The combined implementation of
intercropping systems and arbuscular mycorrhizal fungi (AMF) inoculation
represents a promising phytoremediation strategy for heavy metal-contaminated
farmland, providing both ecological and economic benefits. However, additional research is necessary to
understand the influence of AMF and intercropping on Cd bioavailability. This study examines the synergistic effects
of maize–soybean intercropping and AMF inoculation on crop growth, cadmium (Cd)
allocation patterns, and rhizosphere soil dynamics through comprehensive field
and pot experiments. Field trials
revealed significant yield advantages in maize–soybean intercropping systems,
with land equivalent ratios (LERs) of 1.62 (common maize) and 1.64 (sweet
maize). Intercropping decreased soybean
Cd accumulation across all tissues, notably in grains (42.8% reduction), while
maintaining maize grain Cd concentrations below China’s food safety threshold
(0.20 mg kg–1). The
metal removal equivalent ratio (MRER) achieved 1.33–1.38 in field conditions,
validating intercropping’s dual advantage in productivity and Cd
phytoextraction. Pot experiments
indicated the AMF-inoculated intercropping system (IN+A) increased maize yield
by 16.4% while reducing Cd accumulation in both crops, with grain
concentrations meeting safety standards.
Rhizosphere analysis demonstrated IN+A treatment substantially improved
soil health indicators: 34.5% reduction in bioavailable Cd, elevated pH,
decreased redox potential (Eh), and enhanced catalase activity. AMF colonization rates were 2.2–4.3 times
higher in inoculated treatments (11.5–14.0%) vs. controls
(3.2–5.3%). These results establish that
AMF-enhanced legume–cereal intercropping reduces Cd bioavailability through
soil alkalinization (pH increase) coupled with redox potential reduction, and
metal allocation plasticity redirecting Cd to root tissues. This interaction between microbial symbiosis
and plant community design stabilizes Cd in soils while maintaining crop safety
(grain Cd<0.20 mg kg–1), establishing an ecoengineering
approach for contaminated farmland remediation.