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MRUNet: A two-stage segmentation model for small insect targets in complex environments 

WANG Fu-kuan, HUANG Yi-qi, HUANG Zhao-cheng, SHEN Hao, HUANG Cong, QIAO Xi, QIAN Wan-qiang
2023, 22 (4): 1117-1130.   DOI: 10.1016/j.jia.2022.09.004
Abstract316)      PDF in ScienceDirect      

Online automated identification of farmland pests is an important auxiliary means of pest control.  In practical applications, the online insect identification system is often unable to locate and identify the target pest accurately due to factors such as small target size, high similarity between species and complex backgrounds.  To facilitate the identification of insect larvae, a two-stage segmentation method, MRUNet was proposed in this study.  Structurally, MRUNet borrows  the practice of object detection before semantic segmentation from Mask R-CNN and then uses an improved lightweight UNet to perform the semantic segmentation.  To reliably evaluate the segmentation results of the models, statistical methods were introduced to measure the stability of the performance of the models among samples in addition to the evaluation indicators commonly used for semantic segmentation.  The experimental results showed that this two-stage image segmentation strategy is effective in dealing with small targets in complex backgrounds.  Compared with existing state-of-the-art semantic segmentation methods, MRUNet shows better stability and detail processing ability under the same conditions.  This study provides a reliable reference for the automated identification of insect larvae.

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Immunogenicity and protective efficacy of DHBV DNA vaccines expressing envelope and capsid fusion proteins in ducks delivered by attenuated Salmonella typhimurium
LIU Si-yang, JIA Ren-yong, LI Qing-qing, FENG Dai-shen, SHEN Hao-yue, YANG Cui, WANG Ming-shu, ZHU De-kang, CHEN Shun, LIU Ma-feng, ZHAO Xin-xin, YIN Zhong-qiong, JING Bo, CHENG An-chun
2018, 17 (04): 928-939.   DOI: 10.1016/S2095-3119(17)61829-X
Abstract495)      PDF in ScienceDirect      
Duck hepatitis B virus (DHBV) shares many basic characteristics with hepatitis B virus (HBV) and is an attractive model for vaccine development.  In this study, DHBV DNA vaccines were designed to express envelope and capsid fusion proteins to enhance the breadth of immune response in ducks.  Attenuated Salmonella typhimurium (SL7207) was used as a carrier and adjuvant to boost the magnitude of immune response.  Based on this strategy, novel DNA vaccines (SL7207-pVAX1-LC and SL7207-pVAX1-SC) were generated.  Growth kinetics, genetic stabilities and relative transcription levels of the L, S and C genes introduced by these vaccine strains were measured before inoculation to guarantee safety and efficacy.  The relative transcript levels of the CD4 and CD8 T genes and the antibody levels (IgY) in ducks receiving the vaccines were higher than those in single gene delivered groups.  Additionally, the copy number of covalently closed circular DNA in hepatocytes after DHBV challenge also provided evidence that our fusion vaccines could enhance the protective efficiency against DHBV infection in ducks.
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