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Heredity and gene mapping of a novel white stripe leaf mutant in wheat
LI Hui-juan, JIAO Zhi-xin, NI Yong-jing, JIANG Yu-mei, LI Jun-chang, PAN Chao, ZHANG Jing, SUN Yu-long, AN Jun-hang, LIU Hong-jie, LI Qiao-yun, NIU Ji-shan
2021, 20 (
7
): 1743-1752. DOI:
10.1016/S2095-3119(20)63345-7
Abstract
(
133
)
PDF in ScienceDirect
Spotted leaf (
spl
) mutant is a type of leaf lesion mimic mutants in plants. We obtained some lesion mimic mutants from ethyl methane sulfonate (EMS)-mutagenized wheat (
Triticum aestivum
L.) cultivar Guomai 301 (wild type, WT), and one of them was named as
white
stripe
leaf
(
wsl
) mutant because of the white stripes on its leaves. Here we report the heredity and gene mapping of this novel wheat mutant
wsl
. There are many small scattered white stripes on the leaves of
wsl
throughout its whole growth period. As the plants grew, the white stripes became more severe and the necrotic area expanded. The mutant wsl grew only weakly before the jointing stage and gradually recovered after jointing. The length and width of the flag leaf, spike number per plant and thousand-grain weight of
wsl
were significantly lower than those of the WT. Genetic analysis indicated that the trait of white stripe leaf was controlled by a recessive gene locus, named as
wsl
, which was mapped on the short arm of chromosome 6B by SSR marker assay. Four SSR markers in the F
2
population of
wsl
×CS were linked to
wsl
in the order of
Xgpw1079
–
Xwmc104
–
Xgwm508
-
wsl
–
Xgpw7651
at 7.1, 5.2, 8.7, and 4.4 cM, respectively and three SSR markers in the F
2
population of
wsl
×Jimai 22 were linked to
wsl
in the order of
Xgwm508
–
Xwmc494
–
Xgwm518
-
wsl
at 3.5, 1.6 and 8.2 cM, respectively. In comparison to the reference genome sequence of Chinese Spring (CS),
wsl
is located in a 91-Mb region from 88 Mb (
Xgwm518
) to 179 Mb (
Xgpw7651
) on chromosome 6BS. Mutant
wsl
is a novel germplasm for studying the molecular mechanism of wheat leaf development.
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Genetic mapping and expressivity of a wheat multi-pistil gene in mutant
12TP
ZHU Xin-xin, NI Yong-jing, HE Rui-shi, JIANG Yu-mei, LI Qiao-yun, NIU Ji-shan
2019, 18 (
3
): 532-538. DOI:
10.1016/S2095-3119(18)61935-5
Abstract
(
194
)
PDF
(333KB)(
202
)
We identified a wheat (Triticum aestivum L.) multi-pistil mutant from an F2 breeding population in 2012, named
12TP
(three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the multi-pistil trait. Using homozygous normal and multi-pistil lines (near-isogenic lines; NILs) derived from the original mutant
12TP
, a simple sequence repeat (SSR) marker assay located the
12TP
locus on chromosome arm 2DL. Four SSR markers were linked to
12TP
and their order was
Xcfd233→Xcfd62-12TP→Xwmc41→Xcfd168
at 15.85, 10.47, 2.89, and 10.37 cM, respectively. The average genetic expressivity of the trait ‘three pistils in one floret’ was more than 98% in seven homozygous
12TP
lines; however, the average genetic expressivity in heterozygous F1 plants was about 49%. Thus, the
12TP
is a semi-dominant gene locus, which differ from all previously reported multi-pistil mutants. Mutant
12TP
is a new useful germplasm for study of wheat floral development and for breeding of high yield wheat.
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Determination of Optimum Growing Degree-Days (GDD) Range Before Winter for Wheat Cultivars with Different Growth Characteristics in North China Plain
LI Qiao-yun, LIU Wan-dai, LI Lei, NIU Hong-bin, MA Ying
2012, 12 (
3
): 405-415. DOI:
10.1016/S1671-2927(00)8558
Abstract
(
1888
)
PDF in ScienceDirect
To provide base for adjusting the sowing date, achieving the yield potential of wheat cultivars with different growth characteristics, and improving the utilization rate of natural resource in the North China Plain (NCP), a 4-yr field experiment of growing degree-days (GDD) before winter (realized through different sowing dates) with three wheat (Triticum aestivum L.) cultivars of each type of semi-winterness and weak springness was carried out at 20 test experimental sites (32°4´N- 36°1´N) of Henan Province in the NCP. The results showed that: (i) yield of semi-winterness wheat was significantly higher than weak springness wheat (**P<0.01); (ii) there was a quadratic regression between the yield and GDD before winter. According to the regression equation, the optimum GDD range with high yield of semi-winterness and weak springness wheats was 750-770 and 570-590°C d, respectively; (iii) under the optimum GDD condition, the foliar age on the main stem of semi-winterness and weak springness wheats was 7.67-7.91 and 6.36-6.86 leaves, respectively, calculated by the linear regression equation between foliar age and GDD before winter; (iv) both semi-winterness and weak springness wheats were in the double ridge stage of spike differentiation under the condition of the optimum GDD range, and at this time, the foliar age on the main stem of semi-winterness and weak springness wheats was about 7.80 and 6.07 leaves, respectively, which was consistent with the results calculated by the liner regression equation. Therefore, we could consider that the sowing date is appropriate if the foliar age is about 7.8 and 6.3 leaves for semi-winterness and weak springness wheats, respectively. According to the results of this study, choosing semi-winterness wheat and planting 7- 10 d earlier would improve yield and natural resource utilization in NCP.
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