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1.
Impacts of the COVID-19 pandemic on rural poverty and policy responses in China
LUO Ren-fu, LIU Cheng-fang, GAO Jing-jing, WANG Tian-yi, ZHI Hua-yong, SHI Peng-fei, HUANG Ji-kun
Journal of Integrative Agriculture 2020, 19 (
12
): 2946-2964. DOI:
10.1016/S2095-3119(20)63426-8
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Given the sudden outbreak of the COVID-19 pandemic, a timely study on the impacts of and policy response to the pandemic on rural poverty in China is critically important because China has aimed to completely eradicate extreme poverty by the end of 2020. This paper uses data from the latest round of a nationally representative household panel survey to examine the impacts of the pandemic on rural poverty in China. Our data show that 11.9% of sample households were ever officially registered as poor households between 2013 and 2019, and this poverty incidence fell to 2.7% by the end of 2019. In the middle February of 2020, 23% of the households who have graduated from poverty since 2013 perceived that they would fall back into poverty due to the COVID-19. Among those never poor households, 7.1% perceived that they would possibly fall into poverty due to the pandemic. Results from both descriptive and multivariate analyses consistently show the interruptions that the pandemic caused in off-farm employment is an important channel that led households to perceive of falling back into or falling into poverty. We also find households in the bottom four quintiles when ranked in terms of household income per capita are much more likely to perceive themselves of falling back into or falling into poverty during this pandemic than those in the richest quintile. Meanwhile, our results show that the education and age of household heads, as well as being from Hubei Province matter in explaining household perception about falling back into or falling into poverty in some cases but not all. The paper concludes with a set of policy responses that China has taken to mitigate the impact of COVID-19 pandemic on poverty alleviation.
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2.
Transcriptome profiling using RNA-seq to provide insights into foxtail millet seedling tolerance to short-term water deficit stress induced by PEG-6000
XU Bing-qin, GAO Xiao-li, GAO Jin-feng, LI Jing, YANG Pu, FENG Bai-li
Journal of Integrative Agriculture 2019, 18 (
11
): 2457-2471. DOI:
10.1016/S2095-3119(19)62576-1
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144
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3.
Systemic acquired resistance,
NPR1
, and pathogenesis-related genes in wheat and barley
WANG Xiao-dong, BI Wei-shuai, GAO Jing, YU Xiu-mei, WANG Hai-yan, LIU Da-qun
Journal of Integrative Agriculture 2018, 17 (
11
): 2468-2476. DOI:
10.1016/S2095-3119(17)61852-5
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384
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In
Arabidopsis
, systemic acquired resistance (SAR) is established beyond the initial infection by a pathogen or is directly induced by treatment with salicylic acid (SA) or its functional analogs, 2,6-dichloroisonicotinic acid (INA) and benzothiadiazole (BTH). NPR1 protein is considered the master regulator of SAR in both SA signal sensing and transduction. In wheat (
Triticum aestivum
) and barley (Hordeum vulgare), both pathogen infection and BTH treatment can induce broad-spectrum resistance to various diseases, including powdery mildew, leaf rust,
Fusarium
head blight, etc. However, three different types of SAR-like responses including acquired resistance (AR), systemic immunity (SI), and BTH-induced resistance (BIR) seem to be achieved by activating different gene pathways. Recent research on wheat and barley
NPR1
homologs in AR and SI has provided the initial clue for understanding the mechanism of SAR in these two plant species. In this review, the specific features of AR, SI, and BIR in wheat and barley were summarized and compared with that of SAR in model plants of
Arabidopsis
and rice. Research updates on downstream genes of SAR, including pathogenesis-related (PR) and BTH-induced genes, were highlighted.
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4.
Reducing nitrogen fertilization of intensive kiwifruit orchards decreases nitrate accumulation in soil without compromising crop production
LU Yong-li, KANG Ting-ting, GAO Jing-bo, CHEN Zhu-jun, ZHOU Jian-bin
Journal of Integrative Agriculture 2018, 17 (
06
): 1421-1431. DOI:
10.1016/S2095-3119(17)61899-9
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460
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Excessive nitrogen (N) fertilization of high value horticultural crops is a common problem that not only increases the cost to farmers, but also negatively affects crop growth and the environment. A three-year field experiment was conducted in an intensive kiwifruit orchard in Shaanxi Province, China to compare the effects of reduced N fertilization applied as urea (U), and controlled release urea (CRU) on the N nutrition of kiwi vines, fruit yield and quality, and nitrate-N accumulation in the soil profile. The three treatments included a conventional N application rate (CF-U, 900 kg N ha
–1
yr
–1
as urea), two reduced N fertilization treatments where the amount of N fertilizer applied as U and CRU was reduced by 25% in 2013 and 2014, and by 45% in 2015. The 25 and 45% reduced N treatments had no adverse effects on the N concentrations in leaves and pruning branches and the fruit yield and quality of kiwi vines. However, they significantly enhanced the partial factor productivity of applied N (PFP
N
) and the economic benefits, and reduced nitrate accumulation in the 0–200 cm soil profile. The same benefits of reduced N fertilization were observed for both the U and CRU treatments, but the CRU treatment had the added benefit of decreasing the loss of nitrate through leaching. We concluded that the current level of N fertilization in kiwi orchards is very excessive, and reducing the N fertilizer rate by 25–45% could not only guarantee fruit yield, but also reduce N accumulation and loss.
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5.
MAPKs and acetyl-CoA are associated with
Curvularia lunata
pathogenicity and toxin production in maize
NI Xuan, GAO Jin-xin, YU Chuan-jin, WANG Meng, Sun Jia-nan, LI Ya-qian, CHEN Jie
Journal of Integrative Agriculture 2018, 17 (
01
): 139-148. DOI:
10.1016/S2095-3119(17)61697-6
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633
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Mitogen-activated protein kinase (MAPK) cascades play an important role in extracellular signal transduction and are involved in the pathogenicity of fungal pathogens to host plants. In
Curvularia lunata
, the roles of two MAPK genes,
Clk1
and
Clm1
, have already been studied.
Clk1
is involved in conidia formation and pathogenicity, and
Clm1
is closely related to pathogen cell wall formation and pathogenicity to maize leaves. In this study, a third
C. lunata
MAPK gene,
Clh1
, which is homologous to
hog1
, was successfully cloned. We found that a
Clh1
deletion mutant had lower intracellular glycerol accumulation than the wild-type stain and was unable to grow normally under osmotic stress conditions. Furthermore, the deletion mutants of three
C. lunata
MAPK genes (
Clk1
,
Clm1
and
Clh1
) had lower levels of acetyl-CoA, which is an important intermediate product in the synthesis of melanin and furan toxin, and down-regulated expression of pathogenicity-associated genes. Furthermore, pathogenicity and the ability to produce toxin were restored after adding acetyl-CoA to the culture medium, suggesting that acetyl-CoA is closely involved in the pathogen MAPK signaling pathway.
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6.
Sod
gene of
Curvularia lunata
is associated with the virulence in maize leaf
GAO Shi-gang, NI Xuan, LI Ying-ying, FU Ke-he, YU Chuan-jin, GAO Jin-xin, WANG Meng, LI Ya-qian, CHEN Jie
Journal of Integrative Agriculture 2017, 16 (
04
): 874-883. DOI:
10.1016/S2095-3119(16)61513-7
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976
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Curvularia
leaf spot, caused mainly by
Curvularia lunata
, is a widespread plant disease in China. In the recent years, directional host selection by the pathogen, which likely results in the virulence differentiation in pathogens, is widely reported. Among the hallmarks potentially associated to pathogen variation in virulence, superoxide dismutase gene
Sod
has been found to be closely related to the enhancement of virulence. In the present study, the full-length of
Sod
was obtained
via
Blastn alignment against GenBank and the whole genome of
C
.
lunata
. In order to understand the role of
Sod
in the virulence variation in
C
.
lunata
, targeted gene disruption was performed to construct
Sod
mutants. The cell wall degrading enzyme (CWDE) activities and toxin production of
ΔSod
were not distinctly different from wild-type strain CX-3 and its complon. However, at an early stage of infection,
ΔSod
virulence appeared to be lower than CX-3 and the complon, while at a later stage, its virulence gradually returned to the level of CX-3 and the complon. Furthermore, the melanin production of
ΔSod
was significantly reduced compared to CX-3 and the complon, suggesting that
Sod
gene influences the virulence by regulating melanin production at an early stage of infection but is not essential for pathogenicity. However, the disruption of
Sod
did not significantly affect the transcriptional expression of the melanin biosynthesis-associated genes,
brn1
and
scd
. Therefore, we infer that
Sod
in
C
.
lunata
are involved, to some extent, with the virulence in maize leaf, but still needs further studies to have a clear understanding of its mechanism.
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7.
Effect of Fuzheng Jiedu granule on immunological function and level of immune-related cytokines in immune-suppressed mice
YU Zhan-mei, HUANG Xian-hui, YAN Chao-qun, GAO Jin, LIANG Zi-sen
Journal of Integrative Agriculture 2016, 15 (
3
): 650-657. DOI:
10.1016/S2095-3119(14)60971-0
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1652
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Fuzheng Jiedu granule exhibits a number of health benefits and it is thought that the mechanisms involved in these effects are due to the modulation of immunity. In this article, we studied the effect of Fuzheng Jiedu granule on immunological function and the expression of immune-related cytokines in immune-suppressed mice. 72 mice were randomly divided into six groups, with 12 in each group. The control groups included an untreated group, a negative control group (Cyclophosphamide) and a positive control group (Astragalus polysaccharide). There were three treated groups, which were given different doses of Fuzheng Jiedu granule: a low dose (100 mg kg–1), a medium dose (400 mg kg–1) and a high dose (600 mg kg–1). With the exception of the untreated control animals, each group received an intraperitoneal injection of Cyclophosphamide (100 mg kg–1) for 3 days to establish the immune-suppressed model. Mice were then treated for 19 consecutive days and, 24 h after the last treatment, blood was taken for the eyeballs and serum separation was performed. Analysis was made of the levels of related cytokines (IgA, IgG, IgM, IL-6, IFN-γ, C3, C4 and TNF-α), the transformation of lymphocytes and the immune organ indexes. The results showed that Fuzheng Jiedu granule can improve the levels of cytokines, the rate of proliferation of lymphocytes and the immune organ indexes of immune-suppressed mice.
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8.
Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean
CHENG Wen, GAO Jin-shan, FENG Xing-xing, SHAO Qun, YANG Su-xin, FENG Xian-zhong
Journal of Integrative Agriculture 2016, 15 (
10
): 2228-2236. DOI:
10.1016/S2095-3119(15)61312-0
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1775
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Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M2 population. Two of these mutants,
Gmdwf1
(
Glycine max dwarf 1
) and
Gmdwf2
(
Glycine max dwarf 2
), were investigated in this study. Genetic analysis showed that both mutants were inherited in a recessive manner and their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping. Further fine mapping study proved that the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously. The dwarfism of Gmdwf1 could not be rescued by gibberellin (GA) and brassinolide (BR) treatments, which indicated that the biosynthesis of these hormones was not deficient in
Gmdwf1
.
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9.
Relations Between Photosynthetic Parameters and Seed Yields of Adzuki Bean Cultivars (Vigna angularis)
SONG Hui, GAO Jin-feng, GAO Xiao-li, DAI Hui-ping, ZHANG Pan-an, FENG Bai-li, WANG Peng-ke
Journal of Integrative Agriculture 2012, 12 (
9
): 1453-1461. DOI:
10.1016/S1671-2927(00)8677
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1472
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The study comparatively examined the leaf photosynthetic capacities of different adzuki bean cultivars, high-yield 2000-75 and Jihong 9218, and low-yield Hongbao 1 and Wanxuan 1 from flowering to ripening. It showed that after flowering, the leaves of the cultivars gradually aged, the leaf chlorophyll (Chl.), soluble protein (SP) contents, net photosynthetic rates (Pn), transpiration rates (Tr) and stomatal conductance (Gs) of the cultivars tended to decline, but the leaf intercellular CO2 concentration (Ci) of the cultivars tended to rise. The leaf photosynthetic capacities of the cultivars decreased gradually from the lower to the upper nodes. The dry seed yields of the cultivars were positively correlated with their leaf Chl., SP, Pn, and Tr and Gs, and negatively associated with their leaf Ci. At the late growth stages, the high-yield cultivars maintained higher leaf Chl. contents, SP contents, Pn, Tr, and Gs than the low-yield cultivars, indicating that leaf photosynthetic capacity was one of important yield-affecting factors of adzuki bean. Therefore, it was important for a crop at the crucial stage of yield formation to maintain a high leaf chlorophyll content and a high leaf photosynthetic capacity and delay leaf aging.
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