蔗糖非发酵相关蛋白激酶2（SnRK2）是植物特有的一类丝氨酸/苏氨酸蛋白激酶，其能够应对大量不利的环境刺激。之前研究报道了小麦TaSnRK2.4响应非生物逆境胁迫，提高了转基因拟南芥的多重抗性。本研究将揭示TaSnRK2.4的抗逆机理并发掘新的功能。TaSnRK2.4s分别被定位于3A、3B和3D染色体，这3种基因组序列均被克隆。多态性检测结果表明，TaSnRK2.4-3A和TaSnRK2.4-3B分别有1处和13处变异位点，TaSnRK2.4-3D未发现变异位点。基于其中3处变异位点，开发了标记2.4AM1、2.4BM1和2.4BM2。关联分析结果表明，TaSnRK2.4-3A和TaSnRK2.4-3B均与千粒重显著关联，其中SNP3A-T和SNP3B-C是高千粒重的优异等位变异。酵母双杂交和荧光素酶互补成像试验表明，TaSnRK2.4和逆境响应蛋白TaLTP3互作，进而得出TaSnRK2.4通过激活TaLTP3参与抗逆。我们的研究显示了TaSnRK2.4在增产抗逆方面具有巨大潜力。

The stress-associated protein (SAP) multigene family is conserved in both animals and plants.  Its function in some animals and plants are known, but it is yet to be deciphered in wheat (Triticum aestivum L.).  We identified the wheat gene TaSAP17-D, a member of the SAP gene family with an AN1/AN1 conserved domain.  Subcellular localization indicated that TaSAP17-D localized to the nucleus, cytoplasm, and cell membrane.  Expression pattern analyses revealed that TaSAP17-D was highly expressed in seedlings and was involved in NaCl response, polyethylene glycol (PEG), cold, and exogenous abscisic acid (ABA).  Constitutive expression of TaSAP17-D in transgenic Arabidopsis resulted in enhanced tolerance to salt stress, confirmed by improved multiple physiological indices and significantly upregulated marker genes related to salt stress response.  Our results suggest that TaSAP17-D is a candidate gene that can be used to protect crop plants from salt stress.  

   Lipid transfer protein (LTP) is a kind of small molecular protein, which is named for its ability to transfer lipid between cell membranes. It has been proved that the protein is involved in the responding to abiotic stresses. In this study, TaLTP-s, a genomic sequence of TaLTP was isolated from A genome of wheat (Triticum aestivum L). Sequencing analysis exhibited that there was no diversity in the coding region of TaLTP-s, but seven single nucleotide polymorphisms (SNPs) and 1 bp insertion/deletion (InDel) were detected in the promoter regions of different wheat accessions. Nucleotide diversity (π) in the region was 0.00033, and linkage disequilibrium (LD) extended over almost the entire TaLTP-s region in wheat. The dCAPS markers based on sequence variations in the promoter regions (SNP-207 and SNP-1696) were developed, and three haplotypes were identified based on those markers. Association analysis between the haplotypes and agronomic traits of natural population consisted of 262 accessions showed that three haplotypes of TaLTP-s were significantly associated with plant height (PH). Among the three haplotypes, HapIII is considered as the superior haplotype for increasing plant height in the drought stress environments. The G variance at the position of 207 bp could be a superior allele that significantly increased number of spikes per plant (NSP). The functional marker of TaLTP-s provide a tool for marker-assisted selection regarding to plant height and number of spikelet per plant in wheat.