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Fine mapping and characterization of a major QTL for grain length, QGl.cau-2D.1, that has pleiotropic effects in synthetic allohexaploid wheat
Mingming Wang, Jia Geng, Zhe Zhang, Zihan Zhang, Lingfeng Miao, Tian Ma, Jiewen Xing, Baoyun Li, Qixin Sun, Yufeng Zhang, Zhongfu Ni
2024, 23 (9):
2911-2922.
DOI: 10.1016/j.jia.2023.09.009
Grain size is one of the determinants of grain yield, and identifying the genetic loci that control grain size will be helpful for increasing grain yield. In our previous study, a quantitative trait locus (QTL) for grain length (GL), QGl.cau-2D.1, was identified from an F2 population developed from the cross between the natural (TAA10) and synthetic (XX329) allohexaploid wheat. In the present study, we mainly fine mapped and validated its genetic effects. To this end, multiple near-isogenic lines (NILs) were obtained through marker-assisted selection with TAA10 as the recurrent parent. The secondary populations derived from 25 heterozygous recombinants were used for fine mapping of QGl.cau-2D.1, and the allele from XX329 significantly increased GL, thousand-grain weight (TGW), total spikelet number per spike (TSN) and spike compactness (SC). Using NILs for XX329 (2D+) and TAA10 (2D−), we determined the genetic and pleiotropic effects of QGl.cau-2D.1. The target sequences were aligned with the wheat reference genome RefSeq v2.1 and spanned an ~0.9 Mb genomic region. TraesCS2D03G0114900 (ortholog of Os03g0594700) was predicted as the candidate gene based on whole-genome re-sequencing and expression analyses. In summary, the map-based cloning of QGl.cau-2D.1 will be useful for improving grain weight with enhanced GL and TSN.
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