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Mapping subgenomic promoter of coat protein gene of Cucumber green mottle mosaic virus
LIU Mei, LIU Li-ming, WU Hui-jie, KANG Bao-shan, GU Qin-sheng
2020, 19 (1): 153-163.   DOI: 10.1016/S2095-3119(19)62647-X
Abstract136)      PDF in ScienceDirect      
Many plant viruses utilize subgenomic RNA as gene expression strategy, therefore mapping subgenomic promoter (SGP) is extremely important for constructing viral vectors.  Although Cucumber green mottle mosaic virus (CGMMV)-based virus vectors have been constructed, SGP of the coat protein (CP) has not yet mapped.  To this end, we firstly presumed 13 nucleotides upstream of the start codon as the transcription starting site (TSS) as previous study identified by random amplification of cDNA ends (RACE).  Secondly, the region from nucleotides –110 to +175 is the putative CP SGP, as predicted, a long stem loop structure by the secondary structure of RNA covering movement protein (MP) and CP.  To map the CGMMV CP SGP, we further constructed a series of deletion mutants according to RNA secondary structure prediction.  The deletion of TSS upstream significantly enhanced CP transcription when 105 nucleotides were retained before the CP TSS.  For the downstream of CP TSS, we analyzed the expression of enhanced green fluorescent protein (EGFP) in a series of vectors with partial deletion of the CGMMV CP and found that the nucleotides from +71 to +91 played a key role in the EGFP expression at the transcription level, while EGFP showed the highest expression level when 160 nucleotides were retained downstream of the CP TSS.  To confirm these results, we applied online software MEME to predict the motifs and cis-acting elements in the 466 nucleotides covering the sequences of deletion analysis.  Conserved motifs and relative acting elements were in regions in which transcription levels were the highest or enhanced.  To our best knowledge, this is the first mapping of CGMMV SGP.
 
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Spatio-Temporal Changes of Soil Salinity in Arid Areas of South Xinjiang Using Electromagnetic Induction
LI Xiao-ming, YANG Jing-song, LIU Mei-xian, LIU Guang-ming, YU Mei
2012, 12 (8): 1365-1376.   DOI: 10.1016/S1671-2927(00)8667
Abstract1364)      PDF in ScienceDirect      
The aim of this paper was to research the spatio-temporal changes in total soluble salt content (TS) in a typical arid region of South Xinjiang, China, where the climate is arid and soil salinization happens easily. The total soluble salt content was interpreted by measurements made in the horizontal mode with EM38 and EM31. The electromagnetic induction (EM) surveys were made three times with the apparent soil electrical conductivity (ECa) measurements taken at 3 873 locations in Nov. 2008, 4807 locations in Apr. 2009 and 6 324 locations in Nov. 2009, respectively. For interpreting the ECa measurements into total soluble salt content, calibtion sites were needed for EM survey of each time, e.g., 66 sites were selected in Nov. 2008 to measure ECa, and soils-core samples were taken by different depth layers of 0-10, 10-20 and 20-40 cm at the same time. On every time duplicate samples were taken at five sites to allevaite the local-scale variability, and soil temperatures in different layers through the profiles were also measured. Factors including TS, pH, water content, bulk density were analyzed by lab experiments. ECa calibration equations were obtained by linear regression analysis, which indicated that soil salinity was one primary concern to ECa with a determination coefficient of 0.792 in 0-10 cm layer, 0.711 in 10-20 cm layer and 0.544 in 20-40 cm layer, respectively. The maps of spatial distribution were predicted by Kriging interpolation, which showed that the high soil salinity was located near the drainage canal, which validated the trend effect caused by the irrigation canal and the drainage canal. And by comparing the soil salinity in different layers, the soluble salt accumulated to the top soil surface only in the area where the soil salinization was serious, and in the other areas, the soil salinity trended to increase from the top soil surface to 40 cm depth. Temporal changes showed that the soil salinity in November was higher than that in April, and the soil salinization trended to aggravate, especially in the top soil layer of 0- 10 cm.
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Effects of Irrigation Water Quality and Drip Tape Arrangement on Soil Salinity, Soil Moisture Distribution, and Cotton Yield (Gossypium hirsutum L.) Under Mulched Drip Irrigation in Xinjiang, China
LIU Mei-xian, LI Xiao-ming, YU Mei, WANG Jin
2012, 12 (3): 502-511.   DOI: 10.1016/S1671-2927(00)8569
Abstract1456)      PDF in ScienceDirect      
More and more attention is being focused on saline water utilization in irrigation due to the shortage of fresh water to agriculture in many regions. For purpose of reducing the risks of using of saline water for irrigation, the mechanism of soil moisture and salinity distribution and transport should be well understood for developing optimum management strategies. In this paper, field experiments were carried out at Junggar Basin, China, to study the effects of drip irrigation water quality and drip tape arrangement on distribution of soil salinity and soil moisture. Six treatments were designed, including two drip tape arrangement modes and three irrigation water concentration levels (0.24, 4.68, and 7.42 dS m-1). Results showed that, soil moisture content (SMC) directly beneath the drip tape in all treatments kept a relatively high value about 18% before boll opening stage; the SMC in the narrow strip in single tape arrangement (Ms) plot was obviously lower than that in the double tapes arrangement (Md) plot, indicating that less sufficient water was supplied under the same condition of irrigation depth, but there was no significant reduction in yield. Mulching had not significant influence on salt accumulation but the drip tape arrangement, under the same condition of irrigation water depth and quality, compared with Md, Ms reduced salt accumulation in root zone and brought about relatively high cotton yield.
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