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A novel short transcript isoform of chicken IRF7 negatively regulates interferon-β production
MA Yu-chen, CHEN Hua-yuan, GAO Shen-yan, ZHANG Xiao-zhan, LI Yong-tao, YANG Xia, ZHAO Jun, WANG Zeng
2023, 22 (7): 2213-2220.   DOI: 10.1016/j.jia.2022.12.015
Abstract136)      PDF in ScienceDirect      
Type I interferon (IFN-I) provides an important first line to protect avian species against pathogens invasion. IFN regulatory factor 7 (IRF7) has been identified as the most important regulator for both DNA and RNA virus-induced IFN-I production in chickens. Although four splicing variants of IRF7 have been identified in mammals, it is still unclear whether alternative splicing patterns and the function of IRF7 isoform(s) exist in chickens. In this study, we reported a novel short transcript isoform of chicken IRF7 (chIRF7), termed chIRF7-iso, which contained an intact N-terminal DNAbinding domain (DBD) and 14 amino acids different from chIRF7 in the C-terminal. Overexpression of chIRF7 in chicken leghorn male hepatocellular (LMH) cells activated the IFN-β promoter and significantly inhibited Newcastle disease virus (NDV) and fowl adenovirus serotype 4 (FAdV-4) replication. Conversely, overexpression of chIRF7-iso blocked the IFN-β promoter activity and was favorable for NDV and FAdV-4 replication in vitro. Collectively, our results confirm that a novel chIRF7 isoform-mediated negative regulates IFN-β production, which will contribute to understanding the role of chIRF7 in innate antiviral response in chicken.
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Effects of different types of slow- and controlled-release fertilizers on rice yield
WU Qiong, WANG Yu-hui, DING Yan-feng, TAO Wei-ke, GAO Shen, LI Quan-xin, LI Wei-wei, LIU Zheng-hui, LI Gang-hua
2021, 20 (6): 1503-1514.   DOI: 10.1016/S2095-3119(20)63406-2
Abstract148)      PDF in ScienceDirect      
This experiment explored the effects of single application of seven types of slow- and controlled-release fertilizers on rice yield and various population characteristics.  Based on a study of the nitrogen (N) release characteristics of these fertilizers, pot experiments were conducted in 2018 and 2019 with split fertilization (CK, urea applied split equally at basal and panicle initiation stages, respectively) as control, which assessed the effects on SPAD value, yield and yield components, dynamic changes of rice tillers and dry matter accumulation.  The results showed that the N release characteristics of different types of slow- and controlled-release fertilizers were significantly different.  Polymer-coated urea (PCU) showed a controlled-release mode and provided sustained release throughout the whole growth stages.  Sulfur-coated urea (SCU) exhibited a slow-release mode, providing insufficient release at the middle and late stages.  Urease inhibitor urea (AHA) and urea-formaldehyde (UF) yielded a rapid-release mode, with an explosive N release at the early stage and no release at the middle and late stages.  These results showed that PCU delayed the peak seedling stage.  Compared with CK, dry matter accumulation and SPAD showed no significant differences, and due to the continuous release of N throughout the growth stages, rice yield, spikelets per panicle, seed setting rate, and 1 000-grain weight were all increased.  Owing to the lack of N supply at the late stage and the low number of spikelets, SCU led to a reduction of rice yield, which is nevertheless not statistically significant.  AHA and UF were susceptible to environmental factors and had varying effects on rice yield.  The results of this experiment indicated that given a fixed amount of N applied in a pot, the stronger the N supply capacity and the longer the effective duration time of the fertilizer, the higher the dry matter accumulation at the late growth stage, and the higher the rice yield.
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