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Identification and expression analysis of the PbrMLO gene family in pear, and functional verification of PbrMLO23
GUO Bing-bing, LI Jia-ming, LIU Xing, QIAO Xin, Musana Rwalinda FABRICE, WANG Peng, ZHANG Shao-ling, WU Ju-you
2021, 20 (9): 2410-2423.   DOI: 10.1016/S2095-3119(20)63558-4
Abstract152)      PDF in ScienceDirect      
Mildew resistance locus O (MLO) is a plant-specific gene family that plays an important role in the growth and development of plants and their interactions with the environment.  However, the available information on this gene family in pear is limited.  Here, 24 PbrMLO genes were identified and divided into five subfamilies (I, II, III, IV and V).  Whole-genome duplication (WGD) and dispersed duplication contributed to the expansion of the PbrMLO family.  In addition, gene expression analysis revealed that PbrMLO genes were distributed in various pear tissues, suggesting their diverse functions.  We selected PbrMLO23 for further functional analysis.  Expression profile analysis by qRT-PCR showed that PbrMLO23 was highly expressed in pollen.  Subcellular localization analysis showed that PbrMLO23 was located on the plasma membrane.  When the expression level of PbrMLO23 was knocked down by using antisense oligonucleotides, pollen tube lengths increased, indicating that PbrMLO23 plays a functional role in inhibiting pollen tube growth.  In summary, these results provide evolutionary insight into PbrMLO and its functional characteristics and lay a foundation for further analysis of the functions of PbrMLO members in pear.
 
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Comprehensive characterization of yam tuber nutrition and medicinal quality of Dioscorea opposita and D. alata from different geographic groups in China
SHAN Nan, WANG Pu-tao, ZHU Qiang-long, SUN Jing-yu, ZHANG Hong-yu, LIU Xing-yue, CAO Tian-xu, CHEN Xin, HUANG Ying-jin, ZHOU Qing-hong
2020, 19 (11): 2839-2848.   DOI: 10.1016/S2095-3119(20)63270-1
Abstract179)      PDF in ScienceDirect      
China is an important domestication center of yams, and two main yam species of Dioscorea opposita and D. alata are commonly cultivated in China.  However, the differences of nutritional and medicinal characteristics between the two species and their subgroups remain unclear, which would greatly affect the resource conservation and commercial utilization of yams.  In this study, typical yam resources including the species of D. opposita (wild and cultivated Ruichang yam from southern China, and Tiegun yam from northern China) and two landraces of D. alata (Longyan yam and Anyuan yam from southern China) were selected as materials.  Nutritional traits and medicinal characteristics were determined and analyzed respectively.  The results showed that there was no significant differences in the content of most nutrients between D. opposita and D. alata, but most cultivated Ruichang yam of D. opposita showed higher levels of starch, soluble sugar, sucrose, and ascorbate in tuber than that in yam from D. alata.  Moreover, an UPLC-MS method was developed for identification and determination of medicinal characteristics in the two species.  The results showed that allantoin can be detected in all selected samples.  Cultivated Ruichang yam of D. opposita possessed the highest allantoin content among the tested materials, and was significantly different with that in Tiegun yam and D. alata. Dioscin was not detected in D. alata. Overall, there was little difference in nutritional composition between D. opposita and D. alata, but the medicinal quality of D. opposita was better than that of D. alata.  Due to the outstanding comprehensive quality, the local variety of cultivated Ruichang yam can be further developed and utilized.
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Influence of potato flour on dough rheological properties and quality of steamed bread
LIU Xing-li, MU Tai-hua, SUN Hong-nan, ZHANG Miao, CHEN Jing-wang
2016, 15 (11): 2666-2676.   DOI: 10.1016/S2095-3119(16)61388-6
Abstract2236)      PDF in ScienceDirect      
    It is a novel idea to make steamed bread by adding potato flour into wheat flour considering the production and nutritional factors of potato. In this study, the influence of potato flour (0–35%) on dough rheology and quality of steamed bread were investigated. Potato flour addition significantly influenced the dough rheological properties and steamed bread quality, such as increased water absorption, the maximum gaseous release height, total volume of CO2 and hardness, while decreased dough stability and specific volume of steamed bread. Moreover, correlation analysis suggested that dough height at the maximum development time, dough stability, water absorption and the phase tangent can be used for predicting the technological quality of steamed bread. Potato-wheat steamed bread had higher dietary fibre, ash content and antioxidant activity than those of wheat steamed bread. The estimated glycemic index decreased from 73.63 (0%) to 60.01 (35%). Considering the sensory evaluation, the steamed bread with 20% potato flour is acceptable. In conclusion, adding appropriate quantity of potato flour to wheat flour for steamed bread production will not only maintain the technological quality, but also can improve the nutritional value of the steamed bread.
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Assessing Maize Drought Hazard for Agricultural Areas Based on the Fuzzy Gamma Model
LIU Xing-peng, ZHANG Ji-quan, CAI Wei-ying , TONG Zhi-jun
2013, 12 (3): 532-540.   DOI: 10.1016/S2095-3119(13)60254-3
Abstract1424)      PDF in ScienceDirect      
Drought is one of the severe meteorological disasters and causes of serious losses for agricultural productions, and early assessment of drought hazard degree is critical in management of maize farming. This study proposes a novel method for assessment of maize drought hazard in different growth stages. First, the study divided the maize growth period into four critical growth stages, including seeding, elongation, tasseling, and filling. Second, maize drought causal factors were selected and the fuzzy membership function was established. Finally, the study built a fuzzy gamma model to assess maize drought hazards, and the gamma 0.93 was finally established using Monte Carlo Analysis. Performing fuzzy gamma operation with 0.93 for gamma and classifying the area yielded a map of maize drought hazards with four zones of light, moderate, severe, and extreme droughts. Using actual field collected data, seven selected samples for drought hazard degree were examined, the model output proved to be a valid tool in the assessment maize drought hazard. This model will be very useful in analyzing the spatial change of maize drought hazard and influence on yield, which is significant for drought management in major agricultural areas.
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Pathogenesis of Demyelinating Encephalopathy in Dogs with Spontaneous Acute Canine Distemper
PAN Yao-qian, LIU Xing-you, MENG Li-ping, ZHU Guang-rui, XIA Yin-ke, CHEN Jin-shan , Yoshikawa Takashi
2013, 12 (2): 334-343.   DOI: 10.1016/S2095-3119(13)60233-6
Abstract1577)      PDF in ScienceDirect      
So far, the pathogenesis of demyelination caused by canine distemper virus (CDV) in the central nervous system has remained unclear, although a lot of studies have been done extensively. To further investigate the relation of variety cells in brain to demyelination, this study was performed on 15 dogs with spontaneous acute canine distemper and 2 controls. According to anatomical relation, the brain was divided into cerebrum, cerebral stem and cerebellum. The sections with no, mild, moderate, or severe demyelinating lesions were selected respectively and stained by HE and immunohistochemistry. Immuno-localisation of CDV antigen was used to confirm CDV infection. The brain was examined for co-localisation of the CDV antigen with either an astrocyte-specific marker, glial fibrillary acidic protein (GFAP), or an oligodendrocyte-specific marker, galactocerebroside (GalC). Apoptotic cell was detected by TdT-mediated nick end-labeling assay (TUNEL). The results demonstrated that the local disturbance of blood circulation mainly included congestion, edema, thrombosis, and disseminated intravascular coagulation (DIC). The CDV neucleocapsid protein positive reaction, metabolic disorder and apoptosis of oligodendrocytes were observed in demyelinating areas. Lots of astrocytes displayed CDV antigen-positive, especially in their process. Some of them became apoptotic cell confirmed by TUNEL staining. Fibrous astrocytes showed more intense GFAP-positive in mild and moderate demyelinating area. Some of nervous cells located in pyramidal cell layers and nucleus nervi were in degeneration, necrosis. Satellitosis, neuronophagia and apoptotic neurons were examined by hematoxylin and eosin (HE) and TUNEL staining. The results suggested that the demyelinating changes in brain tissues infected with CDV mainly related to the metabolic disorder and apoptosis of ogliodendrocytes and astrocytes; also involved with the local disturbance of blood circulation and some neuron lost.
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