TaSnRK2.4 is a vital regulator in control of thousand-kernel weight and response to abiotic stress in wheat

doi:10.1016/S2095-3119(19)62830-3

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (1): 46-54.DOI: 10.1016/S2095-3119(19)62830-3

所属专题：麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources

收稿日期:2019-08-21 出版日期:2021-01-01 发布日期:2020-12-13

TaSnRK2.4 is a vital regulator in control of thousand-kernel weight and response to abiotic stress in wheat

MIAO Li-li^{1, 2}, LI Yu-ying³, ZHANG Hong-juan³, ZHANG Hong-ji², LIU Xiu-lin⁴, WANG Jing-yi³, CHANG Xiao-ping³, MAO Xin-guo³, JING Rui-lian³

¹Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin 150086, P.R.China
² Institute of Crop Resources, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.China
³ Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
⁴ Institute of Soybean, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.China

Received:2019-08-21 Online:2021-01-01 Published:2020-12-13
Contact: Correspondence MAO Xin-guo, Tel: +86-10-82105829, E-mail: maoxinguo@caas.cn; JING Rui-lian, Tel: +86-10-82105829, E-mail: jingruilian@caas.cn
Supported by:
This work was supported by the National Key Research and Development Program of China (2017YFD0300202), the National Natural Science Foundation of China (31571660), the Heilongjiang Postdoctoral Financial Assistance, China (LBH-Z17200), and the Research Project of Heilongjiang Academy of Agricultural Sciences, China (2018YYYF018).

摘要/Abstract

摘要：

蔗糖非发酵相关蛋白激酶2（SnRK2）是植物特有的一类丝氨酸/苏氨酸蛋白激酶，其能够应对大量不利的环境刺激。之前研究报道了小麦TaSnRK2.4响应非生物逆境胁迫，提高了转基因拟南芥的多重抗性。本研究将揭示TaSnRK2.4的抗逆机理并发掘新的功能。TaSnRK2.4s分别被定位于3A、3B和3D染色体，这3种基因组序列均被克隆。多态性检测结果表明，TaSnRK2.4-3A和TaSnRK2.4-3B分别有1处和13处变异位点，TaSnRK2.4-3D未发现变异位点。基于其中3处变异位点，开发了标记2.4AM1、2.4BM1和2.4BM2。关联分析结果表明，TaSnRK2.4-3A和TaSnRK2.4-3B均与千粒重显著关联，其中SNP3A-T和SNP3B-C是高千粒重的优异等位变异。酵母双杂交和荧光素酶互补成像试验表明，TaSnRK2.4和逆境响应蛋白TaLTP3互作，进而得出TaSnRK2.4通过激活TaLTP3参与抗逆。我们的研究显示了TaSnRK2.4在增产抗逆方面具有巨大潜力。

Abstract:

Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) is a plant-specific serine/threonine kinase involved in response to adverse environmental stimuli. Previous studies showed that TaSnRK2.4 was involved in response to abiotic stresses and conferred enhanced tolerance to multiple stresses in Arabidopsis. Further experiments were performed to decipher the underlying mechanisms and discover new functions. The genomic sequences of TaSnRK2.4s locating on chromosome 3A, 3B and 3D were obtained. Sequencing identified one and 13 variations of TaSnRK2.4-3A and TaSnRK2.4-3B, respectively, but no variation was detected in TaSnRK2.4-3D. The markers 2.4AM1, 2.4BM1 and 2.4BM2 were developed based on three variations. Association analysis showed that both TaSnRK2.4-3A and TaSnRK2.4-3B were significantly associated with thousand-kernel weight (TKW), and that SNP3A-T and SNP3B-C were favorable alleles for higher TKW. Yeast two-hybrid and split luciferase assays showed that TaSnRK2.4 physically interacted with abiotic stress responsive protein TaLTP3, suggesting that TaSnRK2.4 enhanced abiotic stress tolerance by activating TaLTP3. Our studies suggested that TaSnRK2.4 have potential in improving TKW and response to abiotic stress.

Key words: TaSnRK2.4 , association analysis , TKW , abiotic stress

. [J]. Journal of Integrative Agriculture, 2021, 20(1): 46-54.

MIAO Li-li, LI Yu-ying, ZHANG Hong-juan, ZHANG Hong-ji, LIU Xiu-lin, WANG Jing-yi, CHANG Xiao-ping, MAO Xin-guo, JING Rui-lian. TaSnRK2.4 is a vital regulator in control of thousand-kernel weight and response to abiotic stress in wheat[J]. Journal of Integrative Agriculture, 2021, 20(1): 46-54.

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TaSnRK2.4 is a vital regulator in control of thousand-kernel weight and response to abiotic stress in wheat

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