A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis

doi:10.1016/S2095-3119(17)61681-2

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (03): 507-516.DOI: 10.1016/S2095-3119(17)61681-2

XU Qiao-fang, MAO Xin-guo, WANG Yi-xue, WANG Jing-yi, XI Ya-jun, JING Rui-lian

收稿日期:2017-02-24 出版日期:2018-03-20 发布日期:2018-03-01

A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis

XU Qiao-fang^{1, 2}, MAO Xin-guo², WANG Yi-xue², WANG Jing-yi², XI Ya-jun¹, JING Rui-lian²

¹ College of Agronomy, Northwest A&F University, Yangling 712100, P.R.China
² National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2017-02-24 Online:2018-03-20 Published:2018-03-01
Contact: Correspondence XI Ya-jun, Tel: +86-29-87082845, E-mail: xiyajun11@126.com; JING Rui-lian, Tel: +86-10-82105829, E-mail: jingruilian@caas.cn
About author:XU Qiao-fang, Mobile: +86-13011216511, E-mail: xqfang2014@163.com
Supported by:
This work was supported by the National Key Research and Development Program of China (2016YFD0100605), and the Agricultural Science and Technology Innovation Program, China (ASTIP).

摘要/Abstract

Abstract: The stress-associated protein (SAP) multigene family is conserved in both animals and plants. Its function in some animals and plants are known, but it is yet to be deciphered in wheat (Triticum aestivum L.). We identified the wheat gene TaSAP17-D, a member of the SAP gene family with an AN1/AN1 conserved domain. Subcellular localization indicated that TaSAP17-D localized to the nucleus, cytoplasm, and cell membrane. Expression pattern analyses revealed that TaSAP17-D was highly expressed in seedlings and was involved in NaCl response, polyethylene glycol (PEG), cold, and exogenous abscisic acid (ABA). Constitutive expression of TaSAP17-D in transgenic Arabidopsis resulted in enhanced tolerance to salt stress, confirmed by improved multiple physiological indices and significantly upregulated marker genes related to salt stress response. Our results suggest that TaSAP17-D is a candidate gene that can be used to protect crop plants from salt stress.

Key words: SAP , multigene family , TaSAP17-D , salt tolerance , wheat

. [J]. Journal of Integrative Agriculture, 2018, 17(03): 507-516.

XU Qiao-fang, MAO Xin-guo, WANG Yi-xue, WANG Jing-yi, XI Ya-jun, JING Rui-lian. A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis[J]. Journal of Integrative Agriculture, 2018, 17(03): 507-516.

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A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis

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