduce the amount of R. solanacearum in rhizosphere soil.  Principal components analysis showed that soil microbial community composition was strongly influenced by Si addition.  Total 63.7% bacterial operational taxonomic units (OTUs) and 43.8% fungal OTUs were regulated by Si addition regardless of the presence of tomato plants, indicating the independent effects of Si on soil microbial community.  Si-added soil harbored a lower abundance of Fusarium, Pseudomonas, and Faecalibacterium.  Our finding further demonstrated that exogenous Si could significantly influence soil microbial community component, and this may provide additional insight into the mechanism of Si-enhanced plant resistance against soil-borne pathogens.
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Molecular, serological and biological characterization of a novel Apple stem pitting virus strain from a local pear variety grown in China
LI Liu, ZHENG Meng-meng, MA Xiao-fang, LI Yuan-jun, LI Qing-yu, WANG Guo-ping, HONG Ni
2019, 18 (11): 2549-2560.   DOI: 10.1016/S2095-3119(19)62636-5
Abstract127)      PDF in ScienceDirect      
Apple stem pitting virus (ASPV) is an important causal agent of pear diseases.  Nowadays, the infection status and molecular characteristics of the virus in old pear trees have never been investigated.  In this study, we provide the first complete genome sequence of an ASPV isolate LYC from an over 300-year-old tree of a local Pyrus bretschneideri cultivar ‘Chili’ specifically grown at Laiyang area in China.  ASPV-LYC possesses a chimeric genome consisting of 9 273 nucleotides excluding a poly(A) tail at its 3´ end and harboring a recombination region in its open reading frame (ORF1) with Aurora-1 and KL9 identified as the major and minor parents.  Western blot analysis with antisera against recombinant coat proteins (CPs) of three ASPV isolates from pear indicates that ASPV-LYC is serologically related to these ASPV isolates, but with differential activities.  Further biological tests on indicator plants of Pyronia veitchii show that ASPV-LYC can induce serious leaf and stem symptoms as other ASPV isolates.  The results provide an important information for understanding molecular evolution of ASPV and suggest a need to prevent dissemination of the isolate among pear trees.
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Estimating light interception using the color attributes of digital images of cotton canopies
XUE Hui-yun, HAN Ying-chun, LI Ya-bing, WANG Guo-ping, FENG Lu, FAN Zheng-yi, DU Wen-li, YANG Bei-fang, MAO Shu-chun
2017, 16 (07): 1474-1485.   DOI: 10.1016/S2095-3119(16)61542-3
Abstract721)      PDF in ScienceDirect      
    Crop growth and yield depend on canopy light interception (LI). To identify a low-cost and relatively efficient index for measuring LI, several color attributes of red-green-blue (RGB), hue-saturation-intensity (HSI), hue-saturation-value (HSV) color models and the component values of color attributes in the RGB color model were investigated using digital images at six cotton plant population densities in 2012–2014. The results showed that the LI values followed downward quadratic curves after planting. The red (R), green (G) and blue (B) values varied greatly over the years, in accordance with Cai’s research demonstrating that the RGB model is affected by outside light. Quadratic curves were fit to these color attributes at six plant population densities. Additionally, linear regressions of LI on every color attribute revealed that the hue (H) values in HSI and HSV were significantly linearly correlated with LI with a determination coefficient (R2)≥0.89 and a root mean square error (RMSE)=0.05. Thus, the H values in the HSI and HSV models could be used to measure LI, and this hypothesis was validated. The H values are new indexes for quantitatively estimating the LI of heterogeneous crop canopies, which will provide a theoretical basis for optimizing the crop canopy structure. However, further research should be conducted in other crops and under other growing and environmental conditions to verify this finding.
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Effects of plant density on cotton yield components and quality
ZHI Xiao-yu, HAN Ying-chun, LI Ya-bing, WANG Guo-ping, DU Wen-li, LI Xiao-xin, MAO Shu-chun, FENG Lu
2016, 15 (7): 1469-1479.   DOI: 10.1016/S2095-3119(15)61174-1
Abstract1673)      PDF in ScienceDirect      
     Yield and fiber quality of cotton even varies within locules in a boll, but it is not clear how yield components and quality parameters are altered across seed positions of a locule (SPL). A field experiment was arranged in a split plot design with transgenic insect resistant Bt (Bacillus thuringiensis) cotton hybrid cultivar CRI75 and conventional cultivar SCRC28 as the main plots, and three plant densities (15 000, 51 000 and 87 000 plants ha–1) as the subplots in 2012 and 2013 at Anyang, Henan Province, China. Cotton was hand harvested by node and fruiting position, and then seeds of the first fruiting position bolls from nodes 6–10 were separated by SPL. The effects of plant density on lint yield, fiber quality, especially across SPL were determined. It was showed that plant densities of 51 000 and 87 000 plants ha–1 increased lint yield by 61.3 and 65.3% in 2012 and 17.8 and 15.5% in 2013 relative to low plant density (15 000 plants ha–1), however, no significant difference was observed between 51 000 and 87 000 plants ha–1. The number of bolls (boll density) increased while boll weight decreased as plant density raised, and no significant changes occured in lint percentage in 2013 but increased with plant density in 2012. The number of bolls in upper nodes and distal fruiting positions, the number of seeds per boll, seed area (SA) and seed vigor index increased with decreasing plant density. Seed area was found to be greater from the base to the middle compared to the apex of a locule. Mote frequency (MF) increased as plant density increased, and fiber quality was the best at the middle of the locule regardless of plant density. As the number of fibers per seed area is genetically determined, adjusting plant density to produce more seeds and greater seed area can be a potentially promising alternative to improve lint yield in cotton. These findings might be of great importantance to cotton breeding and filed management.
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