The P-type plasma membrane (PM) H⁺-ATPases (HAs) are crucial for plant development, growth, and defense.  The HAs have been thoroughly characterized in many different plants.  However, despite their importance, the functions of HAs in germination and seed dormancy (SD) have not been validated in wheat.  Here, we identified 28 TaHA genes (TaHA1-28) in common wheat, which were divided into five subfamilies.  An examination of gene expression in strong- and weak-SD wheat varieties led to the discovery of six candidate genes (TaHA7/-12/-14/-16/-18/-20).  Based on a single nucleotide polymorphism (SNP) mutation (C/T) in the TaHA7 coding region, a CAPS marker (HA7) was developed and validated in 168 wheat varieties and 171 Chinese mini-core collections that exhibit diverse germination and SD phenotypes.  We further verified the roles of the two allelic variations of TaHA7 in germination and SD using wheat mutants mutagenized with ethyl methane sulphonate (EMS) in ‘Jimai 22’ and ‘Jing 411’ backgrounds, and in transgenic Arabidopsis lines.  TaHA7 appears to regulate germination and SD by mediating gibberellic acid (GA) and abscisic acid (ABA) signaling, metabolism, and biosynthesis.  The results presented here will enable future research regarding the TaHAs in wheat.