The study explores the antifungal properties of functionalized multi-walled carbon nanotubes (f-MWCNTs) against Fusarium oxysporum f. sp. tuberosi, revealing a concentration-dependent impact, with the lowest concentration suppressing mycelial development.  The peaks at 2θ°=7.92° and 25.85° reveal the presence of MWCNTs.  Furthermore, the bonding extremes at 3,194 and 2,441 cm^–1 and the peak at 3,573 cm^–1 are hydrogen-bonded.  The peak at 3,756 cm^–1 demonstrates the vibration of OH stretching to confirm the functionalization of MWCNTs.  MWCNTs at 308 nm show a peak with much higher UV energy.  This is because of the different plasmonic vibrations that the free electrons of multi-wall carbon nanotubes exhibit at about 308 nm.  Scanning electron microscope (SEM) analysis revealed mycelial structure distortions, revealing inhibitory mechanisms of f-MWCNTs and their interaction with F. oxysporum f. sp. tuberosi, providing insights into their complex behavior.  Multi-walled carbon nanotubes (MWCNTs) showed anti-oxidative properties, indicating potential multifaceted modes of action, as evidenced by 2´,7´-dichlorofluorescein diacetate dye testing.  The current study analyzed bioactive molecules in F. oxysporum f. sp. tuberosi extracts by gas-chromatography-mass spectrometry (GC-MS) analysis, showing six metabolites having antimicrobial, cytotoxic, and antioxidant properties.  However, exposure to f-MWCNTs reduced these potent molecule concentrations, highlighting the significant impact of f-MWCNTs on F. oxysporum f. sp. tuberosi biochemical arsenal.  This is the first report that checked the antifungal, and antioxidant activity and of a lesser concentration of metabolites produced after the action of f-MWCNTs in F. oxysporum f. sp. tuberosi.  This research highlights the potential of f-MWCNTs as antifungal agents, paving the way for innovative strategies in combating fungal pathogens and developing effective treatments.